Heavy-duty natural gas engines compliant with the 2010 EPA emissions standards have a clear advantage over diesel engines in meeting regulations with minimal after-treatment configuration. Heavy-duty natural gas engines are largely promoted as a cleaner burning engine with respect to low PM mass emissions. However, lack of sufficient data and literature on the exhaust emissions from advanced natural gas engines and the potential adverse health effects has raised concern amongst regulatory agencies. Also, the ammonia emissions from three-way catalyst equipped heavy-duty natural gas engines could be a major contributor to the formation of secondary PM in the atmosphere This CARB funded study focuses primarily on characterizing the toxicity of the volatile fraction of PM from advanced heavy-duty natural gas engines. The objective of the study also includes characterizing the unregulated species of the exhaust together with number concentration and size distribution of ultrafine nanoparticle emissions. CNG fueled transit buses were tested on WVU's heavy-duty chassis dynamometer in Stockton, CA. A wide array of TABLE OF CONTENTS