Investigation of persistent infection of bovine viral diarrhea virus (BVDV) in Holstein dairy cows

Garoussi, Massoud Talebkhan; Mehrzad, Jalil; Nejati, Ahmad

doi:10.1007/s11250-018-1765-6

Cited by 12 publications

(14 citation statements)

References 26 publications

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“…The presence of antibodies specific to BVDV is the first method used to detect the presence of PI animals and is the first step for diagnoses of the herds and controlling the spread of this virus in the region without the use vaccination [21,22]. Subsequently, other methods are used to confirm this, and the pursuit of further studies is used to identify this and to remove such infected animals as soon as possible [23]. It is important for control BVDV because this infection is considered to be distributed worldwide in farm animals, and evidence for the natural susceptibility of wildlife species [24,25] comes mainly from serological surveys.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of BVDV Infection and the Presence of Potential Risk Factors in Dairy Cattle Herds in Poland

Rypuła

Płoneczka-Janeczko

Czopowicz

et al. 2020

Animals

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The aim of the study was to analyze the risk factors of BVDV infection, in different regions of Poland, with respect to certain parameters of animal health, including productivity, herd management practices, the presence of BVDV, and the effect of non-vaccination. A total of 354 cattle herds were estimated and linked to the analysis of the different factors that might be correlated with productive results. The presence of antibodies against BVDV was found in 33.3% of examined herds, and the heterogenous distribution of BVDV-positive herds in all regions of Poland (p = 0.001) was confirmed. We found statistical correlations between BVDV infection and pasture (p = 0.004) and the number of grazing animals (p < 0.001), and also the purchase of animals for replacement (p = 0.004) was observed. Production or clear clinical outcomes potentially linked to BVDV infection in the herd have not been observed. The results of this study indicate that the rate of BVDV infection was most strongly correlated with the geographical location of the examined cattle populations in Poland. The second most significant factors were the grazing of animals and the purchasing of new animals to be introduced to herds. The strengthened procedures of management, as well as the implementation of eradication programs, should be considered in the examined herds.

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Section: Discussionmentioning

confidence: 99%

Occurrence of BVDV Infection and the Presence of Potential Risk Factors in Dairy Cattle Herds in Poland

Rypuła

Płoneczka-Janeczko

Czopowicz

et al. 2020

Animals

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“…The disease results in multiple clinical symptoms in the respiratory system, digestive tract, and reproductive system (3)(4)(5). In addition, recessive infections of BVDV result in consequences of abortion, which is a key feature in the epidemiology of this disease (6). Thus, control options for reducing BVDV infections were based on initial screening for serological evidence of persistently infected animals (7).…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus

Yao

Wang

et al. 2021

Front. Vet. Sci.

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Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting virus RNA. In this study, the expression and purification of LwCas13a protein was optimized and the RNase activity of LwCas13a in vitro was verified. CRISPR-LwCas13a system could detect BVDV virus and BVDV RNA with high specificity and simplicity. The detection limit of the LwCas13a system was 103 pM, and there were no cross-reactions with HEK293T and MDBK. In summary, a sensitive, specific, and simple nucleic acid detection method based on CRISPR-Cas13a was developed for BVDV. This method provides a new detection strategy for early diagnosis of BVDV.

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“…Infection of cows with BVDV during early pregnancy (the days 30-120) may lead to the birth of persistently infected (PI) calves. PI animals are significant because they remain viremic throughout their lives and serve as infection sources in cattle herds [4,5]. BVDV is highly essential economically, as the mean annual loss directly attributed to BVDV is estimated to be 42.14€ per livestock [6].…”

Section: Introductionmentioning

confidence: 99%

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

2021

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Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5′ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

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Investigation of persistent infection of bovine viral diarrhea virus (BVDV) in Holstein dairy cows

Cited by 12 publications

References 26 publications

Occurrence of BVDV Infection and the Presence of Potential Risk Factors in Dairy Cattle Herds in Poland

Occurrence of BVDV Infection and the Presence of Potential Risk Factors in Dairy Cattle Herds in Poland

CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

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