Investigation of synergistic antimicrobial effects of the drug combinations of meropenem and 1,2-benzisoselenazol-3(2H)-one derivatives on carbapenem-resistant Enterobacteriaceae producing NDM-1

Jin, Wen Bin; Chen, Xu; Cheng, Qipeng; Qi, Xiao Lin; Gao, Wei; Zheng, Zhiwei; Chan, Edward Wai-Chi; Leung, Yun Chung; Chan, Tak Hang; Wong, Kwok Yin; Chen, Sheng; Chan, Kin‐Fai

doi:10.1016/j.ejmech.2018.06.007

Cited by 40 publications

(20 citation statements)

References 36 publications

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“…[34] The MICs of meropenem against the above-mentioned CRE strainsa re no less than 167 mm. [35] As illustrated in Ta ble2, zp3 was found to have significant inhibitory effecto navariety of clinical drug-resistantb acterial strains such as KO16, CF05, and CF35 (MIC = 8 mm). In stark contrast, the "superbug" EC33 has diminished the efficacy of meropenem by increasing the MIC of this antibiotic from less than 0.7 mm to 167 mm.…”

Section: Moderate Physicochemical Properties Endow Zp3 With Great Antmentioning

confidence: 93%

“…In stark contrast, the "superbug" EC33 has diminished the efficacy of meropenem by increasing the MIC of this antibiotic from less than 0.7 mm to 167 mm. [35] Indeed increasing drug resistance has shadowed the application of b-lactam antibiotics, offering us the impetus to research alternative approaches with AMPs.W e finally selected the best-performing peptide, namely zp3,t o evaluateits toxicity and environmental tolerance.…”

Section: Moderate Physicochemical Properties Endow Zp3 With Great Antmentioning

confidence: 99%

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Phenol‐Soluble‐Modulin‐Inspired Amphipathic Peptides Have Bactericidal Activity against Multidrug‐Resistant Bacteria

et al. 2019

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Phenol‐soluble modulins (PSMs) are a large family of cytolytic peptide toxins produced by Staphylococcus aureus. Based on their amino acid sequences, we have constructed a small library of cationic isoleucine‐rich peptides for antimicrobial evaluation. Relative to the parent PSMs, peptide zp3 (GIIAGIIIKIKK‐NH2) was found to possess greatly improved physicochemical properties (soluble in water) and antibacterial activity (MIC=8 μm for E. coli, B. subtilis, and C. freundii) while maintaining low hemolytic activity (<5 % at 256 μm) and cytotoxicity (HEK293 cells IC50>80 μm). We reasoned that the selective activity of zp3 toward bacterial cells is due to its amphiphilic nature and positive net charge. Moreover, it is difficult for bacteria to develop resistance against zp3. Through microscopic studies of E. coli, we demonstrated that zp3 can penetrate the bacterial membrane, thereby causing leakage of the bacterial cytoplasm. Our findings present a promising antimicrobial peptide lead, which has great potential for further chemical modification.

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Section: Moderate Physicochemical Properties Endow Zp3 With Great Antmentioning

confidence: 93%

Section: Moderate Physicochemical Properties Endow Zp3 With Great Antmentioning

confidence: 99%

Phenol‐Soluble‐Modulin‐Inspired Amphipathic Peptides Have Bactericidal Activity against Multidrug‐Resistant Bacteria

et al. 2019

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“…Evaluation of a newly synthesized molecule, derived from the scaffold of Ebslen (an NDM-I inhibitor) as a meropenem adjuvant was assessed by Jin et al After estimating the non-toxic adjuvant dosage, the larvae were inoculated with a lethal dose of carbapenemeresistant Enterobacter cloacae and treated with a combination of meropenem and a new compound or the two alone. Results showed a significantly reduced mortality rate when larvae were treated with the drug/ adjuvant combination, demonstrating the synergistic action of the two compounds [85].…”

Section: Antimicrobial Testingmentioning

confidence: 95%

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

et al. 2019

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A greater ethical conscience, new global rules and a modified perception of ethical consciousness entail a more rigorous control on utilizations of vertebrates for in vivo studies. To cope with this new scenario, numerous alternatives to rodents have been proposed. Among these, the greater wax moth Galleria mellonella had a preponderant role, especially in the microbiological field, as demonstrated by the growing number of recent scientific publications. The reasons for its success must be sought in its peculiar characteristics such as the innate immune response mechanisms and the ability to grow at a temperature of 37°C. This review aims to describe the most relevant features of G. mellonella in microbiology, highlighting the most recent and relevant research on antibacterial strategies, novel drug tests and toxicological studies. Although solutions for some limitations are required, G. mellonella has all the necessary host features to be a consolidated in vivo model host.

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“…To determine whether peptides work synergistically with CHA, we used the broth microdilution checkerboard method to measure the efficacy of combinations of HPRP-A1 and HPRP-A2 with CHA against Gram-negative and Gram-positive bacteria and a fungus 28. AMPs HPRP-A1 (5 μL), HPRP-A2 (5 μL) and CHA (5 μL) were serially diluted, and then 90 μL of bacteria was added to the 96-well microtitre plates to produce a final inoculum of 5×10 5 CFU/mL of bacteria and 2×10 5 CFU/mL of fungus 1.…”

Section: Methodsmentioning

confidence: 99%

<p>Functional Synergy Of Antimicrobial Peptides And Chlorhexidine Acetate Against Gram-Negative/Gram-Positive Bacteria And A Fungus In Vitro And In Vivo</p>

Zhu¹,

Huang²,

Chen³

et al. 2019

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Background and purposeTo reduce the resistance and allergic reaction to chlorhexidine acetate (CHA) in the current treatment of (Bacterial vaginosis) BV and (vulvovaginal candidiasis) VVC in female vaginitis. In this study, the antimicrobial activities and mechanism of action of the synergistic effects of antimicrobial peptides (AMPs) HPRP-A1 and HPRP-A2, and CHA, against Gram-negative and Gram-positive bacteria, and one fungus Candida albicans (C. albicans) were investigated in vitro and in mouse and rat vaginitis infection models in vivo.ResultsHPRP-A1, HPRP-A2 and CHA showed significant synergistic effects on the antimicrobial activities against different Gram-negative and Gram-positive bacteria and C. albicans. The combined application of HPRP-A2 and CHA exhibited strong synergistic effects in the mouse and rat vaginitis models caused by bacteria or C. albicans.ConclusionThis study may prompt the development of new drug combinations against vaginitis infections, including mixed bacterial and fungal infections and multi-drug-resistant infections.

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Investigation of synergistic antimicrobial effects of the drug combinations of meropenem and 1,2-benzisoselenazol-3(2H)-one derivatives on carbapenem-resistant Enterobacteriaceae producing NDM-1

Cited by 40 publications

References 36 publications

Phenol‐Soluble‐Modulin‐Inspired Amphipathic Peptides Have Bactericidal Activity against Multidrug‐Resistant Bacteria

Phenol‐Soluble‐Modulin‐Inspired Amphipathic Peptides Have Bactericidal Activity against Multidrug‐Resistant Bacteria

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

<p>Functional Synergy Of Antimicrobial Peptides And Chlorhexidine Acetate Against Gram-Negative/Gram-Positive Bacteria And A Fungus In Vitro And In Vivo</p>

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