Investigation of the Causative Agents of Meningitis By Real-Time PCR Method

Akkaya, Oya; Güvenç, Hülya İren; Güzelant, Asuman; Kaya, Meral; Yüksekkaya, Şerife; Opuş, Ayşegül; Kurtoğlu, Muhammet Güzel; Övet, Habibe

doi:10.5222/tmcd.2017.131

Cited by 2 publications

(4 citation statements)

References 23 publications

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“…In another study from our country in which 200 CSF samples were evaluated by PCR, EV was detected in 13 (6.5%) patients. The age range with the highest EV PCR positivity was between 0–5 years 14 . In our single-center retrospective study median age of patients was younger than other Turkey multicenter study.…”

Section: Discussionmentioning

confidence: 99%

Enteroviral meningitis in children: Clinical and laboratory features with and without pleocytosis

Şen,

Ozdem,

Yalcinkaya

et al. 2024

Klin Padiatr

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Background Enteroviruses (EV) are most common causes of the etiologically known aseptic meningitis in children. EV can be detected with polymerase chain reaction (PCR) in cerebrospinal fluid (CSF) samples. We aimed to evaluate the clinical and laboratory characteristics of children diagnosed with PCR-confirmed EV meningitis in CSF samples. Patients Patients aged 1 month to 17 years who underwent lumbar puncture (LP) with suspected meningitis and had CSF viral PCR and culture results between September 2012 and January 2021 at a tertiary care hospital in Turkey were included. Methods Patients with no virus detected in CSF samples by PCR was comprised PCR-negative group. The EV PCR-positive patients were divided into two groups based on CSF pleocytosis as enteroviral meningitis (EVM) with CSF pleocytosis and EVM without CSF pleocytosis, and compared in terms of clinical and laboratory features. Results 78 (38.2%) were EV PCR-positive, and 126 (61.8%) were PCR-negative. Pleocytosis was detected in 55 (70.5%) EV PCR-positive patients and 94 (74.6%) of PCR-negative patients. Convulsion was significantly frequent (p=0.017) in EV PCR-positive patients with no pleocytosis. Protein and lactate concentrations in CSF were significantly higher in EV PCR-positive patients with pleocytosis (p=0.048, p=0.001, respectively). Median hospital stay was significantly longer in PCR-negative group (p<0.001). Conclusion Diagnosing EVM with PCR prevents long-term hospitalization, unnecessary antibiotic use and healthcare-related complications.

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Section: Discussionmentioning

confidence: 99%

Enteroviral meningitis in children: Clinical and laboratory features with and without pleocytosis

Şen,

Ozdem,

Yalcinkaya

et al. 2024

Klin Padiatr

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“…Conjugated pneumococcal polysaccharide vaccine was introduced into routine vaccination in 2008 in our country (14). In the study of Akkaya et al (6), Streptococcus pneumoniae was found to be the causative agent in 7 (3.5%) patients and ranked first among bacterial meningitis agents. In the current study Streptococcus pneumoniae was isolated in 2 female (9 and 15 years old) and 1 male (11 years old) patients (2.1%).…”

Section: Discussionmentioning

confidence: 99%

“…In our study, the most common viral agents were HHV-6, while Enteroviruses were the second most common viral agent. Akkaya et al (6) reported that Enteroviruses were the first meningitis agents with a rate of 25%. In the study conducted by Sarinoglu et al (24) in 2016, HSV-1 was the most detected factor in 12 (34.2%) patients, followed by Enterovirus detected in 8 (22.8%) patients.…”

Section: Discussionmentioning

confidence: 99%

“…The most important advantage of PCR against bacterial culture is its high sensitivity, fast results and no need for live bacteria. For this reason, it has been the most frequently used method in recent years (6,7). There may be a change in the profile of the agents of meningitis with the new vaccines that have been routinely applied in recent years (8).…”

Section: Introductionmentioning

confidence: 99%

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Menejit Ön tanısıyla Başvuran Hastalarda Beyin Omirilik Sıvı Sonuçlarının Değerlendirilmesi

Özkan

Çaycı

2023

Sağlık Bilimlerinde Değer

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Aim: It was aimed to determine the microorganisms and antibiotic susceptibilities obtained from cerebrospinal fluid samples (CSF). Material and Methods: CSF samples of 138 patients who admitted with a prediagnosis of meningitis to Ondokuz Mayıs University Pediatric Emergency Department between 01/01/2021-01/01/2022 was included. CSF cultures, identification of bacteria and their antibiotic susceptibilities were evaluated by VITEK 2 (bioMeriux, France) automated identification system. If the growth was detected by blood culture system, the specimen was cultivated onto the 5% blood agar, Eosin Methylene Blue agar and chocolate agar. Also, CSF samples were studied for both viral and bacterial agents with the Smart Cycler (Cepheid, USA) device working with Real-Time PCR method. In this system, Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, Cytomegalovirus, Herpes simplex type-1, Herpes simplex type-2, Human herpes virus type-6 (HHV-6), Varicella zoster virus and Epstein Barr virus agents were detected. Results: Of the 138 CSF samples, 50,88% were male and 49,12% were female. The mean age of the patients was 36 ± 55 months. Bacterial agents were detected in 18 (13,8%) of the CSF samples and viral agents in 12 (9,2%) samples. Staphylococcus epidermidis (64.6%), Staphylococcus aureus (7.7%) and Streptococcus pneumonia (6.9%) took the first three places in bacterial agents. Among the viral agents, HHV-6 was the most common followed by Enteroviruses. Conclusion: It has been observed that the profiles and resistance status of microorganisms frequently seen in CSF have changed over the years.

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Investigation of the Causative Agents of Meningitis By Real-Time PCR Method

Cited by 2 publications

References 23 publications

Enteroviral meningitis in children: Clinical and laboratory features with and without pleocytosis

Enteroviral meningitis in children: Clinical and laboratory features with and without pleocytosis

Menejit Ön tanısıyla Başvuran Hastalarda Beyin Omirilik Sıvı Sonuçlarının Değerlendirilmesi

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