Investigation of the algicidal exudate produced by Shewanella sp. IRI-160 and its effect on dinoflagellates

Pokrzywinski, Kaytee L.; Place, Allen R.; Warner, Mark E.; Coyne, Kathryn J.

doi:10.1016/j.hal.2012.05.002

Cited by 66 publications

(90 citation statements)

References 39 publications

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“…Previous reports generally focused on how negative interactions, including competition, predation and disturbance, affected the community's structure. Over the past two decades, many types of bacteria have been reported to kill algal cells by direct attack (Mayali and Azam, 2004) or produce algicidal substances (Pokrzywinski et al, 2012). These algicidal bacteria sometimes concurrently increase in abundance following the peak of some algal blooms, suggesting that negative interactions may affect algal bloom dynamics (Mayali and Azam, 2004; Pokrzywinski et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past two decades, many types of bacteria have been reported to kill algal cells by direct attack (Mayali and Azam, 2004) or produce algicidal substances (Pokrzywinski et al, 2012). These algicidal bacteria sometimes concurrently increase in abundance following the peak of some algal blooms, suggesting that negative interactions may affect algal bloom dynamics (Mayali and Azam, 2004; Pokrzywinski et al, 2012). In our experiment, the growth curves of A. tamarense (Figure 7) showed that axenic cultures of A. tamarense grew quickly and reached a maximum density of 3.13 × 10 4 cell/mL.…”

Section: Discussionmentioning

confidence: 99%

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Characterizing the Interactions Among a Dinoflagellate, Flagellate and Bacteria in the Phycosphere of Alexandrium tamarense (Dinophyta)

Peng

Zhou

et al. 2015

Front. Mar. Sci.

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A small flagellate alga was isolated from the phycosphere of a toxic red tide dinoflagellate Alexandrium tamarense. Phylogenetic analysis and ultrastructural observations demonstrated that the small flagellate alga is a species belong to Ochrophyte Ochromonas sp. The process of ingesting bacteria by Ochromonas sp. was recorded by a time lapse capture under a light microscope. Through the use of different assemblages in the co-culture experiment, the species interactions in this phycosphere microenvironment were analyzed. We demonstrated that the growth of Ochromonas sp. was supported by bacteria. Three strains of bacteria ingested by Ochromonas sp. were isolated and identified to belong to α-, δ-and γ-Proteobacteria. The growth of A. tamarense was suppressed when co-cultured with bacteria. In contrast, Ochromonas sp. triggered the growth of A. tamarense by inhibiting the growth of algicidal bacteria. This result firstly demonstrated a positive effect of a flagellate on a dinoflagellate in the phycosphere of A. tamarense. Combined with other negative effects between dinoflagellates and bacteria or bacteria and flagellates, this study showed a series of clear interactions among dinoflagellate, bacterium, and flagellate in the dinoflagellate microenvironment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterizing the Interactions Among a Dinoflagellate, Flagellate and Bacteria in the Phycosphere of Alexandrium tamarense (Dinophyta)

Peng

Zhou

et al. 2015

Front. Mar. Sci.

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“…Panels a and b adapted from Berman-Frank et al (2004); panels c and d adapted from Berman-Frank et al (2007); panels e and f adapted from Bar-Zeev et al (2013); panels g-k adapted from Vardi et al (2012); panels l-s adapted from Kahl et al (2008). bioactive compound (IRI-160AA) by Shewanella sp. IRI-160 induces algicidal activity in a broad range of dinoflagellates but has little or no effect on chlorophytes and cryptophytes (Pokrzywinski et al 2012, Tilney et al 2014, with most dinoflagellates exhibiting PSII inhibition and varying degrees of cellular mortality and loss of membrane integrity. Subsequent work with IRI-160AA-treated dinoflagellate cells verified PCD induction via significant increases in hydrogen peroxide, intracellular ROS, caspase 3-like activity, and inversion of phosphatidylserine in cell membranes (K.L.…”

Section: Emerging Roles Of Signaling Allelopathy and Cell-cell Intementioning

confidence: 99%

The Molecular Ecophysiology of Programmed Cell Death in Marine Phytoplankton

Bidle

2015

Annu. Rev. Mar. Sci.

135

143

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Planktonic, prokaryotic, and eukaryotic photoautotrophs (phytoplankton) share a diverse and ancient evolutionary history, during which time they have played key roles in regulating marine food webs, biogeochemical cycles, and Earth's climate. Because phytoplankton represent the basis of marine ecosystems, the manner in which they die critically determines the flow and fate of photosynthetically fixed organic matter (and associated elements), ultimately constraining upper-ocean biogeochemistry. Programmed cell death (PCD) and associated pathway genes, which are triggered by a variety of nutrient stressors and are employed by parasitic viruses, play an integral role in determining the cell fate of diverse photoautotrophs in the modern ocean. Indeed, these multifaceted death pathways continue to shape the success and evolutionary trajectory of diverse phytoplankton lineages at sea. Research over the past two decades has employed physiological, biochemical, and genetic techniques to provide a novel, comprehensive, mechanistic understanding of the factors controlling this key process. Here, I discuss the current understanding of the genetics, activation, and regulation of PCD pathways in marine model systems; how PCD evolved in unicellular photoautotrophs; how it mechanistically interfaces with viral infection pathways; how stress signals are sensed and transduced into cellular responses; and how novel molecular and biochemical tools are revealing the impact of PCD genes on the fate of natural phytoplankton assemblages.

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“…On the other hand, bacterial quorum-sensing molecules (e.g., acyl homoserine lactones) have been reported to interfere with the germination and growth of Ulva zoospores, a macroalga causing biofouling of artificial constructs [25]. In addition, different taxa of bacteria, for example, Shewanella [26], Streptomyces [27], and Bacillus [28], are known to produce algicidal metabolites. These studies have manifested complex signaling interactions in phycospheres, which we expect to exploit for controlling algae and/or bacteria in the context of biotechnology.…”

Section: Introductionmentioning

confidence: 99%