Investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay

Li, Hong; Shen, David; O’Toole, Donal; Knowles, Donald P.; Gorham, J. R.; Crawford, Timothy B.

doi:10.1128/jcm.33.8.2048-2053.1995

Cited by 92 publications

(81 citation statements)

References 24 publications

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“…The OHV-2-specific PCR used was as previously described (2,11). Primers 556 and 775 (Table 1) were used for the primary amplification, and primers 556 and 555 (Table 1) were used for the secondary amplification.…”

Section: Cases and Background A Small Zoo Located In The North Centrmentioning

confidence: 99%

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Newly Recognized Herpesvirus Causing Malignant Catarrhal Fever in White-Tailed Deer ( Odocoileus virginianus )

Dyer²,

Keller

et al. 2000

J Clin Microbiol

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Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in the sera collected from the deer. PCR failed to amplify viral sequences from DNA extracted from peripheral blood leukocytes (PBL) and/or spleens of the deer with primers specific for ovine herpesvirus 2 (OHV-2) or specific for alcelaphine herpesvirus 1 (AHV-1). By using degenerate primers targeting a conserved region of a herpesviral DNA polymerase gene, a DNA fragment was amplified from the PBL or spleens of all six deer and sequenced. Alignment of the sequences demonstrated that the virus in the deer belongs to the Gammaherpesvirinae subfamily, exhibiting 82% identity to OHV-2, 71% to AHV-1, and 60% to a newly identified bovine lymphotropic herpesvirus. This virus, which causes classical MCF in white-tailed deer, is a newly recognized agent belonging to the MCF group of gammaherpesviruses. It is the third reported pathogenic MCF virus, genetically distinct but closely related to OHV-2 and AHV-1. The reservoir for the virus has not been identified.

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Section: Cases and Background A Small Zoo Located In The North Centrmentioning

confidence: 99%

“…It was named alcelaphine herpesvirus 1 (AHV-1), based on the subfamily of the principal reservoir host, the wildebeest (25). The virus that is endemic in sheep (11), though never isolated, has nonetheless been designated ovine herpesvirus 2 (OHV-2) (25), solely on the basis of its base sequence relatedness to AHV-1 (3).…”

mentioning

confidence: 99%

Newly Recognized Herpesvirus Causing Malignant Catarrhal Fever in White-Tailed Deer ( Odocoileus virginianus )

Dyer²,

Keller

et al. 2000

J Clin Microbiol

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“…16 Recently, a nested PCR test, based on a cloned OHV-2 gene sequence, has been developed to detect OHV-2 DNA in cattle, sheep and goats. 3,4,17,18 This test was used to support the diagnosis of SA-MCF in these deer. About 7 mL of venous blood from four of the eight deer was collected into evacuated tubes containing EDTA.…”

Section: Mfcmentioning

confidence: 99%

An outbreak of malignant catarrhal fever in young rusa deer (Cervus timorensis)

Tomkins

et al. 1997

Aust Veterinary J

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On the basis of clinical signs and histological findings eight 9-month-old male rusa deer (Cervus timorensis) were diagnosed with sheep associated-malignant catarrhal fever. Following a variable course involving rectal temperatures around 40.5 degrees C, depression, inappetence, diarrhoea, corneal opacity and hypopyon all animals died or were euthanased over a 5-week period. Severe multifocal vasculitis, mainly periglomerular and in the arcuate vessels were consistent histological findings which in the past have been adequate to confirm clinical diagnosis of sheep associated-malignant catarrhal fever. A nested polymerase chain reaction test has been used to detect a sheep associated-malignant catarrhal fever PRC product, 238 base-pairs in size, in DNA extracted from lymphocyte preparations. The result supported the diagnosis of sheep associated-malignant catarrhal fever in these deer.

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“…A number of clinical MCF cases in cattle have been examined by these laboratory methods (5,9). However, a comparative evaluation of these tests using a larger number of independent field cases has not yet been published.…”

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confidence: 99%

Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever

Müller-Doblies

Hauser

et al. 1998

J Clin Microbiol

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Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically diagnosed field cases, we have evaluated a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) and compared these assays in the diagnosis of SA-MCF in cattle with histopathology as a provisional “gold standard.” Samples from 44 cattle with clinical signs suggestive of SA-MCF were examined by histopathology, PCR, and CI-ELISA. In addition, samples from healthy cattle were evaluated by PCR (n = 96) and CI-ELISA (n = 75). Based on histopathology, 38 of the 44 clinical cases were classified as SA-MCF positive, 3 were classified as inconclusive, and 3 were classified as SA-MCF negative. The sensitivity of PCR was 95 to 97%, whereas the specificity ranged between 94 and 100%. The CI-ELISA showed a sensitivity of 56 to 87% and a specificity between 91 and 100%. In the field, there is good correlation between the diagnoses of SA-MCF by histopathology, PCR, and CI-ELISA. These data also confirm the close association of ovine herpesvirus 2 with SA-MCF in Switzerland.

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Investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay

Cited by 92 publications

References 24 publications

Newly Recognized Herpesvirus Causing Malignant Catarrhal Fever in White-Tailed Deer ( Odocoileus virginianus )

Newly Recognized Herpesvirus Causing Malignant Catarrhal Fever in White-Tailed Deer ( Odocoileus virginianus )

An outbreak of malignant catarrhal fever in young rusa deer (Cervus timorensis)

Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever

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