Oenothera biennis L., also known by names such as "evening primrose, calliopsis and sundrops," is a biennial plant species belonging to the Onagraceae family. The oil present in both its leaves and seeds is utilized in pharmacology and the food industry. O. biennis, being an important medicinal plant, has been analyzed for its total ash content, dry matter content, dualex index (Nitrogen Balance Index, chlorophyll, flavonol, anthocyanin), and biochemical components (total antioxidant activity, total phenolic and flavonoid content). Additionally, the amounts of a total of 13 macro and micronutrient elements, as well as heavy metals, have been determined. Based on the obtained results, it can be concluded that the species is rich in nutrient elements and biochemical content. By introducing this species into cultivation, it can be classified as a vegetable and provide a source of raw material for the pharmaceutical industry. Considering the examined parameters, it is believed that the cultivation and commercial production of this species are feasible.