Investigation of in vitro activities of Crocus sativus styles extract relevant to Alzheimer's disease

Παπανδρέου, Μαγδαληνή; Kanakis, C. D.; Polissiou, Moschos G.; Efthimiopoulos, Spiros; Cordopatis, Paul; Margarity, Marigoula; Lamari, F. N.

doi:10.1055/s-2007-987288

Cited by 2 publications

(2 citation statements)

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“…An antidepressant property has been attributed to crocin (Akhondzadeh et al, 2004). It is well known as a potent antioxidant (Ochiai et al, 2006;Papandreou et al, 2006;Zheng et al, 2006), so that it received so much attention against oxidative stress related disorders. In chronically stressed mouse model it prevents impairment of learning and memory as well as lipid peroxidation products.…”

Section: Antiproliferative Effects Of Crocin In Hepg2 Cells By Telomerase Inhibition and Htert Down-regulation Sakineh Kazemi Noureini 1 mentioning

confidence: 99%

Antiproliferative Effects of Crocin in HepG2 Cells by Telomerase Inhibition and hTERT Down-Regulation

Noureini¹,

Wink²

2012

Asian Pacific Journal of Cancer Prevention

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Crocin, the main pigment of Crocus sativus L., has been shown to have antiproliferative effects on cancer cells, but the involved mechanisms are only poor understood. This study focused on probable effect of crocin on the immortality of hepatic cancer cells. Cytotoxicity of crocin (IC 50 3 mg/ml) in hepatocarcinoma HepG2 cells was determined after 48 h by neutral red uptake assay and MTT test. Immortality was investigated through quantification of relative telomerase activity with a quantitative real-time PCR-based telomerase repeat amplification protocol (qTRAP). Telomerase activity in 0.5 μg protein extract of HepG2 cells treated with 3 mg/ml crocin was reduced to about 51% as compared to untreated control cells. Two mechanisms of inhibition, i.e. interaction of crocin with telomeric quadruplex sequences and down regulation of hTERT expression, were examined using FRET analysis to measure melting temperature of a synthetic telomeric oligonucleotide in the presence of crocin and quantitative real-time RT-PCR, respectively. No significant changes were observed in the T m telomeric oligonucleotides, while the relative expression level of the catalytic subunit of telomerase (hTERT) gene showed a 60% decrease as compared to untreated control cells. In conclusion, telomerase activity of HepG2 cells decreases after treatment with crocin, which is probably caused by down-regulation of the expression of the catalytic subunit of the enzyme.

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Section: Antiproliferative Effects Of Crocin In Hepg2 Cells By Telomerase Inhibition and Htert Down-regulation Sakineh Kazemi Noureini 1 mentioning

confidence: 99%

Antiproliferative Effects of Crocin in HepG2 Cells by Telomerase Inhibition and hTERT Down-Regulation

Noureini¹,

Wink²

2012

Asian Pacific Journal of Cancer Prevention

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“…In passive avoidance test, animal's step through latency, as an index for learning, in all test groups was significantly greater than control group. Total time spent in dark room (DS), which opposed the memory retention ability, in AC was significantly greater than AT group at 1 and 2 hours after full training, while there was no significant difference in this parameter between AP and AT [105].…”

Section: Stmentioning

confidence: 88%

Medicinal plants with central nervous effects (part 2): plant based review

Al-Snafi¹

2016

IOSRPHR

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Investigation of in vitro activities of Crocus sativus styles extract relevant to Alzheimer's disease

Cited by 2 publications

References 0 publications

Antiproliferative Effects of Crocin in HepG2 Cells by Telomerase Inhibition and hTERT Down-Regulation

Antiproliferative Effects of Crocin in HepG2 Cells by Telomerase Inhibition and hTERT Down-Regulation

Medicinal plants with central nervous effects (part 2): plant based review

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