Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities

Hong, Jeong Hee; Jang, Seokyoon; Heo, Young Mok; Mao, Min; Lee, Hwanhwi; Lee, Young Min; Lee, Hanbyul; Kim, Jae Jin

doi:10.3390/md13074137

Cited by 79 publications

(68 citation statements)

References 71 publications

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“…Every year, approximately 200 new compounds, including polyketides, alkaloids, sesquiterpenes, and aromatic compounds, are isolated and identified from marine derived fungi (Moghadamtousi, Nikzad, Kadir, Abubakar & Zandi, 2015). Many of them were reported to may have pharmacological potential as antifungal (Hong et al, 2015), antiviral (Moghadamtousi, Nikzad, Kadir, Abubakar & Zandi, 2015), and cytotoxic (Cao et al, 2015). On the other hand, fungi are also known for their pathogenic characteristics.…”

Section: Introductionmentioning

confidence: 99%

Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Fajarningsih

2016

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Despite the fact that fungi are important sources of both bioactive compounds and mycotoxins, and that they are very ubiquitous in our environment, their species identification is hampered by incomplete and often unclear literature. Fungi identification is primarily based on their phenotypic and physiological characteristics. Nowadays, many molecular methods to identify fungal species have been developed. One of the methods considered as a new concept to rapidly and accurately identify unknown fungal sample is DNA Barcoding. This literature review will outline the use of DNA barcoding approach to rapidly identify fungal species and the use of ITS region that recently has been designated as primary DNA barcode for fungal kingdom. "DNA barcode" is a short, highly variable and standardized DNA region with approximately 700 nucleotides in length, which is used as a unique pattern to identify living things. Internal Transcribed Spacer (ITS) region of nuclear DNA (rDNA) has become the most sequenced region to identify fungal taxonomy at species level, and even within species. ITS region is a highly polymorphic noncoding region with enough taxonomic units. Therefore, it is able to separate sequences into species level. Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by few limitations, the ITS will remain as the key choice for fungal identification. The search for alternative regions as DNA marker to improve fungal identification, especially in specific heredities, has already started.

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Section: Introductionmentioning

confidence: 99%

Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Fajarningsih

2016

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…Genomic DNA samples were extracted from dry specimens with the AccuPrep Genomic DNA Extraction Kit (Bioneer, Daejeon, Korea). PCR amplification of the internal transcribed spacer (ITS) region was performed according to the previously described method [5][6][7]. To analyze each species, closely related sequences of Gymnopus spp.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Identification of KoreanGymnopusspp. and the First Report of 3 Species:G. iocephalus,G. polygrammus, andG. subnudus

Jang

Lim

et al. 2016

Mycobiology

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Gymnopus is a cosmopolitan genus of agaric fungi and consists of ~300 species. In Korea, Gymnopus represents common saprobic mushrooms, and 12 species have been reported in Korea. Several Gymnopus specimens were collected in Korea between 2008 and 2015. To identify them exactly, phylogenetic analysis was performed by means of the internal transcribed spacer region of ribosomal-DNA sequences from the collected Gymnopus specimens. Among them, G. iocephalus, G. polygrammus, and G. subnudus have not been reported in Korea. A phylogenetic tree and images are provided.

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“…Hong et al (2015) avaliaram 50 espécies fúngicas de ambiente marinho de diferentes gêneros, cujo estudo determinou que o fungo Arthrinium saccharicola foi o que mostrou melhor capacidade para a produção de celulases (FPAse -Filter Paper Activity 0,39 U/mL) (HONG et al, 2015).…”

Section: Metodologias Usadas Para a Produção De Celulasesunclassified

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

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In Chapter 1 it is reported the cellulase production by 4 marine-derived fungi strains (Aspergillus sydowii CBMAI 934, A. sydowii CBMAI 935, Penicillium citrinum CBMAI 1186 and Mucor racemosus CBMAI 847) and 1 terrestrial fungi strain (Aspergillus sp. CBMAI 1198). They were grown in solid state fermentation using wheat straw as substrate (5 g) and with addition of peptone solution (0,75 g.L -1 ) enriched with inorganic salts. It was performed the enhancement of the growth conditions by changing the temperature, initial pH and moisture. The optimum temperature for all strains varied between 25-35 °C but A. sydowii CBMAI 935 with 40 °C. The optimum pH was different for each strain, varying from acidic to alkaline conditions. The optimum moisture content also varied accordingly the studied strain. In order enhance the cellulose hydrolysis performed by the produced cellulases, it was varied the pH, temperature and amount of the crude cellulase extract during the filter paper hydrolysis reaction. The obtained optimum values were different among strains and, in some cases, there were two optimum pH and temperature for the hydrolysis of the filter paper. Then, the obtained cellulases, using the best conditions for hydrolysis, were used in the sugarcane bagasse hydrolysis (10 FPU/g of sugarcane bagasse). The cellulases from the strains Aspergillus sp. CBMAI 1198 and A. sydowii CBMAI 934 were capable of degrading 75% and 78% of the sugarcane bagasse, respectively, generating reducing sugars. In Chapter 2, the capability of 6 strains (Bacillus subtilis LQOB-SE1, B. coagulans LQOB-SE2, B. pumillus LQOB-SE3, Brevibacillus brevis LQOB-SE4, Lysinibacillus sp. LQOB-SE5 and Serratia marcescens LQOB-SE6), isolated from peat soil, of producing 2,3-butanediol from glycerol fermentation. The only strain that produced 2,3-butanediol was S. marcescens LQOB-SE6, which was also applied in 2,3-butanediol production from glucose fermentation. Therefore, wastes from biodiesel and bioethanol production can be reused in industrial scale. The best conditions for glycerol fermentation: initial pH 7, Nutrient Broth (8 g. Tabela 3. Rendimento e produtividade obtidos de 2,3-butanodiol a partir da fermentação do glicerol pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 182Tabela 4. Comparação entre os valores de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação do glicerol com resultados descritos na literatura para diferentes microrganismos. 183Tabela 5. Rendimentos e produtividades obtidos a partir da fermentação da glicose pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 191Tabela 6. Dados de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação da glicose com microrganismos relatados na literatura e com a bactéria S. marcescens LQOB-SE6.

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Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities

Cited by 79 publications

References 71 publications

Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Phylogenetic Identification of KoreanGymnopusspp. and the First Report of 3 Species:G. iocephalus,G. polygrammus, andG. subnudus

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

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