Investigation of salt properties with electro-acoustic measurements and their effect on dynamic binding capacity in hydrophobic interaction chromatography

Müller, Egbert; Faude, Alexander

doi:10.1016/j.chroma.2007.11.008

Cited by 10 publications

(2 citation statements)

References 26 publications

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“…Particles for HIC were proven more stable than IEX or SEC resins. Mueller and Faude measured pH maxima in the electro‐acoustic signals of common buffers used for HIC . At low ionic strength, these maxima are at low pH, but increase with increasing buffer strength.…”

Section: Experimental Techniquesmentioning

confidence: 99%

Methods for characterization of biochromatography media

Hahn

2012

J of Separation Science

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Chromatographic methods represent the most powerful techniques for purification of biopharmaceutical compounds. Quite often, the question arises which chromatographic medium should be chosen for a particular purification task or which technique should be applied to obtain the required information for a process, respectively. The present review aims to guide through these questions by presenting experimental and modeling techniques that allow a detailed characterization and comparison of chromatography media as well provide a guideline of techniques for process development. The first section provides basic information on chromatographic theory, types of chromatographic media, and different types of techniques. The second section governs description of experimental techniques including some advises for laboratory practice. The third section presents and discusses selected references from literature. Within this article, the main focus is on traditional laboratory techniques but also automated high-throughput screening methods will briefly be discussed.

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Section: Experimental Techniquesmentioning

confidence: 99%

Methods for characterization of biochromatography media

Hahn

2012

J of Separation Science

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“…Salt ion type and salt ion concentration appear to have a strong effect on the conventional hydrophobic based proteinligand interactions. 4,5,[16][17][18] It has been shown that the effects of different ions on protein-ligand binding correlate with the Hofmeister series. 4,16,19 Hofmeister series refers to the different ability of different ions to denature protein.…”

Section: Introductionmentioning

confidence: 99%