Investigation of in vitro and in vivo digestibility of black soldier fly (Hermetia illucens L.) larvae protein

Traksele, Lina; Speičienė, V.; Smicius, Romualdas; Alenčikienė, Gitana; Šalaševičienė, Alvija; Garmienė, Galina; Zigmantaitė, Vilma; Grigalevičiūtė, Ramunė; Kučinskas, Audrius

doi:10.1016/j.jff.2021.104402

Cited by 25 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As for algae, the microalga Chlorella vulgaris has a PDCAAS score of 0.63 [ 36 ], the red alga Palmaria palmata has the highest PDCAAS score of 0.69 in vitro digestive system, while the brown alga Fucus vesiculosus has the lowest PDCAAS score of 0.08 [ 73 ]. The PDCAAS score of insects is close to that of legumes [ 74 , 75 ]. For example, the PDCAAS score of Tenebrio molitor protein obtained from freeze-drying, defatting, extrusion and formic acid hydrolysis is 0.79–0.82 [ 76 ].…”

Section: Nutritional Value Of Meat and Milk Alternative Proteinsmentioning

confidence: 91%

Nutritional Value and Physicochemical Characteristics of Alternative Protein for Meat and Dairy—A Review

Zeng

Chen

Zhang

et al. 2022

Foods

View full text Add to dashboard Cite

In order to alleviate the pressure on environmental resources faced by meat and dairy production and to satisfy the increasing demands of consumers for food safety and health, alternative proteins have drawn considerable attention in the food industry. However, despite the successive reports of alternative protein food, the processing and application foundation of alternative proteins for meat and dairy is still weak. This paper summarizes the nutritional composition and physicochemical characteristics of meat and dairy alternative proteins from four sources: plant proteins, fungal proteins, algal proteins and insect proteins. The difference between these alternative proteins to animal proteins, the effects of their structural features and environmental conditions on their properties, as well as the corresponding mechanism are compared and discussed. Though fungal proteins, algal proteins and insect proteins have shown some advantages over traditional plant proteins, such as the comparable protein content of insect proteins to meat, the better digestibility of fungal proteins and the better foaming properties of algal proteins, there is still a big gap between alternative proteins and meat and dairy proteins. In addition to needing to provide amino acid composition and digestibility similar to animal proteins, alternative proteins also face challenges such as maintaining good solubility and emulsion properties. Their nutritional and physicochemical properties still need thorough investigation, and for commercial application, it is important to develop and optimize industrial technology in alternative protein separation and modification.

show abstract

Section: Nutritional Value Of Meat and Milk Alternative Proteinsmentioning

confidence: 91%

Nutritional Value and Physicochemical Characteristics of Alternative Protein for Meat and Dairy—A Review

Zeng

Chen

Zhang

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…Processing methods like heating will affect the chemical structure of the proteins, which can influence their solubility and render them unavailable for enzymes (Lee et al, 2019;Melgar-Lalanne et al, 2019). Recently, Traksele et al (2021) demonstrated that the protein of dried low-fat BSF larvae was more digestible in an in vitro digestion assay than the protein in larvae that were only dried. The authors of this paper speculate whether this difference in protein digestibility could be due the high amount of fat in the BSF larvae, which can induce an aggregation of the proteins in the larvae, resulting in a limited access of the enzymes to the protein complexes (Traksele et al, 2021).…”

Section: Application Of the Optimised In Vitro Digestion Methods For ...mentioning

confidence: 99%

“…Recently, Traksele et al (2021) demonstrated that the protein of dried low-fat BSF larvae was more digestible in an in vitro digestion assay than the protein in larvae that were only dried. The authors of this paper speculate whether this difference in protein digestibility could be due the high amount of fat in the BSF larvae, which can induce an aggregation of the proteins in the larvae, resulting in a limited access of the enzymes to the protein complexes (Traksele et al, 2021). However, the three batches of BSF meals used in the current study were produced by the same company and underwent the same processing.…”

Section: Application Of the Optimised In Vitro Digestion Methods For ...mentioning

confidence: 99%

In vitro assessment of protein digestibility and mineral solubility of black soldier fly larvae meals for monogastric animals

Silva

Matos

Araujo

et al. 2022

JIFF

View full text Add to dashboard Cite

Animal farming is a fast-growing sector which demands a large supply of feed materials and the search for novel feed ingredients with a smaller impact on the global environment has been intensified. However, before the inclusion of these feed ingredients in animal feed, a preliminary assessment of the quality and performance is needed. This evaluation is usually done by in vivo feeding trials, but in vitro digestion methods have been successfully used for gathering information regarding the nutritional value of the different feed components. Therefore, in this work, an in vitro digestion method was developed using black soldier fly (BSF) larvae meals. The method included seven experimental variables, namely: pH in stomach, pH in intestine, reaction time in stomach, reaction time in intestine, enzyme concentration in stomach, enzyme concentration in intestine and temperature. The experimental variables were screened by means of a fractional factorial design (27-2) that allowed to select the concentration of enzyme in the intestine and temperature (at 7 U and 37 °C, respectively) as the most influential factors on amino acid and mineral solubility in BSF larvae meal. Performing the in vitro digestibility at 37 °C with pH of the stomach and intestine at 2 and 9, reaction time in the stomach and intestine at 1 hour and 4 hours and concentration of enzyme in the stomach and intestine at 1 U and 7 U resulted in the highest overall nutrient solubility. This optimised in vitro digestion methodology was used to assess the nutritional stability of three batches of BSF meals delivered by the same producer. The in vitro digestion method was able to reveal differences between the three batches of BSF meals.

show abstract

“…Hal ini disebabkan karena proses penghilangan lemak yang dilakukan pada maggot hanya sebagian saja sehingga kandungan LK pada maggot yang partially defatted tidak jauh berbeda dengan maggot sebelum defatting, demikian juga dengan kandungan nutrisi lainnya. Namun, bila BSF dipress dengan mesin, kandungan lemak pada maggot berkurang drastis hingga tinggal 20% dari lemak sebelumnya (Traksele et al 2021). Hal ini menyebabkan peningkatan kandungan protein sebesar 2 kali dan nilai kecernaan protein sebesar 1,6 kali pada tepung larva BSF setelah defatting dibandingkan dengan tepung larva BSF tanpa defatting.…”

Section: Kandungan Nutrisi Sumber Protein Pakan Alternatifunclassified

Utilization of Cricket (Gryllus bimaculatus), Black Soldier Fly (Hermetia illucens), Mealworm (Tenebrio molitor), and Silkworm (Samia ricini dan Bombyx mori) as Alternative Feed Protein Sources

Herliatika¹,

Permatahati²,

Pujiawati³

et al. 2021

WARTAZOA

View full text Add to dashboard Cite

<p>The insect which contains high protein is potential to be used as an unconventional protein source (UPS) in feed. Production cost of this feedstuff is affordable through utilization of waste as growing media. Moreover, this production helps the environment by reducing the unprocessed waste. This study aims to review the potential use of cricket, black soldier fly (BSF), mealworm, and silkworm as UPS in replacing fish meal (FM) and soybean meal (SM) in the feed. The insect meal contains relative similar crude protein but higher ether-extract compared to conventional protein sources. The insect meal contains higher tyrosine but lower arginine, histidine, lysine, and tryptophan compared to FM and SM. The chitin content in UPS decreases the digestibility, causing restriction its utilization in the diet. However, it can be used as antibacterial and antifungal in feed, and in ruminant, it can be used to mitigate enteric methane emission. UPS in layer and broiler diet can replace SM, meanwhile, in quail diet, it can replace FM. From different sources, it can be summarised that the potential addition of BSF, cricket, mealworm, and silkworm in the broiler’s diet is 8%DM replacing 100% SM, 5-15%DM replacing 40-100% FM, 5-29,5%DM replacing 3-100% SM, and 7,8%DM replacing 100% SM, respectively. The potential addition of BSF and mealworm in the layer’s diet is 10-15%DM replacing 66-100% SM and 2-5%DM replacing 21% SM, respectively. The potential addition of cricket, mealworm, and silkworm in the quail’s diet is 2-8%DM replacing 25-100% FM, 2,25%DM replacing 25% FM, and 2,08-6,25%DM replacing 26-76% FM, respectively.</p>

show abstract

Investigation of in vitro and in vivo digestibility of black soldier fly (Hermetia illucens L.) larvae protein

Cited by 25 publications

References 25 publications

Nutritional Value and Physicochemical Characteristics of Alternative Protein for Meat and Dairy—A Review

Nutritional Value and Physicochemical Characteristics of Alternative Protein for Meat and Dairy—A Review

In vitro assessment of protein digestibility and mineral solubility of black soldier fly larvae meals for monogastric animals

Utilization of Cricket (Gryllus bimaculatus), Black Soldier Fly (Hermetia illucens), Mealworm (Tenebrio molitor), and Silkworm (Samia ricini dan Bombyx mori) as Alternative Feed Protein Sources

Contact Info

Product

Resources

About