Investigation of novel putative immunogenic targets against Staphylococcus aureus using a reverse vaccinology strategy

Goodarzi, Narjes Noori; Bolourchi, Negin; Fereshteh, Sepideh; Shirazi, Armaghan Soltani; Pourmand, Mohammad Reza; Badmasti, Farzad

doi:10.1016/j.meegid.2021.105149

Cited by 8 publications

(7 citation statements)

References 46 publications

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“…The previous study on MRSA 252 genome revealed that IsaA, HlgA, SsaA, and IsdB were the best immunogenic targets [ 45 ]. In the present study, the 20 proteins were localized in secreted (SEC), surface-exposed (PSE), and membrane (MEM) regions.…”

Section: Resultsmentioning

confidence: 99%

Identification of Putative Vaccine and Drug Targets against the Methicillin-Resistant Staphylococcus aureus by Reverse Vaccinology and Subtractive Genomics Approaches

et al. 2022

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Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen and responsible for causing life-threatening infections. The emergence of hypervirulent and multidrug-resistant (MDR) S. aureus strains led to challenging issues in antibiotic therapy. Consequently, the morbidity and mortality rates caused by S. aureus infections have a substantial impact on health concerns. The current worldwide prevalence of MRSA infections highlights the need for long-lasting preventive measures and strategies. Unfortunately, effective measures are limited. In this study, we focus on the identification of vaccine candidates and drug target proteins against the 16 strains of MRSA using reverse vaccinology and subtractive genomics approaches. Using the reverse vaccinology approach, 4 putative antigenic proteins were identified; among these, PrsA and EssA proteins were found to be more promising vaccine candidates. We applied a molecular docking approach of selected 8 drug target proteins with the drug-like molecules, revealing that the ZINC4235426 as potential drug molecule with favorable interactions with the target active site residues of 5 drug target proteins viz., biotin protein ligase, HPr kinase/phosphorylase, thymidylate kinase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, and pantothenate synthetase. Thus, the identified proteins can be used for further rational drug or vaccine design to identify novel therapeutic agents for the treatment of multidrug-resistant staphylococcal infection.

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Section: Resultsmentioning

confidence: 99%

Identification of Putative Vaccine and Drug Targets against the Methicillin-Resistant Staphylococcus aureus by Reverse Vaccinology and Subtractive Genomics Approaches

et al. 2022

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“…Reverse vaccinology has been used as a promising strategy for increasing the chances of finding an applicable target for vaccines against infectious diseases. The majority of the studies have been performed using bacterial genomes such as Mycoplasma pneumoniae [ 68 ], Shigella dysenteriae [ 69 ] and Staphylococcus aureus [ 70 ], but it has also been applied for viruses and fungi, such as Ebola [ 71 ] and Candida tropicalis [ 72 ], respectively. This bioinformatics approach has not only been used in order to increase the probability of finding a potential vaccine candidate, but also for the search of targets for new drugs.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets

Almeida

Roque

Felice

et al. 2023

JoF

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Histoplasma capsulatum is a thermodymorphic fungus that causes histoplasmosis, a systemic mycosis that presents different clinical manifestations, ranging from self-limiting to acute lung infection, chronic lung infection and disseminated infection. Usually, it affects severely immunocompromised patients although immunocompetent patients can also be infected. Currently, there are no vaccines to prevent histoplasmosis and the available antifungal treatment presents moderate to high toxicity. Additionally, there are few options of antifungal drugs. Thus, the aim of this study was to predict possible protein targets for the construction of potential vaccine candidates and predict potential drug targets against H. capsulatum. Whole genome sequences from four previously published H. capsulatum strains were analyzed and submitted to different bioinformatic approaches such as reverse vaccinology and subtractive genomics. A total of four proteins were characterized as good protein candidates (vaccine antigens) for vaccine development, three of which are membrane-bound and one is secreted. In addition, it was possible to predict four cytoplasmic proteins which were classified as good protein candidates and, through molecular docking performed for each identified target, we found four natural compounds that showed favorable interactions with our target proteins. Our study can help in the development of potential vaccines and new drugs that can change the current scenario of the treatment and prevention of histoplasmosis.

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“…Many previous studies have detected the presence of bbp gene in Gram-positive bacteria, especially in S. aureus whether methicillin or vancomycin-resistant [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of bone sialoprotein-binding protein gene among clinical vancomycin-resistant Enterococcus faecium isolated from bone infections

Al-Alak,

Muhammed,

Khazaal

2023

Reviews and Research in Medical Microbiology

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Vancomycin resistant Enterococcus faecium can be found in the bone resulting human osteomyelitis infections, this could be controlled by some encoding genes that can express the bacterial adhesions, which aid in bacterial colonization for the host bone tissues. Hence, this research aimed to detect the occurrence of bbp gene in clinical vancomycin resistant E. faecium isolated from bone infections. After collection, the samples were isolated and identified using different manual and automated protocols. Furthermore, vanA and bbp genes were molecularly investigated. Results show that 60% of the identified E. faecium were vancomycin-resistant with distribution of 32% and 28% in postsurgery and hematogenous infections, respectively. The prevalence of vanA and bbp genes was 100% in 20 vavancomycin-resistant faecium isolates. These findings show that vanA and bbp expression may be associated with bone infections. We draw the conclusion from this study that vanA and bbp detection can be used as a marker for vavancomycin-resistant faecium in the bone invasion.

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Investigation of novel putative immunogenic targets against Staphylococcus aureus using a reverse vaccinology strategy

Cited by 8 publications

References 46 publications

Identification of Putative Vaccine and Drug Targets against the Methicillin-Resistant Staphylococcus aureus by Reverse Vaccinology and Subtractive Genomics Approaches

Identification of Putative Vaccine and Drug Targets against the Methicillin-Resistant Staphylococcus aureus by Reverse Vaccinology and Subtractive Genomics Approaches

Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets

Occurrence of bone sialoprotein-binding protein gene among clinical vancomycin-resistant Enterococcus faecium isolated from bone infections

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