Investigation of phenolic compounds at the leaves and shoots Arctostaphylos spp. and their antioxidant and antityrosinase activities

Shamilov, A.A.; Оленников, Д. Н.; Поздняков, Д. И.; Бубенчикова, В. Н.; Garsiya, Ekaterina Robertovna

doi:10.1080/14786419.2021.2025370

Natural Product Research

2022

DOI: 10.1080/14786419.2021.2025370

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Investigation of phenolic compounds at the leaves and shoots Arctostaphylos spp. and their antioxidant and antityrosinase activities

A.A. Shamilov

Д. Н. Оленников

Д. И. Поздняков

et al.

Abstract: Comparative phytochemical and pharmacological analysis of leaves and shoots of Arctostaphylosuva-ursi (L.) Spreng. and endemic of Transcaucasia and North Caucasia ArctostaphyloscaucasicaLipsch. was carried out. Phenolic compounds in methanol extracts were investigated by HPLC-PDA-ESI-MS.Pharmacological investigation was carried out at the models of generation of superoxide, DPPH, hydroxyl and nitrosyl radicals. The antityrosinase properties were evaluated in the reaction of tyrosinase inhibition using L -tyros… Show more

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Cited by 4 publications

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The component composition of the total flavonoid fractions from some Ericaceae family and their effect on the NO-stimulating activity of peritoneal macrophages

Reshetov,

Ligacheva,

Trofimova

et al. 2024

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Representatives of the Ericaceae family are quite common in Russia and are promising for the creation of new medicines of plant origin. At the same time, only 4 of them are official. The study of biologically active substances and pharmacological activity of Andromeda polifolia L., Chamaеdaphne calyculata (L.) Moench, Ledum palustre L., Empetrum nigrum L. with rich resource reserves is promising.Aim. Comparative study of the composition of total flavonoid fractions from A. polifolia, C. calyculata, L. palustre, E. nigrum and study of their effect on the NO-stimulating activity of peritoneal macrophagesMaterials and methods. The crushed aboveground part (leafy shoots) was previously depigmented with chloroform, treated with 70 % aqueous acetone, then acetone was removed. Flavonoids were extracted with ethyl acetate from the aqueous phase. The identification of flavonoids was carried out by HPLC (UltiMate 3000 chromatograph) according to the coincidence of retention times and spectral characteristics, the calculation of the content was carried out by simple normalization. The effect of the samples on nitric oxide production was studied on macrophages of C57BL/6 mice. Endotoxin control in the samples was carried out using a LAL test and cell incubation in the presence of polymyxin B.Results and discussion. The composition of flavonoid fractions from A. polifolia, C. calyculata, L. palustre and E. nigrum has been studied. 8 phenolic compounds were found in C. calyculata shoots, including isoquercitrin, herbacetin, naringenin and naringin – for the first time for this species. 5 compounds were detected in A. polifolia shoots, including isoquercitrin and herbacetin, for the first time for this species. 5 and 4 compounds were identified in L. palustre and E. nigrum shoots, respectively, while quercetin glycosides are predominant in all samples: isoquercitrin, hyperoside and rutin. The fraction of C. calyculata flavonoids at doses of 1, 5, 10 mcg/ml inhibits the production of nitric oxide by macrophages by 30 %, and E. nigrum flavonoids at doses of 100 and 200 mcg/ml, on the contrary, enhance the production of nitrites by macrophages by 33 and 37 %, respectively.Conclusion. A comparative study of the composition of total flavonoid fractions from A. polifolia, C. calyculata, L. palustre, and E. nigrum, which are capable of activating both M1 and M2 polarization of peritoneal macrophages in mice, has been conducted, which requires further in-depth study. The flavonoids C. calyculata and E. nigrum are promising for further study.

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The component composition of the total flavonoid fractions from some Ericaceae family and their effect on the NO-stimulating activity of peritoneal macrophages

Reshetov,

Ligacheva,

Trofimova

et al. 2024

Razrabotka i registraciâ lekarstvennyh sredstv

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Chemical Constituents, Neuroprotective and Antioxidant Potential of Klasea centauroides Leaves

Razuvaeva

Markova²,

Торопова³

et al. 2023

Applied Sciences

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Owing to progressive aging in the population, there is an increase in patients with cognitive impairment. For the prevention of dementia, the use of plant remedies is relevant. Of particular interest is Klasea centauroides (L.) Cass. (Serratula centauroides L., Asteraceae), which has significant natural reserves, contains a wide range of biologically active substances, and is used in folk medicine to treat nervous system diseases. This study aimed to estimate the neuroprotective, energy-protective, and antioxidant effects of K. centauroides extract in cholinergic deficiency caused by long-term scopolamine administration. It has been established that K. centauroides extract accelerates passive avoidance-conditioned reflex development and ensures its preservation over a longer time period under cholinergic deficiency conditions. The K. centauroides extract increases the resistance of brain tissues to the toxic effects of scopolamine, reducing the number of neuron regressive forms in the cerebral cortex and hippocampus. The K. centauroides extract enhances the predominance of aerobic glycolysis over anaerobic glycolysis and enhances the NADH-dehydrogenase and succinate-dehydrogenase complexes activity, thus promoting more intensive ATP synthesis against this background, the introduction of scopolamine. The use of K. centauroides extracts reduces the malonic dialdehyde (MDA) content in the brain structures and increases the catalase (CAT) and antioxidant system glutathione unit activities.

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Caucasian Blueberry: Comparative Study of Phenolic Compounds and Neuroprotective and Antioxidant Potential of Vaccinium myrtillus and Vaccinium arctostaphylos Leaves

Shamilov

Оленников

Поздняков

et al. 2022

Life

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(1) Background: Two Caucasian blueberries Vaccinium myrtillus L. and Vaccinium arctostaphylos L. are famous berry bushes growing in the Caucasus region and used to treat neurological diseases, but the chemistry and bioactivity of leaf extracts are still poorly studied. (2) Methods: Phenolic compounds of V. myrtillus and V. arctostaphylos leaf extracts were profiled and quantified by HPLC–PDA–ESI–tQ–MS. The neurotropic potential of Vaccinium extracts was studied using the model of middle cerebral artery permanent occlusion to determine cerebral blood flow, the area of the brain tissue necrosis, and antioxidant enzyme activity (including superoxide dismutase, succinate dehydrogenase, and cytochrome C oxidase), as well as the concentration of TBARS. (3) Results: Hydroxycinnamates and flavonoids were identified in the leaves of V. myrtillus and V. arctostaphylos, and the dominant metabolite of both extracts was 5-O-caffeoylquinic acid in the amount of 105–226 mg/g. The studied extracts enhanced the cerebral hemodynamics and decreased the frequency of necrotic and lipooxidative processes in the brain tissue, accompanied by an increase in the activity of antioxidant enzymes. The positive effect of V. arctostaphylos was stronger and exceeded the effectiveness of Ginkgo biloba standardized extract. (4) Conclusion: The leaf extracts of Caucasian blueberries V. myrtillus and V. arctostaphylos as a new source of hydroxycinnamates demonstrated a protective effect of the brain ischemia pathology and can be used as therapeutic agents to treat neurological diseases.

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Investigation of phenolic compounds at the leaves and shoots Arctostaphylos spp. and their antioxidant and antityrosinase activities

Cited by 4 publications

References 31 publications

The component composition of the total flavonoid fractions from some <i>Ericaceae</i> family and their effect on the NO-stimulating activity of peritoneal macrophages

The component composition of the total flavonoid fractions from some <i>Ericaceae</i> family and their effect on the NO-stimulating activity of peritoneal macrophages

Chemical Constituents, Neuroprotective and Antioxidant Potential of Klasea centauroides Leaves

Caucasian Blueberry: Comparative Study of Phenolic Compounds and Neuroprotective and Antioxidant Potential of Vaccinium myrtillus and Vaccinium arctostaphylos Leaves

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