Investigation of propofol renal elimination by HPLC using supported liquid membrane procedure for sample preparation

Abstract

One of the least explored subjects in the research on the metabolism of a widely used anaesthetic, propofol, is its excretion in an unchanged form. According to literature, the estimated percentage of applied propofol eliminated intact via kidneys is lower than 0.3%. The present study shows the amount of propofol excreted in an unchanged form with urine collected during the first 48 h after anaesthesia in five patients undergoing elective intracranial procedures. The drug was concentrated and selectively isolated from urine samples by supported liquid membrane technique and determined by HPLC with fluorescence detection. The amount of unchanged propofol eliminated with urine was approximately (0.004 +/- 0.002)% of the total applied dose. The obtained results may suggest that propofol in an unchanged form is not excreted by kidneys at all provided that all propofol determined in presented study originated from conjugates hydrolysis.

Free and bound propofol concentrations in human cerebrospinal fluid

“…Propofol was assayed by high‐performance liquid chromatography (HPLC) with fluorescence detection, according to previously published methods [13‐16]. Briefly, to each sample of plasma (1 ml), CSF (400 µl), CSF ultrafiltrate (400 µl) or plasma ultrafiltrate (400 µl), thymol, dihydrogen sodium phosphate (1 ml of 0.1 m NaH 2 PO 4 ), and cyclohexane (5 ml for plasma and 3 ml for CSF and both ultrafiltrates) were added.…”

A call for immediate climate action in anesthesiology: routine use of minimal or metabolic fresh gas flow reduces our ecological footprint

“…[70][71][72][73] Propofol is excreted almost entirely in its metabolized, inactive form. 74,75 Thus, it can be assumed that the detected propofol metabolites are primarily caused by improper disposal. Therefore, it is important to implement efficient waste disposal systems before converting to TIVA.…”

Fokus – NarCO2se und Umwelt

“…Nur geringe Mengen des Propofols werden unmetabolisiert ausgeschieden [67]. Das Umweltrisiko durch die Metabolite ist gering [67,70]. Toxikologisch scheint es zumindest wahrscheinlich, dass der Phenolring des Propofols beim Abbau einen Einfluss auf die Umwelt hat.…”