Investigation of Lewis Phenotypes in Polynesians:
Evidence of a Weak Secretor Phenotype

Henry, S.M.; Benny, A.G.; Woodfield, D.G.

doi:10.1159/000461079

Cited by 18 publications

(27 citation statements)

References 10 publications

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“…More recently, the work of Henry ct al. [8] on the Lewis phenotypes in Poly nesians has lent support to the idea of a weak secretor gene Se0 > being responsible for the Le(a+b+) phenotype, a theory also supported by Oriol [pers. commun., 1989].…”

Section: Discussionmentioning

confidence: 82%

The Lewis Blood Group System among Chinese in Taiwan

Broadberry

Lin‐Chu²

1991

Hum Hered

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The nonsecretor gene se is absent (or very rare) among Chinese in Taiwan and the previously reported Le(a+b–) phenotype in this population is in fact Le(a+b+) as proven by the presence of small amounts of Le^b antigen on red blood cells. Salivary ABH substances in this phenotype are usually (although not always) markedly reduced. The Chinese Le(a+b+) phenotype is postulated to be the result of a weak secretor gene Se^ω. Although the Le(a+b+) phenotype is very rare in Caucasians, it has a frequency of 25% in Chinese. All Le(a–b–) Chinese are ABH secretors and have varying amounts of Le^a and/or Le^b substances in saliva.

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Section: Discussionmentioning

confidence: 82%

The Lewis Blood Group System among Chinese in Taiwan

Broadberry

Lin‐Chu²

1991

Hum Hered

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“…In addition, low levels of salivary ABH antigens, that is partial secretion, were found in saliva from Le(aϩbϪ) and Le(aϩbϩ) individuals, suggesting the presence of a weak Se-type ␣1,2-fucosyltransferase (11,12). Molecular analysis of the Se and Sec1 genes of Polynesian people, especially of partial Secretor individuals, will facilitate determination of whether or not RFT-II corresponds to the human Sec1 gene.…”

Section: Discussionmentioning

confidence: 99%

“…Recent molecular cloning of the H and Se genes provided the molecular basis for the Bombay and para-Bombay blood types and the nonSecretor phenotype, respectively, revealing point mutations within the coding regions that abolish the ␣1,2-fucosyltransferase activity (8 -10). On the other hand, the Lewis phenotyping of erythrocytes and secretory glands revealed the Le(aϩbϩ) and partial Secretor phenotypes in selected Polynesian and Asian individuals (11,12). These phenotypes, which are virtually absent in Caucasians, are thought to be caused by weak Se-type ␣1,2-fucosyltransferase activity.…”

mentioning

confidence: 99%

Molecular Cloning and Expression of a Third Type of Rabbit GDP-L-Fucose:β-D-Galactoside 2-α-L-Fucosyltransferase

Hitoshi

Kusunoki

Kanazawa

et al. 1996

Journal of Biological Chemistry

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Recent molecular investigation revealed that two closely related structural genes encode distinct GDP-Lfucose:␤-D-galactoside 2-␣-L-fucosyltransferases (␣1,2-fucosyltransferases). Some human cancer cells or tissues may express an aberrant ␣1,2-fucosyltransferase other than H-and Secretor-type ␣1,2-fucosyltransferase. However, definite evidence of the existence of a third type of ␣1,2-fucosyltransferase has not been demonstrated. Here we report the molecular cloning of a third type of rabbit ␣1,2-fucosyltransferase (RFT-III) from a rabbit genomic DNA library. The DNA sequence included an open reading frame coding for 347 amino acids, and the deduced amino acid sequence of RFT-III showed 59 and 80% identity with those of the previously reported two types of rabbit ␣1,2-fucosyltransferase, RFT-I and RFT-II, respectively. COS-7 cells transfected with the RFT-III gene exhibited ␣1,2-fucosyltransferase activity toward phenyl-␤-Gal as a substrate. Neuro2a (a murine neuroblastoma cell line) cells transfected with the RFT-III gene expressed fucosyl G M1 (type 3 H) but not Ulex europaeus agglutinin-1 lectin reactive antigens (type 2 H). Kinetic studies revealed that RFT-III exhibits higher affinity to types 1 (Gal␤1, 3GlcNAc) and 3 (Gal␤1, 3Gal-NAc) than to type 2 (Gal␤1, 4GlcNAc) oligosaccharides, which suggests that RFT-III as well as RFT-II is a Secretor-type ␣1,2-fucosyltransferase. RFT-III was expressed in the adult gastrointestinal tract. The RFT-I, -II, and -III genes were assigned within 90 kilobases on pulsed field gel electrophoresis analysis. These results constitute direct evidence that, at least in one mammalian species, three active ␣1,2-fucosyltransferases exist.1 catalyzes the fucosylation of terminal ␤-DGal residues and synthesizes H antigens. The activity of ␣1,2-fucosyltransferase was detected in various tissues and body fluids of mammals that had several different kinetic characteristics (1-5). In humans, genetic and biochemical studies have indicated that two distinct but closely linked structural genes (H and Se) code ␣1,2-fucosyltransferases with tissue-specific patterns (1, 6). The human H gene controls the expression of H (Fuc␣1,2Gal␤) antigens (along with A or B antigens or both) on erythrocytes, whereas the Se gene determines the soluble A, B, and H antigens in secretory glands, and Lewis b blood group antigens on red cells (for review, see Ref. 7). Homozygosity for null alleles for the H and Se genes yields the rare Bombay blood type and non-Secretor phenotype, respectively. Recent molecular cloning of the H and Se genes provided the molecular basis for the Bombay and para-Bombay blood types and the nonSecretor phenotype, respectively, revealing point mutations within the coding regions that abolish the ␣1,2-fucosyltransferase activity (8 -10). On the other hand, the Lewis phenotyping of erythrocytes and secretory glands revealed the Le(aϩbϩ) and partial Secretor phenotypes in selected Polynesian and Asian individuals (11, 12). These phenotypes, which are virtually absent in Caucasians, are tho...

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“…The non-secretor phenotype is virtually ab sent in all South American Indians and Orientals [107]. The Le(a+b+) phenotype and partial secretory phenotypes which are absent or rare in most other adult populations [107] are frequent in Polynesians [100,108], Australian abo rigines [109], Chinese [102,110], Japanese [83,103] and Blacks [111],…”

Section: Lewis and Secretor Phenotypesmentioning

confidence: 99%

Lewis Histo-Blood Group System and Associated Secretory Phenotypes

Henry¹,

Oriol²,

Samuelson³

1995

Vox Sanguinis

127

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This review summarises present knowledge of the chemistry, immunology, genetics and clinical significance of antibodies in the Lewis and secretor histo- blood group systems. Although red cell serology has laid the foundations for these systems, more recent advances have been made by studying Lewis and related glycoconjugates with monoclonal antibodies, determining structures by mass spectrometry and NMR spectroscopy, identifying enzymes and their specificities, and identifying the genes by molecular biology. The expression of Lewis system antigens is dependent on Lewis and secretor loci. Fucosyltrans- ferases coded by genes at these loci compete and interact with each other and with other transferases to determine an individual’s Lewis and secretor phenotype. Exocrine epithelial cells, mostly of endodermal origin, synthesise the Lewis antigens which, as plasma glycolipids, are secondarily acquired by cells of the peripheral circulation. Phenotyping red cells is often regarded as a simple way of determining the Lewis and sometimes the secretor status of an individual; however, the red cell phenotype is influenced by many factors and may not necessarily reflect someone’s Lewis and secretor genotypes. Two main red cell Lewis groups are usually found, Lewis negative and Lewis positive. In Lewis-negative individuals, the secretor genotype does not affect the Lewis phenotype, but in Lewis-positive individuals, the non-secretor genotype generates the Le(a+b-) phenotype, the secretor genotype causes the Le(a-b+ ) phenotype, and the partial secretor genotype gives rise to the Le(a+b+) phenotype.

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Investigation of Lewis Phenotypes in Polynesians: Evidence of a Weak Secretor Phenotype

Cited by 18 publications

References 10 publications

The Lewis Blood Group System among Chinese in Taiwan

The Lewis Blood Group System among Chinese in Taiwan

Molecular Cloning and Expression of a Third Type of Rabbit GDP-L-Fucose:β-D-Galactoside 2-α-L-Fucosyltransferase

Lewis Histo-Blood Group System and Associated Secretory Phenotypes

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