Investigation of
            <i>Copaifera</i>
            Genus as a New Source of Antimycobaterial Agents

Alves, J.A.; Abrão, Fariza; Moraes, Thaís da Silva; Damasceno, Jaqueline Lopes; Moraes, Marcos F.; Veneziani, Rodrigo Cássio Sola; Ambrósio, Sérgio Ricardo; Bastos, Jairo Kenupp; Miranda, Mayker Lázaro Dantas; Martins, Carlos Henrique Gomes

doi:10.2144/fsoa-2020-0018

Cited by 10 publications

(7 citation statements)

References 48 publications

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“…MIC values of IBC against mycobacteria species were determined according to Palomino and collaborators protocol, with slight modifications [ 19 , 20 ]. A stock solution of IBC was prepared in DMSO and diluted in Middlebrook 7H9 broth to achieve final concentrations ranging from 7.8 to 1000 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption

et al. 2022

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Isobavachalcone (IBC) is a natural prenylated chalcone with a broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity of IBC against Gram-positive, Gram-negative and mycobacterial species. IBC was active against Gram-positive bacteria, mainly against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA), with minimum inhibitory concentration (MIC) values of 1.56 and 3.12 µg/mL, respectively. On the other hand, IBC was not able to act against Gram-negative species (MIC > 400 µg/mL). IBC displayed activity against mycobacterial species (MIC = 64 µg/mL), including Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii. IBC was able to inhibit more than 50% of MSSA and MRSA biofilm formation at 0.78 µg/mL. Its antibiofilm activity was similar to vancomycin, which was active at 0.74 µg/mL. In order to study the mechanism of the action by fluorescence microscopy, the propidium iodide (PI) and SYTO9 fluorophores indicated that IBC disrupted the membrane of Bacillus subtilis. Toxicity assays using human keratinocytes (HaCaT cell line) showed that IBC did not have the capacity to reduce the cell viability. These results suggested that IBC is a promising antibacterial agent with an elucidated mode of action and potential applications as an antibacterial drug and a medical device coating.

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Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption

et al. 2022

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“…The oleoresin of Copaifera reticulata has several compounds that guarantee its various biological activities, and these are mainly attributed to its main compounds [ 2 , 3 , 5 , 12 , 14 , 15 , 37 ]. In this study, when Copaifera reticulata Ducke was characterized by gas chromatography mass spectrometry (GC-MS) [ 10 ], it presented β-caryophyllene as a major component (37.3%), which is a ligand of the cannabinoid receptor 2 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Copaiba oleoresin exhibits a variety of biological actions, including anti-inflammatory [ 2 , 12 , 14 ], antibacterial [ 15 ], antifungal [ 16 ], and larvicidal [ 17 ] activities. Copaiba application in dentistry is a relatively new area of study that is constantly expanding, and most studies have investigated the effects of copaiba on oral pathogens in vitro [ 11 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Copaiba in Damage to the Alveolar Bone in a Model of Periodontitis Induced in Rats

et al. 2022

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Several studies have investigated the effects of natural products in the treatment of diseases. Traditional Amazonian populations commonly use copaiba due to its well-known anti-inflammatory, antibacterial, and healing properties. In this study, we aimed to investigate the effects of systemic administration of copaiba oleoresin (Copaifera reticulata Ducke) on ligature-induced periodontitis in rats. To do so, 21 adult rats were divided into three groups (n = 7 each): a control group, ligature-induced periodontitis group, and ligature-induced periodontitis group treated with copaiba oleoresin (200 mg/kg/day). The ligature remained from day 0 to 14, and the copaiba oleoresin was administered via oral gavage during the last seven days. On day 14, the animals were euthanized, and mandibles were collected for histopathological evaluation and microcomputed tomography analysis. Our data showed that the administration of copaiba considerably reduced the inflammatory profile. Moreover, copaiba oleoresin limited alveolar bone loss, increased trabecular thickness and bone-to-tissue volume ratio, and decreased the number of trabeculae compared with those of the untreated experimental periodontitis group. Our findings provide pioneering evidence that supports the potential of copaiba oleoresin in reducing periodontitis-induced alveolar bone damage in rats.

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“…The MIC values of LCC against mycobacteria species were determined according to the protocol of Palomino and collaborators, with slight modifications [52,53]. A stock solution of LCC was prepared in DMSO and diluted in Middlebrook 7H9 broth to achieve the final concentrations ranging from 7.8 µg/mL to 1000 µg/mL.…”

Section: Antibacterial Assaysmentioning

confidence: 99%

Synthesis, Antibacterial Effects, and Toxicity of Licochalcone C

Ozanique,

Helena,

Menezes

et al. 2024

Pharmaceuticals

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Drug-resistant bacteria constitute a big barrier against current pharmacotherapy. Efforts are urgent to discover antibacterial drugs with novel chemical and biological features. Our work aimed at the synthesis, evaluation of antibacterial effects, and toxicity of licochalcone C (LCC), a naturally occurring chalcone. The synthetic route included six steps, affording a 10% overall yield. LCC showed effects against Gram-positive bacteria (MIC = 6.2–50.0 µg/mL), Mycobacterium species (MIC = 36.2–125 µg/mL), and Helicobacter pylori (MIC = 25 µg/mL). LCC inhibited the biofilm formation of MSSA and MRSA, demonstrating MBIC50 values of 6.25 μg/mL for both strains. The investigations by fluorescence microscopy, using PI and SYTO9 as fluorophores, indicated that LCC was able to disrupt the S. aureus membrane, similarly to nisin. Systemic toxicity assays using Galleria mellonella larvae showed that LCC was not lethal at 100 µg/mL after 80 h treatment. These data suggest new uses for LCC as a compound with potential applications in antibacterial drug discovery and medical device coating.

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Investigation of Copaifera Genus as a New Source of Antimycobaterial Agents

Cited by 10 publications

References 48 publications

Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption

Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption

Biological Activity of Copaiba in Damage to the Alveolar Bone in a Model of Periodontitis Induced in Rats

Synthesis, Antibacterial Effects, and Toxicity of Licochalcone C

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