Due
to the difficulty
of sampling, the characteristics of biofilm
communities in drinking water mains are still poorly understood. In
this study, 13 water mains were collected to investigate the occurrence
of opportunistic pathogens, to explore the biofilm community structures,
and to decipher the mechanisms of biofilm community assembly. The
results showed high frequencies of detection of Legionella
pneumophila, Acanthamoeba, and Vermamoeba
vermiformis. The number of mycobacterial gene copies in ductile
iron pipes (DIPs) was significantly higher than that in cast iron
pipes (CIPs), indicating that pipe materials affected colonization
by
opportunistic pathogens. The biofilm samples were clearly clustered
into two groups by pipe materials, highlighting the critical role
of the pipe material in shaping biofilm communities. Furthermore,
nitrifying bacteria were dominant in DIPs, while denitrifying bacteria
were more abundant in CIPs than in DIPs. Overall, this work provides
ecological insights into a poorly understood ecosystem.