Investigation of the allergenicity alterations of shrimp tropomyosin as glycated by glucose and maltotriose containing advanced glycation end products

Abstract

Tropomyosin (TM) is the major allergen in shrimp that known to be the primary trigger for shrimp induced food allergy. Our previous reports suggest that glycation could reduce the allergenicity...

Conformation-Activity Mechanism of Alcalase Hydrolysis for Reducing In Vitro Allergenicity of Instant Soy Milk Powder

“…As shown in Figure 3A,B, the specific IgE and IgG1 binding abilities of ISMPH were significantly decreased by (64.72 ± 1.76)% and (56.79 ± 3.72)% compared to ISMP (P < 0.05). It was consistent with previous reports that soybean meal, kidney bean, black gram, and peanut were hydrolyzed by alcalase with diminished levels of IgE binding, 18,34 and ovalbumin was hydrolyzed by bromelain with lower rates of IgE and IgG 36 An obvious decrease of β-hex release in RBL-2H3 cells treated with whey protein concentrate and its hydrolysate was reported by Kim et al 37 Comprehensive in vitro evaluation results further revealed the feasibility of the optimal process described above to reduce the allergenicity of ISMP. Conformational Changes in the Reduced Allergenicity of ISMPH.…”

“…Furthermore, the EC50 values of ISMP and ISMPH were (18.90 ± 1.41) ng/mL and (70.51 ± 3.15) ng/mL, respectively, and the EC50 value of ISMPH was (73.3 ± 1.19)% lower than that of the ISMP ( P < 0.05). Yang et al revealed a clear decrement in degranulation of RBL-2H3 cells immunized with glucose-glycosylated tropomyosin . An obvious decrease of β-hex release in RBL-2H3 cells treated with whey protein concentrate and its hydrolysate was reported by Kim et al Comprehensive in vitro evaluation results further revealed the feasibility of the optimal process described above to reduce the allergenicity of ISMP.…”