Establishing the absolute age of palaeoearthquakes is of great significance for the assessment of the seismicity and seismic hazards of a region. As such, several different geochronological techniques to date earthquake-related material have been developed to provide answers on the time of past earthquakes. The present study is part of a wider palaeoseismic research project conducted in the Nojima Fault Zone (NFZ), where the 1995 Mw 6.9 Kobe (Japan) earthquake was triggered, to assess the suitability of the isothermal thermoluminescence (ITL) dating technique on fine-grained quartz and medium-grained feldspar and to provide a sequence of ages for fault-rock samples separated from a drilled core that was retrieved from a depth of ~506 m. Our analysis reveals that ITL can produce consistent dating results and can be considered a reliable luminescence technique for the absolute dating of fault-gouge material. The produced ITL ages signified the existence of repeated seismic events within the NFZ that took place through the late Pleistocene period, with gouge ages spanning from 78.6 ± 4.2 to 13.4 ± 1.4 ka; however, overestimation of the produced ITL dating results may be apparent. Nonetheless, even though some degree of overestimation is considered, ITL dating results denote the oldest possible age boundary of formation (or luminescence signal resetting) of the collected fault-gouge layers.