Investigation of multiple <i>Felis catus</i> papillomavirus types (-1/-2/-3/-4/-5/-6) DNAs in feline oral squamous cell carcinoma: a multicentric study

Abstract

Recent evidence suggests a possible association of Felis catus papillomavirus type 2 (FcaPV-2) DNA with feline oral squamous cell carcinoma (FOSCC). In this study, type-specific PCR targeting two genes (L1/E6 or E1/E6) of FcaPV-1/-2/-3/-4/-5/-6 was performed to detect viral DNA in a large amount of FOSCC samples collected in Italy and Austria. FcaPV-1/-2/-3/-4/-5 were detected in 7/113 (6.2%), 7/93 (7.5%), 6/113 (5.3%), 1/113 (0.9%) and 2/113 (1.8%) specimens, respectively, with different prevalences in Italian vs Austrian samples, whilst FcaPV-6 went undetected. Our results confirms that FcaPV-2 is the most prevalent in FOSCC, followed by FcaPV-1/-3 and suggest that FcaPVs have variable circulation rates in European countries.

Feline Oral Squamous Cell Carcinoma: A Critical Review of Etiologic Factors

“…Altamura and collaborators (2020) [ 35 ], based on the findings, attributed PV an important role in the beginning of the development of SCC, because after the detection of FcaPV-2 in 31.3% of the 31 samples, they detected an expression of E6 and E7 oncogenes in 70% of the infected samples and an association between this with a high viral burden through PCR. Additionally, Altamura and colleagues (2022) [ 37 ] show the high prevalence of FcaPV-1/-2/-3/-4/-5/-6 in samples of FOSCC, confirming that type-2 is the most prevalent. The work of Skor (2015), presented at the Veterinary Cancer Society, also showed a high incidence of PV (12/12).…”

“…The discrepancy in the results obtained by Altamura et al (2020) [ 35 ] in feline samples was attributed to the type of primer (consensus or specific) used in the PCR. The consensus primers amplify the region of the L1 gene of diverse genotypes of PV, some still unknown, while the specific primers have the capacity to detect specific genotypes [ 37 ]. However, the failure in detection of the virus does not seem to be related only to this factor, as some studies have applied both types of primers without detecting the virus in either sample.…”

Possibilities of skin coat color‐dependent risks and risk factors of squamous cell carcinoma and deafness of domestic cats inferred via RNA‐seq data

“…HPV infection is implicated in the development of human squamous cell carcinoma and cervical cancer (Augustin et al, 2020; Bao et al, 2021; Chrysovergis et al, 2021; Hennessey et al, 2009; Husain & Neyaz, 2017; Karina, 2022; Kwon et al, 2022; Teh & Krockenberger, 2021). Feline papillomavirus (FPV) type‐2 has been proposed as the primary cause of squamous cell carcinoma in domestic cats (Altamura et al, 2022; Carrai et al, 2020; Munday & Aberdein, 2012; Munday et al, 2008; Munday et al, 2011; Munday et al, 2017; O'Neill et al, 2011; Teh & Krockenberger, 2021; Thomson et al, 2016; Yamashita‐Kawanishi et al, 2021). HPV and FPV are distinct viruses; however, their E5 , E6 , and E7 oncogenes exhibit similar features (Altamura et al, 2016; Jiang & Jiang, 2014; Karina, 2022).…”

Possibilities of skin coat color-dependent risks and risk factors of squamous cell carcinoma and deafness of domestic cats inferred via RNA-seq data

“… Pathways in cancer (FDR ∼ 3.58×10 -3 ) The p53 signaling pathway (FDR ∼ 0.013×10 -2 ) Human papillomavirus (HPV) infection (FDR ∼ 8.33 × 10 -2 ) HPV infection is implicated in the development of human squamous cell carcinoma and cervical cancer (Hennessey et al, 2009; Husain et al, 2017; Augustin et al, 2020; Bao et al, 2021; Chrysovergis et al, 2021; The et al, 2021; Karina, 2022; Kwon et al, 2022). Feline papillomavirus (FPV) type-2 has been proposed as the primary cause of squamous cell carcinoma in domestic cats (Munday et al, 2008; Munday et al, 2011; O’Neill et al, 2011; Munday et al, 2012; Thomson et al, 2016; Munday et al, 2017; Carrai et al, 2020; Yamashita-Kawanishi et al, 2021; Teh et al, 2021; Altamura et al, 2022). HPV and FPV are distinct viruses; however, their E5 , E6 , and E7 oncogenes exhibit similar features (Jiang et al, 2014; Altamura et al, 2016; Karina, 2022).…”