We report the precise study of orbital angular momentum transfer from Laguerre-Gaussian (LG) beams to submicrometer-sized dielectric spheres. Stable three-dimensional off-axis trapping of the spheres was achieved and confirmed by the constant behavior of the sphere's revolution radius against the trapping LG-beam power. By measuring the revolutions of the sphere that was trapped and revolved in mid-water, we confirmed excellent linearity between the revolution rate and the trapping light free from the hydrodynamic coupling with walls of a sample chamber. This result suggests a torque source of precise controllability, which will contribute to the study of biological molecules, nonequilibrium statistical physics, and micromachines.Keywords Holographic optical tweezers Á Optical vortices Á Phase only spatial light modulator Optical vortices (OVs) are known as a series of light beams with singular wavefronts. Due to their phase property, OVs carry orbital angular momenta around their beam axes to have an ability of revolving objects under light irradiation [1,2]. This suggests the possibility of maintaining the objects' revolutions by continuous irradiation of OVs without any dynamical control. Such revolutions have been achieved two-dimensionally on cover glasses [3][4][5] except for a few studies reporting three-dimensional revolution in mid-water with the help of gravity [6] or an additional Gaussian beam [7] for fixing the axial trapping positions. Whether a single OV can achieve three-dimensional trapping in cooperation with object's revolution remains unclear, but the authors [8] recently established direct evidence of three-dimensional trapping by LaguerreGaussian (LG) beams that are the most common embodiments of OVs.In this report, we study the controllability of the revolution of micrometer-sized polystyrene spheres trapped by LG beams in mid-water. The trapping LG beams were generated by a holographic phase-amplitude modulation scheme to obtain high-quality ones [9]. To suppress the interaction of the trapped spheres with walls caused by Brownian motion in the axial direction, we observed the revolution of polystyrene spheres trapped in mid-water more than 1.5 lm away from the surface of a cover glass. Measurements of revolution speed with varying LG-beam power revealed precise controllability of the revolutions by the trapping light power, suggesting a novel optical tool that is especially useful for investigating the torsional properties of biological molecules [10] and driving rotational micromachines [11]. Figure 1 schematically shows the present experimental setup. The linearly-polarized laser output (wavelength k ¼ 1; 064 nm) was expanded by a beam expander and shaped into a top-hat intensity profile through an aperture. The trapping LG beams were obtained by converting the top-hat light with holographic phase patterns displayed on a liquid-crystal-on-silicon spatial light modulator (LCOS-SLM). The holographic patterns were prepared by a simultaneous phase-amplitude modulation method to gen...