Investigation of presence of endofungal bacteria in Rhizopus spp. ısolated from the different food samples

Birol, Derya; Günyar, Özlem Abaci

doi:10.1007/s00203-021-02251-4

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…It also has been isolated as a plant growth-promoting endophyte or rhizobacterium, an antagonist against plant pathogens, and a bioremediation bacterium to remedy metalliferous pollutants (Rudd et al, 1983;Shang et al, 2019;Harsha and Nair, 2020). Except K. pneumoniae reported as an EHB of Rhizopus oryzae (Birol and Gunyar, 2021), none species in the genus has been reported as an EHB. Bradyrhizobium sp.…”

Section: Discussionmentioning

confidence: 99%

Plant Growth-Promoting Ability of Mycorrhizal Fusarium Strain KB-3 Enhanced by Its IAA Producing Endohyphal Bacterium, Klebsiella aerogenes

et al. 2022

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Fusarium oxysporum KB-3 had been reported as a mycorrhizal fungus of Bletilla striata, which can promote the seed germination and vegetative growth. Endohyphal bacteria were demonstrated in the hyphae of the KB-3 by 16S rDNA PCR amplification and SYTO-9 fluorescent nucleic acid staining. A strain Klebsiella aerogenes KE-1 was isolated and identified based on the multilocus sequence analysis. The endohyphal bacterium was successfully removed from the wild strain KB-3 (KB-3−), and GFP-labeled KE-1 was also transferred to the cured strain KB-3− (KB-3+). The production of indole-3-acetic acid (IAA) in the culturing broths of strains of KE-1, KB-3, KB-3−, and KB-3+ was examined by HPLC. Their IAA productions were estimated using Salkowski colorimetric technique. The highest concentrations of IAA were 76.9 (at 48 h after inoculation), 31.4, 9.6, and 19.4 μg/ml (at 60 h after inoculation), respectively. Similarly, the three fungal cultural broths exhibited plant promoting abilities on the tomato root and stem growth. The results indicated that the ability of mycorrhizal Fusarium strain KB-3 to promote plant growth was enhanced because its endohyphal bacterium, Klebsiella aerogenes KE-1, produced a certain amount of IAA.

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Section: Discussionmentioning

confidence: 99%

Plant Growth-Promoting Ability of Mycorrhizal Fusarium Strain KB-3 Enhanced by Its IAA Producing Endohyphal Bacterium, Klebsiella aerogenes

et al. 2022

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“…and Klebsiella spp. For the genus Klebsiella, K. pneumoniae is found as endohyphal bacterium collected from Rhizopus oryzae (generated from wheat starch and bread), which is an opportunistic pathogen dangerous for human (Birol and Gunyar, 2021). In the other hand, an EHB K. aerogenes is isolated from an endophytic Fusarium oxysporum of Bletilla striata, which EHB produces IAA enhancing the growth promoting ability of the host fungi (Cheng et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Klebsiella michiganensis: A nitrogen-fixing endohyphal bacterium from Ustilago maydis

Liang

Jiang

Sun

et al. 2023

Preprint

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Ustilago maydis is a pathogenic fungus in Basidiomycota causing corn smut disease. A strain of U. maydis YZZF202006 was isolated from the tumor of corn smut collected from Jingzhou city in China. The intracellular bacteria were confirmed inner hyphal of the strain YZZF202006 by PCR amplification and fluorescence in situ hybridization (FISH) and SYTO-9. An endohyphal bacterium YZUMF202001 was isolated from the protoplasts of the strain YZZF202006. It was gram-negative, short rod-shaped with smooth light yellow colony. The endohyphal bacterium was genomic evidenced as Klebsiella michiganensis on the basis of average nucleotide identity (ANI) analysis and the phylogram. And then K. michiganensis was GFP-Labeled and reintroduced into U. maydis, which confirmed the bacterium can live in hpyphal of U.maydis. The bacterium can grow on N-free culture media. Its nitrogenase activity was reached av. 646.25 ± 38.61 nmol·mL−1·h−1 C2H4 by acetylene reduction assay. A cluster of nitrogen fixation genes (nifJHDKTXENXUSVWZMFLABQ) was found from its genome. The endohyphal K. michiganensis may play an important role to help nitrogen fixation for fungi in the future.

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“…and Klebsiella spp. For the genus Klebsiella , K. pneumoniae is found as endohyphal bacterium collected from Rhizopus oryzae (generated from wheat starch and bread), which is an opportunistic pathogen dangerous for human (Birol and Gunyar 2021 ). In the other hand, an EHB K. aerogenes is isolated from an endophytic Fusarium oxysporum of Bletilla striata , which EHB produces IAA enhancing the growth promoting ability of the host fungi (Cheng et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Klebsiella michiganensis: a nitrogen-fixing endohyphal bacterium from Ustilago maydis

Liang,

Jiang,

Sun

et al. 2023

AMB Expr

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Ustilago maydis is a pathogenic fungus in Basidiomycota causing corn smut disease. A strain of U. maydis YZZF202006 was isolated from the tumor of corn smut collected from Jingzhou city in China. The intracellular bacteria were confirmed inner hyphal of the strain YZZF202006 by PCR amplification and fluorescence in situ hybridization (FISH) and SYTO-9. An endohyphal bacterium YZUMF202001 was isolated from the protoplasts of the strain YZZF202006. It was gram-negative, short rod-shaped with smooth light yellow colony. The endohyphal bacterium was genomic evidenced as Klebsiella michiganensis on the basis of average nucleotide identity (ANI) analysis and the phylogram. Then K. michiganensis was GFP-Labeled and reintroduced into U. maydis, which confirmed the bacterium can live in hyphae of U.maydis. The bacterium can grow on N-free culture media. Its nitrogenase activity was reached av. 646.25 ± 38.61 nmol·mL− 1·h− 1 C2H4 by acetylene reduction assay. A cluster of nitrogen fixation genes (nifJHDKTXENXUSVWZMFLABQ) was found from its genome. The endohyphal K. michiganensis may play an important role to help nitrogen fixation for fungi in the future.

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Investigation of presence of endofungal bacteria in Rhizopus spp. ısolated from the different food samples

Cited by 8 publications

References 30 publications

Plant Growth-Promoting Ability of Mycorrhizal Fusarium Strain KB-3 Enhanced by Its IAA Producing Endohyphal Bacterium, Klebsiella aerogenes

Plant Growth-Promoting Ability of Mycorrhizal Fusarium Strain KB-3 Enhanced by Its IAA Producing Endohyphal Bacterium, Klebsiella aerogenes

Klebsiella michiganensis: A nitrogen-fixing endohyphal bacterium from Ustilago maydis

Klebsiella michiganensis: a nitrogen-fixing endohyphal bacterium from Ustilago maydis

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