Investigation of Polyphenol Composition, Biological Activities and Detoxification Properties of Some Medicinal Mushrooms from Turkey

Abstract

Objectives: Ethanolic extracts of Ganoderma adsperum, Inonotus hispidus, Russula chloroides and Sarcodon imbricatus mushroom species were investigated for their polyphenolic contents and biological activities. Materials and Methods: The radical scavenging activity of the extracts were evaluated by using DPPH 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) method and their polyphenolic compounds were determined by HPLC analysis. Furthermore, the activity effects of mushroom extracts on the GST (glutathione-S-transferase) enzyme were also examined. Additionally, antimicrobial activity of mushroom extracts were evaluated with disc diffusion method. Results: Ethanolic extract of I. hispidus demonstrated the highest total phenolic content and total flavonoid contents with 227.23 ± 4.96 mg gallic acid equivalent/g and 42.14 ± 0.20 quercetin equivalent/g values, respectively. The highest DPPH radical scavenging activity was also observed for ethanolic extracts of I. hispidus with 10.687 ± 1.643 µg/mL IC50 value. HPLC analysis demonstrated that R. Chloroides was comprised of ferulic acid, gallic acid and myricetin compounds. The highest GST enzyme activity effect was detected by the ethanol extracts of I. hispidus and S. u n c o r r e c t e d p r o o f imbricatus. None of the mushroom extracts demonstrated significant inhibition on the bacteria strains used. Conclusion: These results indicate that I. hispidus may be proposed as a new potential source of natural medicine and its potential may be related to its polyphenolic content, which needs further investigation.

Chemical compositions and antioxidant activities of four different mushroom species collected from Turkey

“…It is also reported in the literature that the total phenolic and flavonoid content of S. imbricatus is 1.50-13.20 and 1.46-5.45 mg/g, respectively (Barros et al, 2007a,b;Shomali et al, 2019) and contains gallic acid (0.75 mg/g) and myrcetin (2.89 mg/g) (Shomali et al, 2019). It is seen that the total phenolic/flavonoid compound data obtained from the present study differ from the literature data.…”

“…In a study conducted by Barros et al (2017c), it was reported that cooking and processing processes reduced the effectiveness of S. imbricatus in the -carotene bleaching test. In other studies on the DPPH radical scavenging activity of S. imbricatus, it has been reported that methanol extract exhibited remarkable efficacy (Marcotullio et al, 2008;Luo et al, 2017;Shomali et al, 2019).…”

Submerged cultivation and phytochemical analysis of medicinal mushrooms (Trametes sp.)

“…However, ethanol extraction was discovered to be the most effective method for extracting phytochemicals and polysaccharides from the biomass of mushrooms and their fruiting bodies. Shomali et al (2019) examined the polyphenolic contents and biological activities of four wild mushrooms and found that the ethanolic extracts of I. hispidus had the highest total phenolic and flavonoid contents when compared to other mushroom species. Our results also showed high b-glucan, total phenolic, flavonoid, saponin, and Recently, research has focused on the many health benefits of dietary fibers with macrofungal origins.…”