Investigation of osteoblast cells behavior in polymeric 3D micropatterned scaffolds using digital holographic microscopy

Abstract

The effect of micropatterned polymeric scaffolds on the features of the cultured cells at different time intervals after seeding was investigated by digital holographic microscopy. Both parallel and perpendicular walls, with different heights, were fabricated using two-photon lithography on photopolymers. The walls were subsequently coated with polypyrrole-based thin films using the matrix assisted pulsed laser evaporation technique. Osteoblast-like cells, MG-63 line, were cultured on these polymeric 3D micropatterned scaffolds. To analyze these scaffolds with/without cultured cells, an inverted digital holographic microscope, which provides 3D images, was used. Information about the samples' refractive indices and heights was obtained from the phase shift introduced in the optical path. Characteristics of cell adhesion, alignment, orientation, and morphology as a function of the wall heights and time from seeding were highlighted.

Spatiotemporal control of cardiac anisotropy using dynamic nanotopographic cues

“…To address this issue, digital holographic microscopy was employed to obtain 3D dynamic imagery of surface topography during the shape-memory transition. Holographic microscopy is capable of providing quantitative marker-free imaging under usual laboratory conditions [31, 33]. Although the z-axis resolution is sufficiently high to monitor the change of nanoscale transition, the direct observation of nanotopography with 400_500_150 nm (ridge width_groove width_depth) is made difficult by the reliance of holographic microscopy on standard optical microscope magnifications.…”

Laser Processed Antimicrobial Nanocomposite Based on Polyaniline Grafted Lignin Loaded with Gentamicin-Functionalized Magnetite

“…The in vitro cytotoxicity evaluation of the PANI-LIG-based thin coatings was done for osteoblast-like cells (MG-63) (ATCC, Manassas, VA, USA) [39] and [40]. Samples were sterilized by UV exposure during 1 h and washed with complete culture medium (Eagle’s MEM- Biochrom, supplemented with 10% FBS, 1% L-glutamine, 1% P/S, and 1% NEAA at 37 °C) during 10 min.…”

Three-Dimensional Conductive Scaffolds as Neural Prostheses Based on Carbon Nanotubes and Polypyrrole

“…21 Such versatility offers a wide range of options to modify their chemical, biological and physical properties, thus becoming very 5 important materials for biosensors, neural prostheses, drug delivery devices and tissue engineering scaffolds. [22][23] Polypyrrole (PPy) is among the most used and studied CPs for bio-applications, [24][25] although, as common in most CPs, its non-soluble nature in water only allows PPy to be processed as coatings or doped with stabilizing agents, such as PSS. Its conductive nature and high biocompatibility make it an outstanding material to develop scaffolds not only to be used as electrodes for recording or stimulating cell behavior, but also for an improved cell growth without any external stimulus.…”

“…Polypyrrole (PPy) is among the most used and studied CPs for bioapplications, , although, as common in most CPs, its non-soluble nature in water only allows PPy to be processed as coatings or doped with stabilizing agents, such as PSS. Its conductive nature and high biocompatibility make it an outstanding material to develop scaffolds not only to be used as electrodes for recording or stimulating cell behavior but also for an improved cell growth without any external stimulus. ,, PPy has been used to provide electrical properties to non-conductive 3D structures, by coating polymeric films onto previously synthesized non-conductive electrospun fibers, such as collagen or PLLA. − However, very little is reported about the combination of PPy and CNTs, being most of the works related to the development of 2D films. ,− To the best of our knowledge, a tridimensional structure purely composed of PPy and CNT has never been synthesized before.…”