Investigation of Membrane Proteins in Rat Erythrocytes in Spontaneous Hypertension by Means of Spin-Label Technique

Gulak, Pavel V.; Orlov, Sergei N.; Postnov, Yu. V.; Kiladze, Irina S.; Grigorian, G. L.; Tabagua, Mirab I.; Kajushin, Lev P.

doi:10.1097/00004872-198606000-00009

Cited by 12 publications

(3 citation statements)

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“…Our findings support strongly the concept that impaired filterability in SHR is mainly attributable to altered erythrocyte membrane properties. In fact, abnormal compositions of erythrocyte membrane protein 18,19 and phospholipid 20 have been reported in genetic hypertension of rats, which contributes to reduced erythrocyte membrane fluidity 20 and increased erythrocyte aggregability. 18 It is well known that several types of mechanical stress impair erythrocyte filterability through the action of Ca 2+ -mediated signal transduction; mechanical stress on the erythrocyte membrane causes transmembrane Ca 2+ entry and impairs erythrocyte deformability.…”

Section: Mechanisms Of Impaired Filterabilitymentioning

confidence: 99%

Impaired Erythrocyte Filterability of Spontaneously Hypertensive Rats: Investigation by Nickel Mesh Filtration Technique

Ariyoshi

Maruyama

Odashiro

et al. 2010

Circ J

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Section: Mechanisms Of Impaired Filterabilitymentioning

confidence: 99%

Impaired Erythrocyte Filterability of Spontaneously Hypertensive Rats: Investigation by Nickel Mesh Filtration Technique

Ariyoshi

Maruyama

Odashiro

et al. 2010

Circ J

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“…13 By means of a maleimide spin labeling, Gulak et al reported the different ESR spectra between SHR and Wistar-Kyoto rats (WKY), suggesting that the protein structures in erythrocyte membranes might be altered in hypertension. 14 To gain further insight into membrane abnormalities due to hypertension, we have examined a fatty acid spin-label signal from the erythrocytes of SHR and patients with essential hypertension and compared it to the signal from erythrocytes of normotensive controls. We also investigated calcium-related changes in ESR spectra of erythrocytes by use of calcium ionophore A23187.…”

Section: -20mentioning

confidence: 99%

Electron spin resonance studies of erythrocytes from spontaneously hypertensive rats and humans with essential hypertension.

Tsuda¹,

Iwahashi²,

Minatogawa³

et al. 1987

Hypertension

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SUMMARY The purpose of the present study was to investigate erythrocyte membrane abnormalities in hypertension by means of an electron spin resonance and spin-label technique. The erythrocytes from spontaneously hypertensive rats (SHR) and humans with untreated essential hypertension were examined and compared with their normotensive counterparts, and electron spin resonance spectra were obtained for a fatty spin-label agent (5-nitroxy stearate) incorporated into the erythrocyte membranes. The value of outer hyperfine splitting (2T\) was significantly higher in erythrocytes of SHR and humans with essential hypertension than in erythrocytes of normotensive controls (at 37 °C: SHR, 56.14 ± 0.51 gauss [G], n = 8; Wistar-Kyoto rats, 52.22 ±0.86 G, n = 4, p<0.01; humans with essential hypertension, 56.94 ± 0.27 G, n = 11; normotensive subjects, 55.44 ± 0.36 G, n = 8, p<0.01). The order parameter (5) was also increased in the hypertensive rats and humans compared to their respective normotensive controls. When calcium was loaded to erythrocytes with calcium ionophore A23187 (0.9 fiM) and CaCl 2 (1.0 mM), the parameters of the spectra were increased. These changes were more prominent in the hypertensive groups than in the normotensive controls. These results revealed that the erythrocyte membranes of the hypertensive subjects tolerated different spin motions than those of the normotensive controls in the electron spin resonance study and that membrane fluidity might be decreased in hypertension. Additionally, calcium loading to erythrocytes caused the reduction of membrane fluidity. Therefore, it is suggested that an abnormality of calcium handling at the cellular level might affect physical properties of the biomembranes in hypertension. 1 " 5 It seems likely that generalized abnormalities of the membranes could be involved not only in vascular smooth muscle cells but also in circulating blood cells. Erythrocytes are widely studied because they are a

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“…Numerous studies have shown that the intracellular distribution of monovalent ions in hypertension is correlated with alterations in protein composition of the erythrocyte membrane [14,15,[26][27][28][29][30][31]. Despite incredible advances in genome research, a little concern so far is given to uncovering the pathogenetic link between well characterized genetic determinants of essential hypertension and altered properties of the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Altered erythrocyte membrane protein composition mirrors pleiotropic effects of hypertension susceptibility genes and disease pathogenesis

Polonikov

Ushachev

Иванов

et al. 2015

Journal of Hypertension

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Objective:The study was designed to assess the effects of polymorphisms in genes associated with essential hypertension on the variation of erythrocyte membrane proteins (EMPs) in hypertensive patients. Methods:Major EMPs content was analyzed in blood from 1162 unrelated Russians (235 hypertensive patients, 176 healthy controls, and 751 random individuals from the Central Russia population). Essential hypertension patients were genotyped for 11 polymorphisms of essential hypertension susceptibility, and TGFB1 (rs1800471). EMP contents and their relationship with the genetic loci were analyzed using various statistical tests.Results: Sex-specific differences in EMP contents between the cases and controls were observed. Regardless of sex, hypertensives exhibited mainly decreased levels of alpha (SPTA1) and beta-spectrin (SPTB) and increased levels of glucose transporter (GLUT1) as compared with healthy subjects (P < 0.001). EMP correlated differently in essential hypertension patients and controls. Almost 70% of the joint variation in the EMP levels is explained by five gender-specific principal components. The essential hypertension susceptibility genes showed considerable effects on the levels of spectrins and glucose transporter. A joint variation of the genes explained about half the total polygenic variance in the GLUT1, SPTA1, and SPTB levels in hypertensives. Conclusions:The study showed that essential hypertension susceptibility genes are the important factors of the inherited EMP variation, and their pleitropic effects may be mirrored in the altered expression of genes encoding cytoskeletal proteins and those related to intracellular glucose metabolism.

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Investigation of Membrane Proteins in Rat Erythrocytes in Spontaneous Hypertension by Means of Spin-Label Technique

Cited by 12 publications

References 0 publications

Impaired Erythrocyte Filterability of Spontaneously Hypertensive Rats: Investigation by Nickel Mesh Filtration Technique

Impaired Erythrocyte Filterability of Spontaneously Hypertensive Rats: Investigation by Nickel Mesh Filtration Technique

Electron spin resonance studies of erythrocytes from spontaneously hypertensive rats and humans with essential hypertension.

Altered erythrocyte membrane protein composition mirrors pleiotropic effects of hypertension susceptibility genes and disease pathogenesis

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