“…In general, relative quantification is a simple and efficient method to assess an assay, but absolute quantification may be more informative due to the ability to interpret results across different assay platforms (Dussault and Pouliot, 2006). Relative quantification of GLUTs and Ki-67 was detected by the Pfaffl method implemented in the fluorescence quantitative PCR detection system (Line-Gene FQD-33A, Bioer, Hangzhou, China) (Gardner, 2010), and the ratio was calculated as follows: …”