Investigation of Leukocyte Viability in A Setup of Spiral Microchannel for Cell Sorting Application

Suwannaphan, Thammawit; Pimpin, Alongkorn; Srituravanich, Werayut; Sailasuta, Achariya; Kaewamatawomg, Theerayuth; Jeamsaksiri, Wutthinan; Sripumkhai, Witsaroot; Piyaviriyakul, Prapruddee; Bhanpattanakul, Suchaya

doi:10.1109/bmeicon.2018.8609967

Cited by 3 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, it is believed that cells in a syringe and needle set-up experience high pressure points at the interface between the syringe and needle, which perhaps, affect cell viability 44 . It is interesting to note that according to these results, this may not be the case, indeed, previous studies have found that the majority of cell death occurs in the channel rather than in the delivery syringe 45 .…”

Section: Viability Of Cancer Cells In Poiseuille Flowmentioning

confidence: 66%

The in vitro inertial positions and viability of cells in suspension under different in vivo flow conditions

Connolly

McGourty

Newport

2020

Sci Rep

View full text Add to dashboard Cite

The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of cell therapy as cells need to be returned to patients' bodies. Despite this, it is unclear how this fundamental fluid regime affects cell viability. This study investigated the influence that varying flow rate, and the corresponding wall shear stress (τ w) has on the viability and inertial positions of circulating cells in laminar pipe flow. The viability of two representative cell lines under different shear stresses in two different systems were investigated while particle streak imaging was used to determine their inertial positions. It was found that peristaltic pumps have a negative effect on cell viability in comparison to syringe pumps. Increasing shear stress in a cone and plate above 3 Pa caused an increase in cell death, however, τ w as high as 10 Pa in circulation has little to no effect on cell viability. Inertial lift forces that move cells towards the centre of the channel protect them from experiencing detrimental levels of τ w , indicating that τ w in Poiseuille flow is not a good predictor of cell viability during advection.

show abstract

Section: Viability Of Cancer Cells In Poiseuille Flowmentioning

confidence: 66%

The in vitro inertial positions and viability of cells in suspension under different in vivo flow conditions

Connolly

McGourty

Newport

2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It has been found, using a syringe and needle set-up, that the viability of primary cells normally suspended in high-velocity fluid flow such as WBCs are unaffected in a 410 µ m inner diameter tube at flow rates of up to 8 mL/min, however, as previously mentioned, cells were only exposed to these conditions for a matter of milliseconds 7,32 . It would, therefore, be interesting to discern if they, similar to breast cancer cells, possess sustained viability over an extended period of time in high-velocity fluid flows.…”

Section: Cell Specific Variation In Viability In Suspension In In Vitromentioning

confidence: 91%

Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

Connolly

Newport

McGourty

2021

Sci Rep

View full text Add to dashboard Cite

The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T-cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes.

show abstract

“…However, achieving cost-effectiveness and reliability for large-scale food production remains a critical challenge. Generally, tissue growth necessitates source cells, a three-dimensional support structure, and a scaffold capable of allowing perfusion for adequate nutrient delivery and waste removal, as well as nutrients, including amino acids and growth factors [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Researchers have successfully prepared cellulose from bacterial sources and decellularized plants, such as spinach, bamboo, algae, apple and Aloe vera. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Aloe vera cellulose-based scaffolds have been used for engineering tissues such as skin and cornea and in regenerative medicine applications such as wound healing [23][24][25][26][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Bovine Lipid Chunks on Aloe vera Scaffolds

Gome,

Chak,

Tawil

et al. 2024

Preprint

View full text Add to dashboard Cite

Aloe vera, renowned for its medicinal and food applications, presents a sustainable, scalable, and cost-effective alternative for cultured meat production. Our innovative method repurposes the Aloe vera parenchyma to produce sterile scaffolds with a conducive porous structure that allows liquid retention. These scaffolds, demonstrating good biocompatibility, support adhesion, proliferation, and extracellular matrix formation of bovine mesenchymal stem cells. The addition of oleic acid leads to the accumulation of lipids, indicating the potential formation of ’lipid chunks’. This cultured bovine 3D tissue, enriched with lipids, can serve as a food additive in plant-based alternative meats, potentially enhancing their texture and flavor profile. To address the scalability challenge, a novel macrofluidic single use bioreactor (MSUB) was used to culture the scaffolds as an example of the potential of various scaling out or up possibilities, enabling sustainable and scalable production of cultured meat or to be used for regenerative medicine for cell-based therapies. This breakthrough in Aloe vera scaffold utilization not only advances the field of tissue engineering but also holds promise for a more sustainable future in alternative protein production.

show abstract

Investigation of Leukocyte Viability in A Setup of Spiral Microchannel for Cell Sorting Application

Cited by 3 publications

References 7 publications

The in vitro inertial positions and viability of cells in suspension under different in vivo flow conditions

The in vitro inertial positions and viability of cells in suspension under different in vivo flow conditions

Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

Cultivation of Bovine Lipid Chunks on Aloe vera Scaffolds

Contact Info

Product

Resources

About