Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Wang, Hui; Vijayavenkataraman, Sanjairaj; Wu, Yang; Zhang, Shu; Fuh, J.Y.H.

doi:10.18063/ijb.2016.01.005

Cited by 27 publications

(13 citation statements)

References 34 publications

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“…Fibre characteristics such as fibre diameter and its uniformity, were adjusted by varying several controllable process parameters [22,23] . In this study, six parameters were considered to have major effects on fibre diameter as well as morphology, and they are discussed below:…”

Section: Process Parametersmentioning

confidence: 99%

Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds

Liu¹,

Vijayavenkataraman²,

Wang³

et al. 2017

ijb

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Abstract:One of the important constituents in tissue engineering is scaffold, which provides structural support and suitable microenvironment for the cell attachment, growth and proliferation. To fabricate micro/nano structures for soft tissue repair and three-dimensional (3D) cell culture, the key is to improve fibre-based scaffold fabrication. Electrohydrodynamic (EHD) jetting is capable of producing and orientating submicron fibres for 3D scaffold fabrication. In this work, an EHD-jetting system was developed to explore the relationship between vital processing parameters and fibre characteristics. In this study, polycaprolactone (PCL) solution prepared by dissolving PCL pellets in acetic acid was used to fabricate the scaffolds. The influence of voltage, motorized stage speed, solution feed rate, and solution concentration on fibre characteristics and scaffold pattern were studied. Morphology of the EHD-jetted PCL fibres and scaffolds were analysed using optical microscope images and scanning electron microscope (SEM) images. Multi-layer scaffolds with the varied coiled pattern were fabricated and analysed. Cell attachment and proliferation have to be investigated in the future by further cell culture studies on these multi-layer coiled scaffolds.

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Section: Process Parametersmentioning

confidence: 99%

Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds

Liu¹,

Vijayavenkataraman²,

Wang³

et al. 2017

ijb

Self Cite

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“…3D bio-printing processes provide spatial control and repeatability of material deposition for attaining the design specific tissue scaffold in 3D. Three common bioprinting strategies [ 4 ] such as inkjet bioprinting [ 30 , 31 ], electro-hydrodynamic jetting [ 32 , 33 , 34 , 35 ], extrusion-based bioprinting [ 36 , 37 ] and laser-assisted bioprinting [ 38 , 39 , 40 ] are used to fabricate the 3D tissue scaffolds. Among them, the extrusion-based system is compatible with a diverse range of materials printing including hydrogels, biocompatible copolymers and their composition including heterogeneous bio-ink and cell spheroid [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

3D Printability of Alginate-Carboxymethyl Cellulose Hydrogel

et al. 2018

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Three-dimensional (3D) bio-printing is a revolutionary technology to reproduce a 3D functional living tissue scaffold in-vitro through controlled layer-by-layer deposition of biomaterials along with high precision positioning of cells. Due to its bio-compatibility, natural hydrogels are commonly considered as the scaffold material. However, the mechanical integrity of a hydrogel material, especially in 3D scaffold architecture, is an issue. In this research, a novel hybrid hydrogel, that is, sodium alginate with carboxymethyl cellulose (CMC) is developed and systematic quantitative characterization tests are conducted to validate its printability, shape fidelity and cell viability. The outcome of the rheological and mechanical test, filament collapse and fusion test demonstrate the favorable shape fidelity. Three-dimensional scaffold structures are fabricated with the pancreatic cancer cell, BxPC3 and the 86% cell viability is recorded after 23 days. This hybrid hydrogel can be a potential biomaterial in 3D bioprinting process and the outlined characterization techniques open an avenue directing reproducible printability and shape fidelity.

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“…Not all materials are easily printable into 3D porous scaffolds for experimental validation. While PCL is a widely-used biomaterial for fabrication of 3D scaffolds [ 31 , 32 ] and can be easily printed into 3D scaffolds using various 3D printing methods, optimization of parameters (nozzle diameter, flow rate, solution concentration, cross-linking) are required for printing of hydrogels made of gelatin and chitosan to fabricate 3D scaffolds of desired pore size and fiber diameter. The limitations of the fabricating method (solution viscosity, resolution, accuracy) should also be taken into account.…”

Section: Challengesmentioning

confidence: 99%

Design of Three-Dimensional Scaffolds with Tunable Matrix Stiffness for Directing Stem Cell Lineage Specification: An In Silico Study

et al. 2017

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Tissue engineering is a multi-disciplinary area of research bringing together the fields of engineering and life sciences with the aim of fabricating tissue constructs aiding in the regeneration of damaged tissues and organs. Scaffolds play a key role in tissue engineering, acting as the templates for tissue regeneration and guiding the growth of new tissue. The use of stem cells in tissue engineering and regenerative medicine becomes indispensable, especially for applications involving successful long-term restoration of continuously self-renewing tissues, such as skin. The differentiation of stem cells is controlled by a number of cues, of which the nature of the substrate and its innate stiffness plays a vital role in stem cell fate determination. By tuning the substrate stiffness, the differentiation of stem cells can be directed to the desired lineage. Many studies on the effect of substrate stiffness on stem cell differentiation has been reported, but most of those studies are conducted with two-dimensional (2D) substrates. However, the native in vivo tissue microenvironment is three-dimensional (3D) and life science researchers are moving towards 3D cell cultures. Porous 3D scaffolds are widely used by the researchers for 3D cell culture and the properties of such scaffolds affects the cell attachment, proliferation, and differentiation. To this end, the design of porous scaffolds directly influences the stem cell fate determination. There exists a need to have 3D scaffolds with tunable stiffness for directing the differentiation of stem cells into the desired lineage. Given the limited number of biomaterials with all the desired properties, the design of the scaffolds themselves could be used to tune the matrix stiffness. This paper is an in silico study, investigating the effect of various scaffold parameter, namely fiber width, porosity, number of unit cells per layer, number of layers, and material selection, on the matrix stiffness, thereby offering a guideline for design of porous tissue engineering scaffolds with tunable matrix stiffness for directing stem cell lineage specification.

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Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Cited by 27 publications

References 34 publications

Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds

Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds

3D Printability of Alginate-Carboxymethyl Cellulose Hydrogel

Design of Three-Dimensional Scaffolds with Tunable Matrix Stiffness for Directing Stem Cell Lineage Specification: An In Silico Study

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