Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods

Gülaydın, Özgül; Öztürk, Cihat; Ekin, İsmail Hakkı; Ilhan, Ziya; İlhan, Fatma

doi:10.2754/avb202392010069

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…Molecular studies have been carried out to investigate the prevalence of Toxoplasma gondii in aborted fetuses in Türkiye (Özkaraca et al 2016;Irehan et al 2022;Orunç Kılınç et al 2023;Akpinar et al 2023) In the present study, which focused on brain samples from aborted ovine fetuses, the prevalence of T. gondii infection was 16.6% (10 out of 60) based on the conventional PCR method. The clinical manifestation of toxoplasmosis in pregnant ewes can be influenced by the age and immune status of the fetuses.…”

Section: Discussionmentioning

confidence: 59%

Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

Koca,

Orunç Kılınç,

Ayan

et al. 2023

Turkish JAF Sci.Tech.

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Toxoplasma gondii, an obligatory intracellular protozoan parasite, can infect a wide range of warm-blooded animals, including livestock species. T. gondii is a zoonotic protozoan parasite that affects both humans and other warm-blooded animals. The aim of this study was to detect T. gondii by using PCR in the brain tissues of 60 aborted sheep fetuses from the Iğdır Province in Türkiye. For this purpose, 60 brain tissue samples of sheep were collected within the lambing seasons of 2023 in Iğdır, Türkiye. The DNA extraction was performed using the PureLink™ Genomic DNA Mini Kit from brain samples. The PCR was performed with the appropriate primers from the obtained DNA samples. T. gondii was found in the brain (16.6%) samples of aborted sheep fetuses. According to the present study, T. gondii infection can be one of the causes of fetus abortion of sheep in Iğdır province, Türkiye. This result emphasizes the need for vigilance and preventive measures in managing this potential public and animal health concerns.

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Section: Discussionmentioning

confidence: 59%

Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

Koca,

Orunç Kılınç,

Ayan

et al. 2023

Turkish JAF Sci.Tech.

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“…Although Chlamydia spp. strains cause abortion in small ruminants (Gülaydın et al 2023), it is reported to be determined from the eye swab samples taken from healthy animals and/or those with conjunctivitis symptoms. Thus, Polkinghorne et al (2009) examined the samples taken from a total of 128 healthy sheep and sheep with conjunctivitis symptoms for the presence of Chlamydia spp.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

Gulaydin,

Akgul

et al. 2024

Polish Journal of Veterinary Sciences

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Infectious keratoconjunctivitis is an infectious disease that negatively affects animal welfare causing systemic or local clinical signs in small ruminants and causes significant economic losses in herds. It is important to determine the etiologic agent causing the infection in the development of the protection and control strategies against the disease. The aim of this study was to determine the presence of infectious keratoconjunctivitis cases in small ruminants raised in Siirt province in Türkiye. Infectious keratoconjunctivitis was graded according to the symptoms determined by clinical examination. The presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis was investigated by PCR in swab samples obtained from the animals with keratoconjunctivitis. Infectious keratoconjunctivitis was detected in 263 (19.86%) of 1324 animals examined in the study. Of the animals with infectious keratoconjunctivitis, 163 (61.97%) were sheep and 100 (38.02%) were goats. The detection rate of infectious keratoconjunctivitis was higher in sheep than goats. In 56 (21.29%), 109 (41.44%), 67 (25.47%), and 31 (11.78%) of the cases, findings of stage 1, 2, 3, and 4 infectious keratoconjunctivitis were detected, respectively. Of the eye swab samples taken from 263 animals with infectious keratoconjunctivitis, 5 (1.90%) were positive for Mycoplasma spp. and 6 (2.28%) were positive for M. ovis. It was determined that the distribution of the bacterial agents varied according to the stage of infectious keratoconjunctivitis. No statistically significant correlation was found in the distribution of bacterial agents among identified samples according to species, sex, age, and infectious keratoconjunctivitis stage of the animals. It was thought that the data obtained in the study would contribute to the studies for protection and control by determining the incidence and aetiology of infectious keratoconjunctivitis cases observed in small ruminants.

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A six-year epidemiological study of selected zoonotic abortifacient agents in ovine and caprine foetuses in Türkiye

Şevik

2024

Epidemiol. Infect.

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Abortion is one of the major threats to the livestock industry, and it also poses significant threats to public health since some of the abortifacient agents are considered zoonotic. Chlamydia abortus (C. abortus), Coxiella burnetii (C. burnetii), Listeria monocytogenes (L. monocytogenes), and Cache Valley virus (CVV) are recognized as important zoonotic and abortifacient agents of reproductive failure in small ruminants. This study determined the prevalence of these agents in ovine and caprine foetuses in Türkiye. A total of 1 226 foetuses were collected from the sheep (n = 1 144) and goats (n = 82) from different flocks between 2012 and 2017. Molecular detection methods were used to detect C. abortus, C. burnetii, and L. monocytogenes DNA and CVV RNA in aborted foetuses. In this study, C. abortus was the most prevalent abortifacient agent among the investigated ovine (264/1144) and caprine (12/82) foetuses, followed by C. burnetii with a frequency of 2.8% (32/1144) and 8.5% (7/82) in ovine and caprine foetuses, respectively. L. monocytogenes DNA was detected in 28 (2.4%) and 2 (2.4%) of the ovine and caprine foetuses, respectively. However, CVV RNA was not detected. Although the predominant mixed infection was C. abortus and C. burnetii, mixed infection of C. abortus and L. monocytogenes, and C. burnetii and L. monocytogenes were also found. The information presented in this study contributes to the understanding of the roles of C. abortus, C. burnetii, L. monocytogenes, and CVV in abortions in small ruminants, and could be beneficial for developing more effective control strategies.

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Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods

Cited by 5 publications

References 20 publications

Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

A six-year epidemiological study of selected zoonotic abortifacient agents in ovine and caprine foetuses in Türkiye

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