Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China

Xie, Zhenyu; Hu, Chaoqun; Chen, C.; Zhang, L.-P.; Ren, Chen

doi:10.1111/j.1472-765x.2005.01688.x

Cited by 163 publications

(119 citation statements)

References 29 publications

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“…The plasmids were transformed into E. coli JM109 competent cells, and the sequence of the cloned genes in the vector pMD 18-T was confirmed by DNA sequencing (Invitrogen Biotech, Shanghai, China). Previous studies have shown the distribution of different virulence genes among V. cholerae, V. mimicus, V. alginolyticus and V. parahaemolyticus (Ghosh et al, 1997;Sechi et al, 2000;Shi et al, 1998;Shinoda and Nakagawa, 2004;Xie et al, 2005). Our results indicate that the virulence genes such as toxS, flaB and flaC are widely distributed among different vibrios, whereas zot and tcpA only exit in a few vibrios.…”

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confidence: 49%

Distribution of five vibrio virulence-related genes among Vibrio harveyi isolates

Bai

Pang

et al. 2008

J. Gen. Appl. Microbiol.

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Vibrio are widely distributed in aquatic environments (Thompson et al., 2006) and are commonly associated with marine living species and include many important pathogens for farmed animals and humans who consume contaminated seafood or polluted drinking water. Pathogenic mechanisms are only clearly understood for V. cholerae and V. parahaemolyticus, but not for the majority of other vibrios (Faruque et al., 1998;Nishibuchi and Kaper, 1995;Thompson et al., 2006). Many virulence genes have been elucidated, including the tdh gene (coding thermostable direct haemolysin) in V. parahemolyticus, ctxAB genes (coding the potent cholera toxin) in V. cholera and others (Faruque et al., 1998;Nishibuchi and Kaper, 1995).Zot (zonula occludens toxin) was first discovered in V. cholerae, and has the ability to increase the permeability of the small intestinal mucosa by affecting the structure of the intercellular tight junction, or zonula occludens (Fasano et al., 1991). The toxS gene was also first discovered in V. cholerae, and lies downstream from the toxR gene, which encodes the transcriptional activator for the cholera toxin (ctx ) operon in V. cholerae (Dirita and Mekalanos, 1991;Miller et al., 1989). toxS acts in conjunction with toxR to activate expression of the ctx operon (Miller et al., 1989). tcpA is an important virulence gene of V. cholerae, encoding the major subunit of the toxin-coregulation pilus (TCP), which exerts important roles in infection as the colonization factor (Faast et al., 1989). The importance of the flagellum in virulence has been demonstrated for several species, as either a motility organelle or an organelle that carries an adhesive component (Lee et al., 2006;Milton et al., 1996). V. cholerae contains five different structural genes of the flagellum: flgI, flgJ, flgM, flgL and flaA, and these genes encode the Pand L-ring proteins, the hook-associated proteins 1,3 and the flagellin core protein of the flagellum, respectively (Das et al., 1998).V. harveyi is a serious pathogen of marine animals . However, the virulence factors of V. harveyi are not completely understood. Previous studies have shown that the virulence factors, such as VHH haemolysin, protease, phospholipse, lipopolysaccharide and bacteriocin-like substances may contribute to the virulence of this species Zhang et al., 2001). It has been proposed that the virulence genes could be horizontally transferred among Vibrio spp. (Waldor and Mekalanos, 1996). Previously, we found that the vhh/tlh haemolysin gene and toxR gene exit in all V. harveyi strains detected (Pang et al

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mentioning

confidence: 49%

Distribution of five vibrio virulence-related genes among Vibrio harveyi isolates

Bai

Pang

et al. 2008

J. Gen. Appl. Microbiol.

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“…It inhabits broadly coastal seawater environments and causes many clinical infections and tremendous damage in fish, shellfish and crustaceans culture. In South China, V. alginolyticus is found to be the dominant pathogen in seawater and have caused huge loss in marine aquaculture industry (Huang and He 2002;Hu et al 2005;Xie et al 2005). The conventional culture-based method for detection of V. alginolyticus is time-consuming and labour-intensive, thus gradually replaced by PCR-based methods (Chen et al 2002;Di Pinto et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio alginolyticus is an important opportunistic bacterial pathogen for both human and aquatic animals (Reina Prieto and Heravs Palazon 1993;Caruso et al 1998;HervioHeath et al 2002;George et al 2005;Xie et al 2005;Gonzalez-Escalona et al 2006). Several clinical infections caused by V. alginolyticus were reported (Levine and Griffin 1993;Reina Prieto and Heravs Palazon 1993;GahrnHansen and Hornstrup 1994;Gomez et al 2003), as well as tremendous damage caused in fish, shellfish and crustaceans (Anderson et al 1988;Lightner 1988;Lee et al 1996;Vandenberghe et al 1999;Hörmansdorfer et al 2000;Huang and He 2002;George et al 2005;Gó mezLeó n et al 2005;Hu et al 2005;Jayaprakash et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood

Zhou

Hou

et al. 2007

Journal of Applied Microbiology

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Aim: Vibrio alginolyticus is an economically important micro‐organism. The main aim of the present study was to develop a real‐time polymerase chain reaction (PCR) assay for rapid, sensitive and effective quantification of V. alginolyticus in seawater and seafood. Methods and Results: Purified DNA of V. alginolyticus, artificially inoculated seawater and seafood tissue homogenates were subjected to the gyrB‐targeted real‐time PCR assay. Natural seawater and seafood samples were analysed by this real‐time PCR protocol. Specificity tests showed that positive result was obtained only with V. alginolyticus strains. The detection sensitivity was determined to be 0·4 pg of genomic DNA equivalent to 72 cells per PCR in pure culture and 100 cells in 1 ml of seawater or seafood tissue homogenates. Single cell detection is achieved after 3 h of sample enrichment. Conclusions: A sensitive and specific SYBR Green I‐based real‐time PCR assay targeting gyrB gene was successfully developed to quantify V. alginolyticus within 6 h in seawater and seafood samples. Significance and Impact of the Study: No report on the molecular‐based method was available for quantitative detection of V. alginolyticus. This work will provide a novel method for evaluation of the risk of V. alginolyticus to marine environmental health and seafood safety.

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“…Previous work showed that Vibrio species represents an important recipient of some V. cholerae and V. parahaemolyticus virulence genes transfers (Xie et al, 2005). Snoussi et al (2008), reported the diffusion of six V. cholerae virulence genes among 28 V. alginolyticus strains isolated from the Mediterranean seawater.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia

Mechri

Medhioub

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tIn the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrio extracellular product were also observed among HeLa and Vero cells. 308for investigation of the presence of three V. parahaemolyticus virulence genes (tlh, trh and tdh) and ten V. chole rae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and tcpP). The isolates were also tested for their cytotoxic activity towards two epithelial cells. Their pattern of resistance to antibiotics was also carried out.

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Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China

Cited by 163 publications

References 29 publications

Distribution of five vibrio virulence-related genes among Vibrio harveyi isolates

Distribution of five vibrio virulence-related genes among Vibrio harveyi isolates

Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia

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