Investigation of Rotavirus Genotypes Infection in Egypt

Magied, M.A.; Hussein, Mai; Amer, Maysa A.; Sabah, M.; Maaty, A.A.; Ismaal, A.; El-Dougdoug, K. A.

doi:10.21608/bjas.2018.191068

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…P [8], P [4], and P[6] genotypes P [8], P [4], and P [6] are the major P genotypes, accounting for nearly 99% of all human RV infections occurring in Egypt. P [8] was the most prevalent genotype in 2002 and between 2009 and the present studies reported that P [8] represented >50% of all P genotypes detected in many regions of Egypt during the study period [56,62,64,65,67,[69][70][71]; P [4] and P [6] were the second and third most common P genotypes identified, respectively [56,61,64,[66][67][68][69]. Other investigations reported that the P [4]…”

Section: G1-g4 Genotypessupporting

confidence: 58%

“…Each genotype was predominant over a specific time period. The first study genotyping human RV, which was conducted in Egypt in 1992, revealed that G1 and G4 were equally predominant genotypes [53]; however, other reports have revealed that the G1 genotype was mostly predominant in 2002 [56], between 2009 and 2012 [62][63][64], 2015-2017 [67,68], and 2018-2020 [69,70] G4 was the predominant strain during the 1992-1993 season [53] and in 2006 [60].…”

Section: G1-g4 Genotypesmentioning

confidence: 99%

“…In Egypt, the G9 strain was first identified during the 2000-2001 rotavirus season [60]. Subsequently, G9 strains have been identified during successive seasons across the country [56,58,[61][62][63][64][65][66][67][68][69]. G12 genotype G12 was first detected in1987 in the Philippines and was designated L26.…”

Section: Clinical Significance Of Unusual G Genotypes In Egypt G9 Gen...mentioning

confidence: 99%

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The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

Ghonaim

Hopo²,

Ghonaim

et al. 2023

Zoonoses

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Acute gastroenteritis (AGE) induced by rotavirus has been a major disease burden in Egypt since 1981 when rotavirus was first reported in humans and calves. Genome segmentation facilitates the emergence of new virus serotypes, which allows reassortment during mixed infections, and is a characteristic feature of the family Reoviridae to which rotavirus belongs. The rotavirus genome involves 11 double-stranded RNA gene segments encoding 6 non-structural (NSP1–6) and 6 structural (VP1–4, VP6, VP7) proteins. Rotavirus A has a zoonotic potential associated with diarrhea. The primary strategy for prevention and control of bovine and human rotavirus infections is vaccination; however, routine rotavirus vaccination has not been implemented in the National Immunization Program. We evaluated studies published over the last 30 years that pertained to the epidemiology of circulating rotavirus genotypes among children, calves, and environmental samples in Egypt. The analysis revealed a rotavirus prevalence of 15%–100%, with diarrhea occurring throughout the year, but generally peaking during the cold months. G1 was the predominant genotype in children, followed by G2, G3, G4, G8, G9, and G12 throughout the study duration. Mixed infections were also detected. G6 was the predominant genotype in calves, followed by G10. There are still gaps in knowledge regarding molecular data of rotavirus infections in humans, animals, and environmental samples in Egypt, as well as the zoonotic potential of rotavirus disease. Therefore, it is critical to continue rotavirus surveillance in Egypt to further understand the epidemiology of rotavirus infections and the emerging new genotypes.

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Section: G1-g4 Genotypessupporting

confidence: 58%

Section: G1-g4 Genotypesmentioning

confidence: 99%

Section: Clinical Significance Of Unusual G Genotypes In Egypt G9 Gen...mentioning

confidence: 99%

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The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

Ghonaim

Hopo²,

Ghonaim

et al. 2023

Zoonoses

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Study of rotavirus genotypes G and P in one Egyptian center-cross-sectional study

Mahmoud,

Zaki,

Mohamed

et al. 2024

Ital J Pediatr

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Background Rotavirus-associated gastroenteritis is a common health problem in children, different variations of rotavirus genotypes differ according to geographic locations and the practice of wide-scale vaccination. Therefore, the present study aimed to detect both the G and P genotypes of rotavirus in children ≤ 5 years old in one center in Egypt as a cross-sectional study, to correlate the genotypes with various demographic and clinical data in infected children and to evaluate the common mixed genotypes G and P in infected children. Method The cross-sectional study included children with acute gastroenteritis ≤ 5 years old from January 2023 till March 2024 recruited from Mansoura University Children’s Hospital, Egypt based upon laboratory diagnosis by exclusion of bacterial and protozoa pathogens. The stool samples were obtained from each child and subjected to detection of rotavirus antigen by enzyme-linked immunosorbent assay (ELISA) followed by genotypes identification of G and P genotypes by nested polymerase chain reaction (PCR). Result A nested PCR study for rotavirus genotypes revealed that G1 was the most common genotype (24.7%) followed by G2 (21.1%), G3 (20%), G9 (20%), and G4 (14.1%). The genotyping of the P genotype revealed that P9 was the commonest genotype (24.7%), followed by P4 (21.2%), P10 (20%), P8 (17.6%) and P6 (16.5%). The commonest combined genotypes of G and P were G1P4 (85.7%), G3P8(88.2%), followed by G2P6 (77.8%) and G9P9(76.5%) and G4P9 (66.7%) followed by G4P10 (33.3%), G9P10(23.5%), G2P10(22.2%), G1P10 (14.3%), G3P10(11.8%). The distribution was significant (P = 0.001). The positive rotavirus antigen was more frequently detected in females (55.3%) than males (44.7%, Odd ratio 0.2, 95% CI 0.22–0.71, P = 0.001). There was a significant association between the summer season and positive rotavirus antigen (P = 0.001) and rural residence of the patients (Odd ratio 6,9 95%CI 3,5-13.5, P = 0.001). The significant associated clinical sign with positive rotavirus antigen was fever (Odd ratio 3,3, 95%CI 1,8-6.05, P = 0.001). The genotypes G and P were significantly associated with positive rotavirus antigen as all cases positive by antigen had been detected by nested PCR with the commonest genotypes G4 (24.7%, P = 0.001) and genotype P9 (24.7%, P = 0.001). Conclusion The present study highlights the common genotypes of rotavirus at one center in Egypt, G1, G2, and G3 were the commonest G genotypes. As regard genotype P the commonest genotypes were P9, P4, and P10. The commonest combined genotypes were G1P4, G3P8, G2P6. There was no effect of the practice of rotavirus vaccination at limited rates at private health sections as the rotavirus is still a major pathogen of acute gastroenteritis in children. There is a need for the inclusion of rotavirus vaccination in the national program of children vaccination in Egypt.

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Investigation of Rotavirus Genotypes Infection in Egypt

Cited by 2 publications

References 12 publications

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

The Epidemiology of Circulating Rotavirus Associated with Diarrhea in Egyptian Kids and Calves: A Review

Study of rotavirus genotypes G and P in one Egyptian center-cross-sectional study

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