Investigation of proteins’ interaction network and the expression pattern of genes involved in the ABA biogenesis and antioxidant system under methanol spray in drought-stressed rapeseed

Golfazani, Mohammad Mohsenzadeh; Taghvaei, Mohammad Mahdi; Lahiji, Habibollah Samizadeh; Ashery, Seddigheh; Raza, Ali

doi:10.1007/s13205-022-03290-4

Cited by 9 publications

(3 citation statements)

References 70 publications

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“…Drought is one of the most serious abiotic stresses, can cause a series of injury symptoms in plants and decline the global crop yields [1,2]. In order to adapt to drought stress, plants have improved their physiological and biochemical metabolism, forming a complex and effective signal network, including ABA biosynthesis pathway [3].…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Profile of NCED Genes in Arachis hypogaea L. during Drought Stress

Chen,

Li,

Wang

et al. 2024

IJMS

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Peanut (Arachis hypogaea L.) is an important crop that provides essential proteins and oils for human and animal consumption. 9-cis-epoxycarotenoid dioxygenase (NCED) have been found can play a vital role in abscisic acid (ABA) biosynthesis and may be a response to drought stress. Until now, in Arachis hypogaea, no information about the NCED gene family has been reported and the importance of NCED-related drought tolerance is unclear. In this study, eight NCED genes in Arachis hypogaea, referred to as AhNCEDs, are distributed across eight chromosomes, with duplication events in AhNCED1 and AhNCED2, AhNCED3 and AhNCED4, and AhNCED6 and AhNCED7. Comparative analysis revealed that NCED genes are highly conserved among plant species, including Pisum sativum, Phaseolus vulgaris, Glycine max, Arabidopsis thaliana, Gossypium hirsutum, and Oryza sativa. Further promoter analysis showed AhNCEDs have ABA-related and drought-inducible elements. The phenotyping of Arachis hypogaea cultivars NH5 and FH18 demonstrated that NH5 is drought-tolerant and FH18 is drought-sensitive. Transcriptome expression analysis revealed the differential regulation of AhNCEDs expression in both NH5 and FH18 cultivars under drought stress. Furthermore, compared to the Arachis hypogaea cultivar FH18, the NH5 exhibited a significant upregulation of AhNCED1/2 expression under drought. To sum up, this study provides an insight into the drought-related AhNCED genes, screened out the potential candidates to regulate drought tolerance and ABA biosynthesis in Arachis hypogaea.

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Section: Introductionmentioning

confidence: 99%

Identification and Expression Profile of NCED Genes in Arachis hypogaea L. during Drought Stress

Chen,

Li,

Wang

et al. 2024

IJMS

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“…Therefore, identifying the NCED gene is critical in studying ABA biosynthesis and its function in plant growth and development. First discovered in the maize ABA-deletion mutant Vp14, the NCED gene provided insight into the function of NCED in ABA production (Mohsenzadeh Golfazani et al 2022 ). Later, the NCED gene family has been identified and investigated in many plant species, such as Arabidopsis (Tan et al 2003 ), cotton (Li et al 2021 ), avocado (Chernys and Zeevaart 2000 ), cowpea (Iuchi et al 2000 ), kiwifruit (Gan et al 2020 ), and grape (Wang et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and in-silico expression analysis of CCO gene family in sunflower (Helianthus annuus) against abiotic stress

Sami,

Haider,

Shafiq

et al. 2024

Plant Mol Biol

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Carotenoid cleavage oxygenases (CCOs) enzymes play an important role in plant growth and development by producing a wide array of apocarotenoids and their derivatives. These compounds are vital for colouring flowers and fruits and synthesizing plant hormones such as abscisic acid and strigolactones. Despite their importance, the gene family responsible for CCO enzymes in sunflowers has not been identified. In this study, we identify the CCO genes of the sunflower plant to fill this knowledge gap. Phylogenetic and synteny analysis indicated that the Helianthus annnus CCO (HaCCO) genes were conserved in different plant species and they could be divided into three subgroups based on their conserved domains. Analysis using MEME tool and multiple sequence alignment identified conserved motifs in the HaCCO gene sequence. Cis-regulatory elements (CREs) analysis of the HaCCO genes indicated the presence of various responsive elements related to plant hormones, development, and responses to both biotic and abiotic stresses. This implies that these genes may respond to plant hormones, developmental cues, and drought stress, offering potential applications in the development of more resistant crops. Genes belonging to the 9-cis-epoxy carotenoid dioxygenases (NCED) subgroups predominantly exhibited chloroplast localization, whereas the genes found in other groups are primarily localized in the cytoplasm. These 21 identified HaCCOs were regulated by 60 miRNAs, indicating the crucial role of microRNAs in gene regulation in sunflowers. Gene expression analysis under drought stress revealed significant up-regulation of HaNCED16 and HaNCED19, genes that are pivotal in ABA hormone biosynthesis. During organ-specific gene expression analysis, HaCCD12 and HaCCD20 genes exhibit higher activity in leaves, indicating a potential role in leaf pigmentation. This study provides a foundation for future research on the regulation and functions of the CCO gene family in sunflower and beyond. There is potential for developing molecular markers that could be employed in breeding programs to create new sunflower lines resistant to biotic and abiotic stresses.

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“…In investigating ABA biosynthesis and its function in plant growth and development, identifying the NCED gene is a signi cant turning point. First discovered in the maize ABA-deletion mutant Vp14, the NCED gene provided insight into the function of NCED in ABA production (Mohsenzadeh Golfazani et al, 2022). The NCED gene family has been identi ed and investigated in many plant species, such as Arabidopsis (Tan et al, 2003), cotton (Li et al, 2021), avocado (Chernys & Zeevaart, 2000), cowpea (Iuchi et al, 2000), kiwifruit (Gan et al, 2020), and grape (Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and In-silico Expression Analysis of CCO Gene Family in Sunflower (Helianthus annnus)

Sami,

Haider,

Shafiq

et al. 2023

Preprint

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Carotenoid cleavage oxygenases (CCOs) enzymes play an important role in plant growth and development by producing a wide array of apocarotenoids and their derivatives. These compounds are vital for colouring flowers and fruits and synthesizing plant hormones such as abscisic acid and strigolactones. Despite their importance, the gene family responsible for CCOs in sunflowers has not been studied. In this study, we identify the CCO genes of the sunflower plant to fill this knowledge gap. Phylogenetic and synteny analysis indicated that the Helianthus annnus CCO (HaCCO) proteins are conserved in different plant species and they can be divided into three subgroups based on their conserved domains. MEME analysis and multiple sequence alignment showed that conserved motifs exist in the sequence of HaCCO genes. Cis-regulatory elements (CREs) analysis of the HaCCO genes revealed the presence of multiple plant hormones and abiotic and biotic responsive elements in their promoters, suggesting that these genes might be responsive to plant hormones, developmental and drought stress, which could be utilized to develop more resilient crops. Genes belonging to the 9-cis-epoxy carotenoid dioxygenases (NCED) subgroups predominantly exhibit chloroplast localization, whereas the genes found in other groups are primarily localized in the cytoplasm. These 21 identified HaCCOs were regulated by 60 miRNAs indicating the crucial role of microRNAs in gene regulation in sunflowers. Gene expression analysis under drought stress revealed significant up-regulation of HaNCED16 and HaNCED19 genes that provide pivotal role in ABA hormone biosynthesis. While during organ-specific gene expression analysis HaCCD12 and HaCCD20 genes exhibit higher activity in leaves, indicating a potential role in leaf pigmentation. These studies provide a foundation for future research on the regulation and functions of this gene family. It could potentially be utilized to develop molecular markers that could be used in breeding programs to develop new sunflower lines.

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Investigation of proteins’ interaction network and the expression pattern of genes involved in the ABA biogenesis and antioxidant system under methanol spray in drought-stressed rapeseed

Cited by 9 publications

References 70 publications

Identification and Expression Profile of NCED Genes in Arachis hypogaea L. during Drought Stress

Identification and Expression Profile of NCED Genes in Arachis hypogaea L. during Drought Stress

Genome-wide identification and in-silico expression analysis of CCO gene family in sunflower (Helianthus annuus) against abiotic stress

Genome-Wide Identification and In-silico Expression Analysis of CCO Gene Family in Sunflower (Helianthus annnus)

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