Investigation of Immunostimulatory Effects of Heat-Treated
                        Lactiplantibacillus plantarum LM1004 and Its Underlying
                    Molecular Mechanism

Bae, Won-Young; Jung, Woo-Hyun; Shin, So Lim; Kwon, Seulgi; Sohn, Minn; Kim, Tae-Rahk

doi:10.5851/kosfa.2022.e50

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…L. plantarum LM1001 was isolated from kimchi, Korean fermented vegetables, as previously described ( Bae et al, 2022 ). Briefly, homogenized kimchi was diluted in phosphate-buffered saline (PBS) and plated on de Man-Rogosa-Sharpe agar (MRS agar; BD Difco, Franklin Lakes, NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

“…LP-WPC (1%, 2%, and 3%) was treated to C2C12 cells, and the effect on the expression of myogenic regulatory factors, such as myogenic factor 5 protein (Myf-5; Abcam, Cambridge, UK), myoblast determination factor 1 (MyoD; Santa Cruz Biotechnology, Santa Cruz, CA, USA), myogenin (Abcam), was analyzed using western blotting. Cytotoxicity of LP-WPC on C2C12 cells was monitored, and western blotting was conducted, as previously described ( Bae et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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Lactiplantibacillus plantarum LM1001 Improves Digestibility of Branched-Chain Amino Acids in Whey Proteins and Promotes Myogenesis in C2C12 Myotubes

Lee,

So,

Jung

et al. 2024

Food Sci Anim Resour

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Lactiplantibacillus plantarum is a valuable potential probiotic species with various proven health-beneficial effects. L. plantarum LM1001 strain was selected among ten strains of L. plantarum based on proteolytic activity on whey proteins. L. plantarum LM1001 produced higher concentrations of total free amino acids and branched-chain amino acids (Ile, Leu, and Val) than other L. plantarum strains. Treatment of C2C12 myotubes with whey protein culture supernatant (1%, 2% and 3%, v/v) using L. plantarum LM1001 significantly increased the expression of myogenic regulatory factors, such as Myf-5, MyoD, and myogenin, reflecting the promotion of myotubes formation (p<0.05). L. plantarum LM1001 displayed β-galactosidase activity but did not produce harmful β-glucuronidase. Thus, the intake of whey protein together with L. plantarum LM1001 has the potential to aid protein digestion and utilization.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Lactiplantibacillus plantarum LM1001 Improves Digestibility of Branched-Chain Amino Acids in Whey Proteins and Promotes Myogenesis in C2C12 Myotubes

Lee,

So,

Jung

et al. 2024

Food Sci Anim Resour

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“…Cellular fatty acid composition was analyzed using a gas chromatography/mass selective detector (GC/MSD) according to our previous study 12 . GC/MSD system was composed of an Agilent 8890 gas chromatography system with a 5977 B MSD and a 7693A automated liquid sampler (Agilent).…”

Section: Methodsmentioning

confidence: 99%

“…9 Probiotics are wellknown natural antimicrobial agents, 11 although live microorganisms are restricted as cosmetic ingredients because of spoilage. However, postbiotics, which are inactivated probiotics, including lysates and metabolites, 12 show remarkable stability during storage and can be applied to various cosmetics. Therefore, heat-treated P. acidilactici LM1013 (HT-LM1013) was investigated for the inhibition of C. acnes biofilm formation and applied as a leave-on-type product for patients with acne vulgaris.…”

Section: Introductionmentioning

confidence: 99%

Heat‐treated Pediococcus acidilactici LM1013‐mediated inhibition of biofilm formation by Cutibacterium acnes and its application in acne vulgaris: A single‐arm clinical trial

Bae¹,

Jung²,

Lee³

et al. 2023

J of Cosmetic Dermatology

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PurposeAcne vulgaris is a common skin disease accompanied by chronic inflammation in the pilosebaceous follicles, resulting from excessive Cutibacterium acnes. This study aimed to investigate the inhibition of biofilm formation by C. acnes ATCC 6919 using heat‐treated Pediococcus acidilactici LM1013 (HT‐LM1013), previously isolated from the Korean traditional fermented alcoholic beverage—makgeolli, and its application as a leave‐on‐type product for patients with acne vulgaris.MethodsHT‐LM1013 was prepared by Lactomason and homogenized using a high‐pressure homogenizer. The minimum inhibitory concentration (MIC), tricarboxylic acid (TCA) cycle, and lipase activity were evaluated for C. acnes inhibition. Inhibition of biofilm formation was demonstrated using a crystal violet solution. Damaged C. acnes was observed using field‐emission scanning electron microscopy (FE‐SEM). Clinical trials were performed using a leave‐on‐type product containing HT‐LM1013.ResultsHT‐LM1013 inhibited the TCA cycle (36.80%) and lipase activity using palmitate (31.89%), stearate (36.91%), and oleate (30.86%) as substrates at 1 × MIC (p < 0.01). After treatment with HT‐LM1013, concave and elongated shapes of C. acnes were observed by FE‐SEM. In addition, HT‐LM1013 inhibited biofilm formation by 71.75% at 1 × MIC (p < 0.001) and removed 73.35% of mature biofilms (p < 0.01). In the clinical trial, the leave‐on‐type product decreased the number of closed comedones from 14.04 to 10.22, open comedones from 7.22 to 4.39%, and sebum content to 76.23% at week 4 (p < 0.01). The satisfaction score of the participants was recorded 3.83 on a five‐point scale.ConclusionHT‐LM1013 is potent for the treatment of acne vulgaris.

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“…Limosilactobacillus reuteri LM1071 and Streptococcus thermophilus LM1012 have both been reported to have anti-inflammatory effects [ 24 , 25 , 26 ]. Additionally, the immune-boosting effect of a heat-killed material from Lactiplantibacillus plantarum LM1004 has been proven through in vitro and in vivo results, including a human clinical trial [ 27 , 28 , 29 ]. Therefore, the primary goal of the formula was to maintain homeostasis of human immunity with anti-inflammatory and immune-boosting strains.…”

Section: Introductionmentioning

confidence: 99%

Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells

Han¹,

You²,

Cha³

et al. 2023

Microorganisms

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Although leaky gut syndrome is not recognized as an official diagnosis for human diseases, it is now believed that dysfunction of the cell barrier causes increased permeability of intestinal epithelial cells leading to this condition. Probiotics have been widely used to improve gut health, and studies have investigated the relevance of protecting the intestinal barrier by taking probiotic strains in vitro and in vivo. However, most studies have restricted the use of single or several probiotic strains and do not consider commercially available probiotic products composed of multi-species. In this study, we provide experimental evidence that a multi-species probiotic mixture composed of eight different strains and a heat-treated probiotic strain is effective in preventing leaky gut conditions. We employed an in vitro co-culture model system utilizing two different differentiated cell lines to mimic human intestinal tissue. The integrity of epithelial barrier function was protected by the preserving the occludin protein level and activating the AMPK signaling pathway, associated with tight junctions (TJs), through treatment with the probiotic strain mixture in Caco-2 cells. Moreover, we confirmed that application of the multi-species probiotic mixture reduced the expression of proinflammatory cytokine genes by inhibiting NFκB signaling pathway when artificial inflammation was induced in an in vitro co-culture model system. Finally, we proved that the epithelial permeability measured by trans-epithelial electrical resistance (TEER) was significantly decreased in the probiotic mixture treated cells, indicating that the integrity of the epithelial barrier function was not compromised. The multi-species probiotic strain mixture exhibited the protective effect on the integrity of intestinal barrier function via enhancing TJ complexes and reducing inflammatory responses in the human intestinal cells.

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Investigation of Immunostimulatory Effects of Heat-Treated Lactiplantibacillus plantarum LM1004 and Its Underlying Molecular Mechanism

Cited by 8 publications

References 47 publications

Lactiplantibacillus plantarum LM1001 Improves Digestibility of Branched-Chain Amino Acids in Whey Proteins and Promotes Myogenesis in C2C12 Myotubes

Lactiplantibacillus plantarum LM1001 Improves Digestibility of Branched-Chain Amino Acids in Whey Proteins and Promotes Myogenesis in C2C12 Myotubes

Heat‐treated Pediococcus acidilactici LM1013‐mediated inhibition of biofilm formation by Cutibacterium acnes and its application in acne vulgaris: A single‐arm clinical trial

Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells

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