Investigation of mammalian ovulation with an in vitro perfused rabbit ovary preparation

Wallach, Edward E.; Wright, Karen H.; Hamada, Yasuo

doi:10.1016/s0002-9378(78)80007-6

Cited by 76 publications

(20 citation statements)

References 22 publications

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“…However, this antagonist, like chlorpheniramine, lowered the number of ovulations induced with histamine in the isolated perfused rabbit ovary (Kobayashi et al, 1983). The present experiments on rat ovaries agree with reports from studies on rabbits suggesting that the histamine-induced ovulation involves both receptor subtypes (Wallach et al, 1978;Kobayashi et al, 1983). The H2-receptor antagonism in our experiments was achieved with two kinds of selective antagonists of different chemical nature, cimetidine (Brogden, Heel, Speight & Avery, 1978) and ranitidine (Brogden, Carmine, Heel, Speight & Avery, 1982).…”

Section: Effect Of An H\-antagonistsupporting

confidence: 91%

“…This conforms with observations that the spontaneous contractions of the rabbit ovary, which increase at the time of ovulation, can be completely blocked by the Hrreceptor antagonist, chlorpheniramine (Virutamasen, Wright & Wallach, 1972). Moreover, this antagonist has also been found to reduce hCG-induced ovulations in the rabbit ovary both in vivo (Knox & Beck, 1976) and in vitro (Wallach, Wright & Hamada, 1978). However, the in-vivo effect of chlorpheniramine could not be confirmed by Espey, Stein & Dumitrescu (1982).…”

Section: Effect Of An H\-antagonistmentioning

confidence: 99%

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Histamine induces ovulation in the isolated perfused rat ovary

Schmidt¹,

Owman²,

Sjöberg³

1986

Reproduction

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Summary. Ovulatory effects of histamine and specific antagonists were studied in isolated perfused ovaries from immature rats treated with 10 i.u. PMSG to stimulate follicular growth and maturation. Histamine alone, like LH, induced ovulation in all ovaries tested, but the number of follicular ruptures was lower after histamine (7\m=.\0 and 2\m=.\2 ruptures, respectively, per ovary). The histamine-induced ovulations could be inhibited dose-dependently by the H 1-receptor antagonist, pyrilamine, or the H2\ x=r eq-\ antagonists, cimetidine and ranitidine. At the concentrations tested, these antagonists did not, when given separately, reduce the LH-induced ovulations significantly, but pyrilamine and cimetidine in combination lowered the ovulation frequency by 65%. The prostaglandin synthesis inhibitor, indomethacin, was not able to block the histamine-induced ovulations.

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Section: Effect Of An H\-antagonistsupporting

confidence: 91%

Section: Effect Of An H\-antagonistmentioning

confidence: 99%

Histamine induces ovulation in the isolated perfused rat ovary

Schmidt¹,

Owman²,

Sjöberg³

1986

Reproduction

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“…Prostaglandin E 2 (PGE 2 ), a dominant prostanoid in the ovary, can reverse the inhibitory effect of aspirin-like drugs, when administered simultaneously. These results suggest that some of the steps of ovulation are mediated by this prostanoid (5). Indeed, luteinizing hormone surge leads to high expression of COX-2 in granulosa cells (6,7), and a large amount of PGE 2 is produced and released into the antral fluid (8).…”

mentioning

confidence: 89%

Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP ₂

Hizaki

Segi

Sugimoto

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

448

278

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Female mice lacking the gene encoding the prostaglandin (PG) E 2 receptor subtype EP 2 (EP 2 ؊͞؊ ) become pregnant and deliver their pups at term, but with a much reduced litter size. A decrease in ovulation number and a much reduced fertilization rate were observed in EP 2 ؊͞؊ females without difference of the uterus to support implantation of wild-type embryos. Treatment with gonadotropins induced EP 2 mRNA expression in the cumulus cells of ovarian follicles of wild-type mice. The immature cumuli oophori from wildtype mice expanded in vitro in response to both folliclestimulating hormone and PGE 2 , but the response to PGE 2 was absent in those from EP 2 ؊͞؊ mice. Cumulus expansion proceeded normally in preovulatory follicles but became abortive in a number of ovulated complexes in EP 2 ؊͞؊ mice, indicating that EP 2 is involved in cumulus expansion in the oviduct in vivo. No difference in the fertilization rate between wild-type and EP 2 ؊͞؊ mice was found in in vitro studies using cumulusfree oocytes. These results indicate that PGE 2 cooperates with gonadotropin to complete cumulus expansion for successful fertilization.Ovulation and fertilization are key processes in mammalian female reproduction, which is highly regulated by pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone. These hormones induce a number of preovulatory processes, including follicular development, oocyte maturation, cumulus expansion, and rupture of antral follicles (1). The ovulated eggs move to the oviducts, and timely interaction between an egg and a sperm then leads to successful fertilization (2). Undoubtedly, these processes are initiated by the gonadotropins, but how gonadotropins regulate these processes remains unclear. It is known that some gonadotropin actions are mediated by other mediators. Prostanoids, the cyclooxygenase (COX)-metabolites of arachidonate, likely mediate the ovulatory actions of gonadotropins (3) because aspirin-like drugs that inhibit COX have been reported to inhibit spontaneous and gonadotropin-primed ovulation in many species (4). Prostaglandin E 2 (PGE 2 ), a dominant prostanoid in the ovary, can reverse the inhibitory effect of aspirin-like drugs, when administered simultaneously. These results suggest that some of the steps of ovulation are mediated by this prostanoid (5). Indeed, luteinizing hormone surge leads to high expression of COX-2 in granulosa cells (6, 7), and a large amount of PGE 2 is produced and released into the antral fluid (8). Recently, the importance of prostanoids in early pregnancy has been definitely shown in mice deficient in COX-2. The COX-2 Ϫ͞Ϫ animals showed a reduction in ovulation number and severe failure in fertilization as well as defects in the ability of the uterus to receive implants and to undergo decidualization (9). The result that not only ovulation but fertilization was also severely affected indicates that prostanoids play a role in one of a series of preovulatory processes that are required for both ovulation and fert...

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“…However, PGE2, a dominant prostanoid in the ovary, can reverse the inhibitory effect of aspirin-like drugs (Wallach et al 1978). Additionally, mPGES-1 is preferentially coupled with the inducible COX-2 to promote delayed PGE2 generation .…”

Section: Introductionmentioning

confidence: 99%

Differential expression and regulation of prostaglandin E synthases in the mouse ovary during sexual maturation and luteal development

Sun

Deng²,

Diao³

et al. 2006

Journal of Endocrinology

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Prostaglandin (PGE) 2 is the most common prostanoid and plays an important role in female reproduction. The aim of this study was to examine the expression and regulation of microsomal (m) PGE synthase (PGES)-1 and cytosolic (c) PGES in the mouse ovary during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and immunohistochemistry. Both mPGES-1 mRNA signals and immunostaining were localized in the granulosa cells, but not in the thecal cells and oocytes. cPGES mRNA signals were localized in both granulosa cells and oocytes, whereas cPGES immunostaining was exclusively localized in the oocytes. In our superovulated model of immature mice, there was a basal level of mPGES-1 mRNA signals in the granulosa cells at 48 h after equine chorionic gonadotropin (eCG) treatment. mPGES-1 mRNA level was induced by human chorionic gonadotropin (hCG) treatment for 0·5 h, whereas mPGES-1 immunostaining was slightly induced at 0·5 h after hCG treatment and reached a maximal level at 3 h after hCG treatment. eCG treatment had no obvious effects on either cPGES mRNA signals or immunostaining. A strong level of cPGES immunostaining was present in both unstimulated and eCGtreated groups. Both mPGES-1 mRNA signals and immunostaining were highly detected in the corpus luteum 2 days post-hCG injection and declined from days 3 to 7 post-hCG injection. cPGES immunostaining was at a basal level or not detectable from days 1 to 7 after hCG injection and was highly expressed in the corpus luteum from days 9 to 15 post-hCG injection. PGE2 biosynthesized through the mPGES-1 pathway may be important for follicular development, ovulation and luteal formation.

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Investigation of mammalian ovulation with an in vitro perfused rabbit ovary preparation

Cited by 76 publications

References 22 publications

Histamine induces ovulation in the isolated perfused rat ovary

Histamine induces ovulation in the isolated perfused rat ovary

Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP ₂

Differential expression and regulation of prostaglandin E synthases in the mouse ovary during sexual maturation and luteal development

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Investigation of mammalian ovulation with an in vitro perfused rabbit ovary preparation

Cited by 76 publications

References 22 publications

Histamine induces ovulation in the isolated perfused rat ovary

Histamine induces ovulation in the isolated perfused rat ovary

Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP 2

Differential expression and regulation of prostaglandin E synthases in the mouse ovary during sexual maturation and luteal development

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Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP ₂