Investigation of Soluble HER2 and Transforming Growth Factor Beta-1 Serum Levels in Gestational Trophoblastic Disease

Dehaghani, Alamtaj Samsami; Rad, Neda Rahimi; Fattahi, Mohammad Javad; Khadang, Baharak; Kashef, Mohammad Amin; Sarraf, Zahra; Ghaderi, Abbas

doi:10.1007/s12253-008-9115-z

Cited by 2 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yang et al reported a prognostic value of 84% for percentage of cytorophoblasts with positive cytoplasmic immunostaining to predict the malignant progression of simple molar pregnancy ( 16 ). However, there are conflicting reports: Cameron et al reported the expression of c-erbB-2 in only one case out of 20 patients with persistent gestational trophoblastic disease ( 34 ); Dehaghani AS et al found that the mean serum c-erbB-2 does not differ significantly between GTD patients and normal pregnant controls ( 35 ). In contrast to these reports, we found an over expression of c-erbB-2 in cytotrophoblast of patients with GTN and complete mole with significant difference to simple molar pregnancy and partial mole.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemistry Study of P53 and C-erbB-2 Expression in Trophoblastic Tissue and Their Predictive Values in Diagnosing Malignant Progression of Simple Molar Pregnancy

et al. 2016

View full text Add to dashboard Cite

BackgroundFinding a tumor marker to predict the aggressive behavior of molar pregnancy in early stages has yet been a topic for studies.ObjectivesIn this survey we planned to study patients with molar pregnancy to 1) assess the p53 and c-erbB-2 expression in trophoblastic tissue, 2) to study the relationship between their expression intensity and progression of a molar pregnancy to gestational trophoblastic neoplasia, and 3) to determine a cut off value for the amount of p53 and c-erbB-2 expression which might correlate with aggressive behavior of molar pregnancy.Patients and MethodsIn a prospective cross sectional study by using a high accuracy technique EnVision Tm system for immunohistochemistry staining of molar pregnancy samples, we evaluated p53 and c-erbB-2 expression in cytotrophoblast and syncytiotrophoblast and the correlation of their expression with progression of molar pregnancy to gestational trophoblastic neoplasia (GTN). Normal prostatic tissue and Breast cancer tissue were used as positive controls.ResultsWe studied 28 patients with simple molar pregnancy (SMP) and 30 with GTN. Cytotrophobalst had significantly higher expression of p53 and c-erbB-2 and syncytiotrophoblast had greater expression of p53 in GTN group as compared to SMP group. The cut off values for percentage of p53 positive immunostained cytotrophoblast and syncytiotrophoblast were 5.5% and 2.5%. In c-erbB-2 positive membranous stained cytotrophoblast the cut off was 12.5%.ConclusionsOur data suggests that over expression of p53 and c-erbB-2 is associated with malignant progression of molar pregnancy. We encountered that high expression of p53 and c-erbB-2 in trophoblastic cells could predict gestational trophoblastic neoplasia during the early stages.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunohistochemistry Study of P53 and C-erbB-2 Expression in Trophoblastic Tissue and Their Predictive Values in Diagnosing Malignant Progression of Simple Molar Pregnancy

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Dave et al ( 21 ) found that higher TGF-β1 levels were exhibited in the serum of breast cancer patients. Furthermore, Dehaghani et al ( 22 ) revealed that the TGF-β1 serum levels were significantly higher in gestational trophoblastic disease patients when compared with those in pregnant and non-pregnant controls.…”

Section: Discussionmentioning

confidence: 99%

Effects of transforming growth factor-β1 on the proliferation and invasion of the HTR-8/SVneo cell line

Zuo

et al. 2014

Oncology Letters

View full text Add to dashboard Cite

Transforming growth factor-β1 (TGF-β1) is involved in the regulation of trophoblast cell proliferation and invasion. However, the mechanism underlying this process remains unknown, which is predominantly due to the difficulty in obtaining and maintaining primary trophoblast cells in culture over a long period of time. The HTR-8/SVneo cell line is an immortalized trophoblast cell line, which has been reported to exhibit a number of similar characteristics to those of parental trophoblast cells. Therefore, the cell line has been a useful tool for the investigation of placental function and tumor progression. In the present study, the HTR-8/SVneo cell line was used as a model to investigate the TGF-β1/SMAD signaling pathway in the proliferation and invasion of trophoblast cells. The proliferation and invasion ability of HTR-8/SVneo cells was determined using the MTT and Transwell assays, respectively. In addition, reverse transcription polymerase chain reactions were performed to detect the mRNA expression of a panel of known downstream mediators of TGF-β1, including TGF-β receptor I (TβRI), SMAD4, SMAD3, SMAD7 and tissue inhibitor of metalloproteinases-1 (TIMP-1). The results indicated that TGF-β1 promotes the proliferation and invasion of the HTR-8/SVneo cell line at passage 90. Furthermore, the expression of TβRI, SMAD3 and SMAD4 were reduced following treatment with TGF-β1, while the expression of SMAD7 was increased and the expression of TIMP-1 remained unchanged following TGF-β1 treatment. These observations indicated that the effects of TGF-β1 on the proliferation and invasion of the HTR-8/SVneo cell line at passage 90 were different from those of parental trophoblasts, which is in contrast to the results of previous studies. It was concluded that the HTR-8/SVneo cell lines, which have been grown for over 90 passages, do not accurately represent parental trophoblast cells in studies of the TGF-β/SMAD signaling pathway.

show abstract

Investigation of Soluble HER2 and Transforming Growth Factor Beta-1 Serum Levels in Gestational Trophoblastic Disease

Cited by 2 publications

References 23 publications

Immunohistochemistry Study of P53 and C-erbB-2 Expression in Trophoblastic Tissue and Their Predictive Values in Diagnosing Malignant Progression of Simple Molar Pregnancy

Immunohistochemistry Study of P53 and C-erbB-2 Expression in Trophoblastic Tissue and Their Predictive Values in Diagnosing Malignant Progression of Simple Molar Pregnancy

Effects of transforming growth factor-β1 on the proliferation and invasion of the HTR-8/SVneo cell line

Contact Info

Product

Resources

About