Investigation of molybdenum-crosslinker interfaces for affinity based electrochemical biosensing applications

Kamakoti, Vikramshankar; Shanmugam, Nandhinee Radha; Tanak, Ambalika Sanjeev; Jagannath, Badrinath; Prasad, Shalini

doi:10.1016/j.apsusc.2017.12.026

Cited by 20 publications

(14 citation statements)

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“…The electrochemical performance of the developed biosensor was captured using non-faradaic EIS technique for the detection of PCT and CRP in human serum and whole blood. EIS is a powerful and sensitive technique which captures the electrochemical interactions at the functionalized electrode surface [ 40 ]. The stable covalent linker binding interaction helps to firmly secure the capture antibody to provide accurate biosensing response.…”

Section: Resultsmentioning

confidence: 99%

Non-faradaic electrochemical impedimetric profiling of procalcitonin and C-reactive protein as a dual marker biosensor for early sepsis detection

Tanak¹,

Jagannath²,

Tamrakar³

et al. 2019

Analytica Chimica Acta: X

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In this work, we demonstrate a robust, dual marker, biosensing strategy for specific and sensitive electrochemical response of Procalcitonin and C-reactive protein in complex body fluids such as human serum and whole blood for the detection of sepsis. Enhanced sensitivity is achieved by leveraging the physicochemical properties of zinc oxide at the electrode-solution interface. Characterization techniques such as SEM, EDAX, AFM, FTIR and fluorescence microscopy were performed to ensure a suitable biosensing surface. The characteristic biomolecular interactions between the target analyte and specific capture probe is quantified through unique frequency signatures using non-faradaic electrochemical impedance spectroscopy (EIS). The developed biosensor demonstrated a detection limit of 0.10 ng mL −1 for PCT in human serum and whole blood with an R 2 of 0.99 and 0.98 respectively. CRP demonstrated a detection limit of 0.10 μg mL −1 in human serum and whole blood with an R 2 of 0.90 and 0.98 respectively. Cross-reactivity analysis demonstrated robust selectivity to PCT and CRP with negligible interaction to non-specific biomolecules. The novel aspect of this technology is the ability to fine-tune individual biomarkers response owing to the optimal frequency tuning capability. The developed biosensor requires an ultra-low sample volume of 10 μL without the need for sample dilution for rapid analysis. We envision the developed dual marker biosensor to be useful as a sepsis-screening device for prognostic monitoring.

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Section: Resultsmentioning

confidence: 99%

Non-faradaic electrochemical impedimetric profiling of procalcitonin and C-reactive protein as a dual marker biosensor for early sepsis detection

Tanak¹,

Jagannath²,

Tamrakar³

et al. 2019

Analytica Chimica Acta: X

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“…114,115 Metal oxide substrates such as hafnium oxide (HfO 2 ), 116,117 aluminium oxide (Al 2 O 3 ), 118 indium tin oxide (ITO) [119][120][121] have been incorporated, more rarely platinum 122,123 and molybdenum. 124 Regarding the transducer surface modication, researchers oen rely on gold nanoparticles (AuNPs) deposition on various substrates to benet from easily addressable gold-sulphur chemistry and the signal amplication due to the accelerated electron transfer between the electrode and a redox moiety/ mediator. 125 Gold-sulphur chemistry is oen explored with selfassembled monolayers (SAMs) based on various thiol derivatives (e.g., MPA, MHDA, MUA) employed to immobilize bioreceptors on gold electrode surface.…”

Section: Design Of Electrochemical Sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 99%

“…113 Some other strategies exploit more peculiar transducer substrates for immobilizing an antibody, such as e.g. molybdenum (via cross-linking with EDC and NHS, employed for detection of CRP) 124 or HfO 2 (via self-assembly, detection of IL-10). 116 Impedimetric detection of mTBI-relevant enzymatic proteins like matrix metalloproteinases (MMPs) is addressed via approaches resting upon a cleavage event of a peptide specic to MMPs aer injection of target-containing solution.…”

Section: Capacitive and Impedimetric (Bio)sensorsmentioning

confidence: 99%

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Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

2021

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“…Schematic of EMAF process, (Ⅰ) the basic machining principle of EMAF process (Ⅱ) the forming mechanism of passive films in ECM process (Ⅲ) the whole process that the workpiece is finished by EMAF process By the way, the forming mechanism of passive films in ECM process as shown in Figure 2 (Ⅱ) [30]. The NaNO3 solution is adopted as the electrolyte in this study.…”

Section: Figurementioning

confidence: 99%

Study on Electrochemical Effects Assisted Magnetic Abrasive Finishing for Finishing Stainless Steel SUS304

Sun

et al. 2021

Preprint

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In order to obtain a high accuracy with high machining efficiency for finishing hard alloy metal material, we proposed a hybrid finishing method which is electrochemical (ECM) effects assisted magnetic abrasive finishing (MAF). In this study, the electrochemical magnetic abrasive finishing (EMAF process) was divided into EMAF stage and MAF stage. The metal surface can be easily finished with the passive films formed in electrochemical reactions. Simultaneously, the passive films can be removed by frictional action between magnetic brush and workpiece surface. Thus, the essence of EMAF process is to form and remove the passive films on the workpiece surface. This study focused on investigating the finishing mechanism and finishing characteristics of EMAF process. Through a series of experimental investigations, it can be confirmed that the finishing efficiency is remarkably improved by EMAF process. The optimal experimental result of EMAF process showed that the surface roughness was reduced to less than 30 nm from the original surface roughness 178 nm at 4 min in EMAF stage, and the surface roughness was finally reduced to 20 nm at 10 min in MAF stage. Additionally, we also found the finishing ability of magnetic abrasive decreased after 4 min EMAF stage.

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Investigation of molybdenum-crosslinker interfaces for affinity based electrochemical biosensing applications

Cited by 20 publications

References 50 publications

Non-faradaic electrochemical impedimetric profiling of procalcitonin and C-reactive protein as a dual marker biosensor for early sepsis detection

Non-faradaic electrochemical impedimetric profiling of procalcitonin and C-reactive protein as a dual marker biosensor for early sepsis detection

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

Study on Electrochemical Effects Assisted Magnetic Abrasive Finishing for Finishing Stainless Steel SUS304

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