The spatial profile of neutral hydrogen temperatures in Aditya-U tokamak plasma has been estimated from the spatial profile of the Hα spectral emissions measured using a high-resolution multi-track spectrometer, having a spectral resolution of 0.023 nm at a 50 μm entrance slit width. The neutral temperature estimation from the Doppler broadened spectral line was carried out after considering the Zeeman effect due to the magnetic field present in the tokamak. To accurately obtain the temperature of the neutral hydrogen, two temperature components (warm and hot) were required to be considered. A code was developed to obtain the neutral temperature and is used to analyze two typical plasma discharges. The temperature of warm components varies between 3 and 5 eV, while hot atoms have temperatures in the range of 15–30 eV. It was observed that the chord-integrated neutral temperature increases slightly towards the plasma core region compared to the plasma edge of Aditya-U tokamak.