Investigation of the association complex formed between dapoxetine and erythrosine‐B for facile dapoxetine assay in pharmaceutical formulation using resonance Rayleigh scattering and spectrofluorimetric techniques

Abstract

Premature ejaculation is a male sexual problem that is marked by rapid ejaculation and quick attainment of orgasm. Dapoxetine belongs to the antidepressant category and modulates its action by preventing the reuptake of serotonin by neurons. Dapoxetine is marketed as an off‐label therapy for premature ejaculation. Here, two facile, sensitive, and green compatible approaches were established for dapoxetine assay. The approaches chemically rely on association complex formed between erythrosine‐B and dapoxetine in an acidic buffered medium. The quenching effect of the formed complex on the native erythrosine fluorescence at emission 553.5 nm is simply the main idea of spectrofluorimetric assay, while resonance Rayleigh scattering methodology uses augmentation of resonance Rayleigh scattering spectrum at 345 nm by the added dapoxetine. The current approaches exhibit linearity between response and dapoxetine concentration over 0.2–2.5 μg/ml and 0.3–3.0 μg/ml for spectrofluorimetric and resonance Rayleigh scattering (RRS) methods, respectively. All variables affecting methods and complex formation were studied and precisely optimized. The criteria of validation were performed by the directives provided by International Conference on Harmonization's (ICH) Guidelines and limits of detection were 0.06 and 0.05 μg/ml for spectrofluorimetric and RRS techniques, respectively. Finally, the approaches were applied with acceptable results for pharmaceutical formulation analysis.

Design of resonance Rayleigh scattering and spectrofluorimetric methods for the determination of the antihistaminic drug, hydroxyzine, based on its interaction with 2,4,5,7‐tetraiodofluorescein: Evaluation of analytical eco‐scale and greenness/whiteness algorithms

“…Neither the fluorometric methodology nor the RRS methodology has yet been used for the quantitation of HXZ. The reaction ion pair formation between certain drugs (contain basic amine center) and erythrosin B or 2,4,5,7-tetraiodofluorescein (TDF) has been widely utilized to determine a variety of active pharmaceutical ingredients such as terbinafine, rupatadine, dapoxetine naftidrofuryl, duloxetine, amlodipine, perindopril, atomoxetine, fluvoxamine, and venlafaxine by applying either spectrofluorimetric technique or RRS technique [16][17][18][19][20][21][22][23][24]. Furthermore, TDF possesses advantageous characteristics in terms of its cost-effectiveness and long-term stability.…”

An integrative analytical approach designed for feasible tranexamic acid assay using o‐phthalaldehyde as a fluorogenic probe: applications to tablets, ampoules, and urine

“…Here, we developed a new fluorimetric method that combines the advantages of simplicity, high sensitivity, and selectivity due to the inherent sensitivity of luminescence-based methods [22][23][24]. The previously developed fluorimetric methods for TRX are affected by low sensitivity or drastic derivatization conditions.…”

Resonance Rayleigh scattering approach based on association complex formation with erythrosine B for determination of venlafaxine, application to the dosage form and spiked human plasma

“…Although ion pairs have been reported over two decades, they have recently been applied in the analysis of several pharmaceuticals using RRS in addition to spectrofluorimetry. [22][23][24][25][26][27][28][29] To date, no article has been published on VLX assays using RRS technology. The presence of basic nitrogen atoms in VLX enables its reaction with the acidic xanthene dye erythrosine B (ERB) to produce a binary complex at pH 3.4.…”

“…Although ion pairs have been reported over two decades, they have recently been applied in the analysis of several pharmaceuticals using RRS in addition to spectrofluorimetry. [ 22–29 ]…”