Investigation of Morchella esculenta and Morchella conica for their antibacterial potential against methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pyogenes

Haq, Faiz Ul; Imran, Muhammad; Saleem, Sidrah; Aftab, Usman; Ghazal, Ayesha

doi:10.1007/s00203-022-03003-8

Cited by 13 publications

(2 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. esculenta is rich in many health-promoting nutritional components ( Kewlani et al, 2023 ; Vassilev, Denev & Papazov, 2020 ). Current research has highlighted that M. esculenta -derived compounds possess excellent biological activities, including antibacterial ( Haq et al., 2022 ), antioxidant ( Akyuz et al, 2019 ; Bedlovičová et al., 2024 ), and anti-inflammatory ( Zhang et al., 2022a ; Zhang et al., 2022b ). For instance, polysaccharide (Se-MPS) prepared from selenium-enriched M. esculenta could significantly enhance the activity and phagocytosis of macrophages and secrete pro-inflammatory cytokines to exert immunomodulatory functions compared to ordinary morel polysaccharides ( Qian et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

Du,

Huang,

Huang

et al. 2024

PeerJ

View full text Add to dashboard Cite

Although Morchella esculenta (L.) Pers. is an edible and nutritious mushroom with significant selenium (Se)-enriched potential, its biological response to selenium stimuli remains unclear. This study explored the effect of selenium on mushroom growth and the global gene expression profiles of M. esculenta. While 5 µg mL−1selenite treatment slightly promoted mycelia growth and mushroom yield, 10 µg mL−1significantly inhibited growth. Based on comparative transcriptome analysis, samples treated with 5 µg mL−1 and 10 µg mL−1 of Se contained 16,061 (452 upregulated and 15,609 downregulated) and 14,155 differentially expressed genes (DEGs; 800 upregulated and 13,355 downregulated), respectively. Moreover, DEGs were mainly enriched in the cell cycle, meiosis, aminoacyl-tRNA biosynthesis, spliceosome, protein processing in endoplasmic reticulum pathway, and mRNA surveillance pathway in both selenium-treated groups. Among these, MFS substrate transporter and aspartate aminotransferase genes potentially involved in Se metabolism and those linked to redox homeostasis were significantly upregulated, while genes involved in isoflavone biosynthesis and flavonoid metabolism were significantly downregulated. Gene expression levels increased alongside selenite treatment concentration, suggesting that high Se concentrations promoted M. esculenta detoxification. These results can be used to thoroughly explain the potential detoxification and Se enrichment processes in M. esculenta and edible fungi.

show abstract

Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

Du,

Huang,

Huang

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

“…Colonization is the first stage in the pathogenesis of S. aureus infection. Asymptomatic colonized individuals are a source of the human-to-human transmission of this disease ( 3 ). After colonization and invasion of tissue by creating an abscess, this bacterium can cross the host immune system and enter the bloodstream ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

In vivo efficiency of the produced recombinant lysostaphin antimicrobial peptide in treatment of methicillin-resistant Staphylococcus aureus (MRSA) skin infection in a mouse model

Abbasian

Ghaznavi‐Rad

Sadelaji

et al. 2023

IJM

View full text Add to dashboard Cite

Background and Objectives: Staphylococcus simulans secretes an antimicrobial compound called lysostaphin, which has bactericidal properties. It destroys staphylococci through the hydrolysis of peptidoglycan in the cell wall. Therefore, this unique property indicates the high ability of lysostaphin in the treatment of staphylococcal infections and is considered as an anti-staphylococcal agent. Materials and Methods: Escherichia coli BL21 (DE3) competent cells were transformed with pET32a-lysostaphin clone and induced by isopropyl-β-D-thio-galactoside (IPTG). The recombinant protein was purified by affinity chromatography. Recombinant lysostaphin -A-based ointment was used for external wound healing in animal model. In vivo activity of oint- ment was evaluated by clinical evidences and cytological microscopic assessment. Results: Our results showed the recombinant protein was produced exactly. The results of checkerboard tests showed MIC, MBC and antibacterial activity test an acute reduction of cell viability during the use of lysostaphin, and SEM results ap- proved the intense wrecking effects of lysostaphin in combination on bacterial cells. Macroscopic findings and microscopic data showed that the recombinant lysostaphin ointment was effective on excisional wound healing. Conclusion: Our findings proved that the recombinant lysostaphin ointment was effective on wound healing due to Staphy- lococcus aureus infection.

show abstract

Morchella esculenta (L.) Pers.; Morchella steppicola Zerova - MORCHELLACEAE

Gafforov,

İnci,

Rašeta

et al. 2023

Ethnobiology

View full text Add to dashboard Cite

Investigation of Morchella esculenta and Morchella conica for their antibacterial potential against methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pyogenes

Cited by 13 publications

References 72 publications

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

In vivo efficiency of the produced recombinant lysostaphin antimicrobial peptide in treatment of methicillin-resistant Staphylococcus aureus (MRSA) skin infection in a mouse model

Morchella esculenta (L.) Pers.; Morchella steppicola Zerova - MORCHELLACEAE

Contact Info

Product

Resources

About