Investigation of microbial community structure in an advanced activated sludge side-stream reactor process with alkaline treatment

Yan, Peng; Wang, Jing; Chen, You‐Peng; Ji, Fangying; Shen, Yu; Fang, Fang; Lin, Yi-Hong; Guo, Jinsong; Zhang, Hong; Ouyang, Wenbin

doi:10.1016/j.ibiod.2015.07.003

Cited by 19 publications

(1 citation statement)

References 35 publications

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“…In this study, OTU004 exhibited a negative correlation with pH, which is consistent with a circumneutral optimal growth pH. It has been detected in sewage treatment facilities ( Auguet et al, 2015 ; Yan et al, 2015 ) and coal bed methane environments ( Guo et al, 2012 ), as well as in the deep subsurface ( Ise et al, 2016 ; Jangir et al, 2016 ). In QV1.2A, Group 1 [NiFe]-hydrogenase gene hyaB and the rbcL gene of RuBisCo were detected on contigs identified as Azonexus hydrophilus (Supplementary Dataset S1 ), suggesting that these Betaproteobacteria are involved in H 2 -oxidation and carbon fixation in the moderate wells.…”

Section: Discussionsupporting

confidence: 83%

Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH

et al. 2017

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Serpentinization is a widespread geochemical process associated with aqueous alteration of ultramafic rocks that produces abundant reductants (H2 and CH4) for life to exploit, but also potentially challenging conditions, including high pH, limited availability of terminal electron acceptors, and low concentrations of inorganic carbon. As a consequence, past studies of serpentinites have reported low cellular abundances and limited microbial diversity. Establishment of the Coast Range Ophiolite Microbial Observatory (California, U.S.A.) allowed a comparison of microbial communities and physicochemical parameters directly within serpentinization-influenced subsurface aquifers. Samples collected from seven wells were subjected to a range of analyses, including solute and gas chemistry, microbial diversity by 16S rRNA gene sequencing, and metabolic potential by shotgun metagenomics, in an attempt to elucidate what factors drive microbial activities in serpentinite habitats. This study describes the first comprehensive interdisciplinary analysis of microbial communities in hyperalkaline groundwater directly accessed by boreholes into serpentinite rocks. Several environmental factors, including pH, methane, and carbon monoxide, were strongly associated with the predominant subsurface microbial communities. A single operational taxonomic unit (OTU) of Betaproteobacteria and a few OTUs of Clostridia were the almost exclusive inhabitants of fluids exhibiting the most serpentinized character. Metagenomes from these extreme samples contained abundant sequences encoding proteins associated with hydrogen metabolism, carbon monoxide oxidation, carbon fixation, and acetogenesis. Metabolic pathways encoded by Clostridia and Betaproteobacteria, in particular, are likely to play important roles in the ecosystems of serpentinizing groundwater. These data provide a basis for further biogeochemical studies of key processes in serpentinite subsurface environments.

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Section: Discussionsupporting

confidence: 83%

Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH

et al. 2017

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Enhanced excess sludge hydrolysis and acidification in an activated sludge side-stream reactor process with single-stage sludge alkaline treatment: a pilot scale study

Yan¹,

Guo²,

Wang³

et al. 2016

Environ Sci Pollut Res

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A pilot-scale side-stream reactor process with single-stage sludge alkaline treatment was employed to systematically investigate characteristics of excess sludge hydrolysis and acidification with alkaline treatment and evaluate feasibility of recovering a carbon source (C-source) from excess sludge to enhance nutrient removal at ambient temperature. The resulting C-source and volatile fatty acid specific yields reached 349.19 mg chemical oxygen demand (COD)/g volatile suspended solids (VSS) d and 121.3 mg COD/g VSS d, respectively, the process had excellent C-source recovery potential. The propionic-to-acetic acid ratio of the recovered C-source was 3.0 times that in the influent, which beneficially enhanced biological phosphorus removal. Large populations and varieties of hydrolytic acid producing bacteria cooperated with alkaline treatment to accelerate sludge hydrolysis and acidification. Physicochemical characteristics indicated that recovered C-source was derived primarily from extracellular polymeric substances hydrolysis rather than from cells disruption during alkaline treatment. This study showed that excess sludge as carbon source was successfully recycled by alkaline treatment in the process.

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Industrial wastewaters harbor a unique diversity of bacterial communities revealed by high-throughput amplicon analysis

et al. 2018

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Investigation of microbial community structure in an advanced activated sludge side-stream reactor process with alkaline treatment

Cited by 19 publications

References 35 publications

Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH

Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH

Enhanced excess sludge hydrolysis and acidification in an activated sludge side-stream reactor process with single-stage sludge alkaline treatment: a pilot scale study

Industrial wastewaters harbor a unique diversity of bacterial communities revealed by high-throughput amplicon analysis

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