Investigation of selenium pretreatment in the attenuation of lung injury in rats induced by fine particulate matters

Abstract

Selenium (Se) is vital for health because of its antioxidative and anti-inflammation functions. The aim of this study was to determine if dietary selenium could inhibit the rat lung injury induced by ambient fine particulate matter (PM). Sprague-Dawley rats were randomly allocated in seven groups (n = 8). The rats in PM exposure group were intratracheally instilled with 40 mg/kg of body weight (b.w.) of PM suspension. The rats in Se prevention groups were pretreated with 17.5, 35, or 70 μg/kg b.w. of Se for 4 weeks, respectively. Then, the rats were exposed to 40 mg/kg b.w. of PM in the fifth week. The bronchoalveolar lavage fluid (BALF) was collected to count the neutrophil numbers and to analyze the cytokines (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), soluble intercellular adhesion molecule-1 (sICAM-1)) related to inflammation, the markers related to oxidative stress (total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA)), and the indicators related to cell damage (lactate dehydrogenase (LDH), total protein (TP), alkaline phosphatase (AKP)). The lung lobe that has not undergone bronchoalveolar lavage was processed for light microscopic examination. The results showed that the proportions of neutrophils in the BALF and the pathologic scores of the lung in PM-exposed groups were higher than that in the control group (P < 0.05). Se pretreatment caused a dose-dependent decrease in TNF-α, IL-1β, sICAM-1, LDH, TP, AKP, and MDA when compared with the PM-only exposure group. Meanwhile, the dose-dependent increase in T-AOC, T-SOD, and GSH-Px activities were observed in rats pretreated with Se. In conclusion, Se pretreatment may protect rat lungs against inflammation and oxidative stress induced by PM, which suggests that Se plays an important role as a kind of potential preventative agent to inhibit the PM-induced lung injury.

A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review

“…Selenium (Se) is an essential trace element [ 1 , 2 ] that evinces antioxidant activity [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], anti-inflammatory [ 2 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ], antimutagenic [ 26 , 27 ], anticarcinogenic [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ] or chemopreventive [ 2 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ], antiviral [ 2 ], antibacterial [ 45 , 46 , 47 , 48 ], antifungal [ 49 , 50 ] and antiparasitic effects [ 51 ,…”

Insights in particulate matter‐induced allergic airway inflammation: Focus on the epithelium

“…Especially since both asthma and PM exposure have already been associated with a significant reduction in SOD or polymorphisms in genes implicated in the GSH system have been detected . Moreover, also dietary antioxidants, such as selenium, were already associated with reduced oxidative stress and lung inflammation in a model of PM‐induced lung injury . Furthermore, selenium supplementation improved symptoms in asthmatics compared to placebo …”

“…In vivo research in mice, that is using specific knockout strains and monoclonal antibodies, can provide additional insights into potential mechanisms how air pollution can modulate (allergic) airway inflammation and offer the opportunity to validate hypotheses that have been generated by human studies. First, during acute DEPs-induced inflammatory lung responses, an increased expression of IL-1b 58,59,64,[169][170][171][172][173][174][175][176][177] and GM-CSF 139,178 in BALF and lung tissue were observed, whereas 1 study reported IL-25, IL-33 and TSLP as undetectable. 139 Moreover, the increased IL-1b and GM-CSF levels could be linked to PM or DEPs-induced inflammation and AHR.…”

“…104,106,[211][212][213] Moreover, also dietary antioxidants, 209,214 such as selenium, were already associated with reduced oxidative stress and lung inflammation in a model of PM-induced lung injury. 177…”

Growth Performance and Immunity Response of Suckling Friesian Calves Fed on Ration Supplemented With Organic or Nano Selenium Produced by Lactic Acid Bacteria

“…The reduced diseases infection and mortality rate with organic and nano selenium supplementation could be attributed to the improvement in immunity response. Selenium (Se) is an essential trace element (Lu and Holmgren, 2009;Rayman, 2000) that evinces antioxidant activity (Abuelo et al, 2016;Dkhil et al, 2016;Lu et al, 2017;Ju et al, 2017), antiinflammatory (Aaseth et al, 2016;El-Ghazaly et al, 2017;Liu et al, 2017;Malhotra et al, 2016;Speckmann and Steinbrenner, 2014), antimutagenic (Peng et al, 2016;Schrauzer, 2008), anticarcinogenic (Ahmad et al, 2015;Hassan and Webster, 2016;Tran et al, 2010) or chemopreventive (Rao et al, 2001), antiviral (Hefnawy and Tórtora-Pérez, 2010) antibacterial (Cihalova et al, 2015;Guisbiers et al, 2016;Chudobova et al, 2014;Wang et al, 2015), antifungal (Guisbiers et al, 2017;Shakibaie et al, 2015) and antiparasitic effects (Beheshti et al, 2013;Dkhil et al, 2016;Mahmoudvand et al, 2014). Furthermore, it is an integral component of selenoproteins participating in a whole series of physiologically important processes (Pascual and Aranda, 2013).…”