Investigation of sperm hsa-mir-145-5p and MLH1 expressions, seminal oxidative stress and sperm DNA fragmentation in varicocele

Hekim, Neslihan; Gunes, Sezgin; Ergun, Sercan; Barhan, Elzem Nisa; Asci, Ramazan

doi:10.1007/s11033-024-09534-1

Cited by 1 publication

(1 citation statement)

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“…The expression of hsa-miR-145-5p has been directly linked to sperm quality. In idiopathic infertile men, the overexpression of hsa-miR-145-5p in spermatozoa results in decreased levels of its target, MLH1 mRNA, and is associated with increased seminal oxidative stress and sperm DNA fragmentation [ 40 ]. miRNAs are pivotal in influencing age-related changes in male fertility and may provide valuable insights into the mechanisms driving the decline in fertility as men age.…”

Section: Discussionmentioning

confidence: 99%

Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

Thapliyal,

Tomar,

Naglot

et al. 2024

ncRNA

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The high incidence of idiopathic recurrent pregnancy loss (iRPL) may stem from the limited research on male contributory factors. Many studies suggest that sperm DNA fragmentation and oxidative stress contribute to iRPL, but their roles are still debated. MicroRNAs (miRNAs) are short non-coding RNAs that regulate various biological processes by modulating gene expression. While differential expression of specific miRNAs has been observed in women suffering from recurrent miscarriages, paternal miRNAs remain unexplored. We hypothesize that analyzing sperm miRNAs can provide crucial insights into the pathophysiology of iRPL. Therefore, this study aims to identify dysregulated miRNAs in the spermatozoa of male partners of iRPL patients. Total mRNA was extracted from sperm samples of iRPL and control groups, followed by miRNA library preparation and high-output miRNA sequencing. Subsequently, raw sequence reads were processed for differential expression analysis, target prediction, and bioinformatics analysis. Twelve differentially expressed miRNAs were identified in the iRPL group, with eight miRNAs upregulated (hsa-miR-4454, hsa-miR-142-3p, hsa-miR-145-5p, hsa-miR-1290, hsa-miR-1246, hsa-miR-7977, hsa-miR-449c-5p, and hsa-miR-92b-3p) and four downregulated (hsa-miR-29c-3p, hsa-miR-30b-5p, hsa-miR-519a-2-5p, and hsa-miR-520b-5p). Functional enrichment analysis revealed that gene targets of the upregulated miRNAs are involved in various biological processes closely associated with sperm quality and embryonic development.

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Section: Discussionmentioning

confidence: 99%