Study aim: This study aimed to analyze the relationships between weekly training frequency, changes in training duration, and Maximal Aerobic Speed (MAS), Maximum Oxygen Uptake (VO2max), and Isokinetic Strength over an 8-week period (pre-season 8 weeks). Material and methods: Eighteen hearing-impaired handball players (age: 26.78±2.26 years; height: 177.76±4.40 cm; body weight: 64.94±2.73 kg, training experience 13.1±2.6 years) were positioned in defense and offense based on their playing positions and were monitored for 8 weeks. Repeated Measures ANOVA test was performed for the pre-test and post-test comparisons of defense and offense players, frequency and distribution is observed, average standard deviation, maximum, and minimum values were taken. Results: The analysis results revealed a significant large positive difference in the agonist/antagonist ratio in the right extremity (p < 0.01). Conclusion: Throughout the study, fluctuating changes in the numbers and durations of training sessions were observed to significantly increase and correlate with changes in the players' fitness status. There is a statistically significant difference between pre-test and post- test values for maximal oxygen consumption (VO2max), maximal aerobic speed (MAS), and maximal heart rate (HRmax) (p< 0.01). However, the variability in the large positive difference in the agonist/antagonist ratio in the right extremity suggests that it cannot be solely explained by the number and duration of training sessions in terms of fitness level.