Investigation of reactive species using various gas plasmas

Takamatsu, Takeichiro; Uehara, Kodai; Sasaki, Yota; Miyahara, Hidekazu; Matsumura, Yukihiko; Iwasawa, Atsuo; Ito, Norihiko; Azuma, Takeshi; Kohno, Masahiro; Okino, Akitoshi

doi:10.1039/c4ra05936k

Cited by 179 publications

(152 citation statements)

References 37 publications

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“…Accordingly, in the present, each reactive species generated by cold atmospheric plasma irradiation was detected using ESR spectrometry, which revealed that the yield of radicals was increased in an irradiation-time-dependent manner [25]. Nevertheless, because of the existence of medium and very short life time of radicals, it is unlikely that these radicals affected the cells directly.…”

Section: Discussionmentioning

confidence: 87%

Cold atmospheric plasma enhances osteoblast differentiation

et al. 2017

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This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration.

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Section: Discussionmentioning

confidence: 87%

Cold atmospheric plasma enhances osteoblast differentiation

et al. 2017

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“…and Takamatsu et al. described the detection of radical species in plasma‐treated liquids by using various spin traps . The concentrations of the ⋅OH and ⋅OOH radical adducts of 5‐ tert ‐butoxycarbonyl‐5‐methyl‐1‐pyrroline N ‐oxide (BMPO) and 5,5‐dimethyl‐1‐pyrroline N ‐oxide (DMPO) spin traps were measured in liquid samples by Reuter and co‐workers .…”

Section: Introductionmentioning

confidence: 99%

Non‐Thermal Plasma in Contact with Water: The Origin of Species

Gorbanev

O’Connell

Chechik

2016

Chemistry A European J

269

307

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Non‐thermal atmospheric pressure plasma has attracted considerable attention in recent years due to its potential for biomedical applications. Determining the mechanism of the formation of reactive species in liquid treated with plasma is thus of paramount importance for both fundamental and applied research. In this work, the origin of reactive species in plasma‐treated aqueous solutions was investigated by using spin‐trapping, hydrogen and oxygen isotopic labelling and electron paramagnetic resonance (EPR) spectroscopy. The species originating from molecules in the liquid phase and those introduced with the feed gas were differentiated by EPR and 1H NMR analysis of liquid samples. The effects of water vapour and oxygen admixtures in the feed gas were investigated. All the reactive species detected in the liquid samples were shown to be formed largely in the plasma gas phase. It is suggested that hydrogen peroxide (determined by UV/Vis analysis) is formed primarily in the plasma tube, whereas the radical species ⋅OOH, ⋅OH and ⋅H are proposed to originate from the region between the plasma nozzle and the liquid sample.

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“…28 The agent was dissolved in a phosphate-buffered saline (-) (PBS(-)) solution, which had a pH of 7.5, and a fixed DMPO concentration of 200 mM. 29 Plasma treatments of 200 µL solutions were conducted at a 6 mm distance between the plasma source outlet and liquid surfaces with a flow rate of 0.3 L/min for 10 s. The amount of reactive species was calibrated with spin adducts of 2,2,6,6-tetramethylpiperidine 1-oxyl, which identified the amount of OH radical. Results showed that helium plasma generated the largest amounts of OH radical at 110 µM, as shown in Fig.…”

Section: -11mentioning

confidence: 99%

Atmospheric nonequilibrium mini-plasma jet created by a 3D printer

et al. 2015

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In this study, a small-sized plasma jet source with a 3.7 mm head diameter was created via a 3D printer. The jet’s emission properties and OH radical concentrations (generated by argon, helium, and nitrogen plasmas) were investigated using optical emission spectrometry (OES) and electron spin resonance (ESR). As such, for OES, each individual gas plasma propagates emission lines that derive from gases and ambient air inserted into the measurement system. For the case of ESR, a spin adduct of the OH radical is typically observed for all gas plasma treatment scenarios with a 10 s treatment by helium plasma generating the largest amount of OH radicals at 110 μM. Therefore, it was confirmed that a plasma jet source made by a 3D printer can generate stable plasmas using each of the aforementioned three gases.

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Investigation of reactive species using various gas plasmas

Abstract: In this study, atmospheric nonequilibrium plasmas were generated with six gas species using a multi-gas plasma jet.

Cited by 179 publications

References 37 publications

Cold atmospheric plasma enhances osteoblast differentiation

Cold atmospheric plasma enhances osteoblast differentiation

Non‐Thermal Plasma in Contact with Water: The Origin of Species

Atmospheric nonequilibrium mini-plasma jet created by a 3D printer

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