Investigation of the changes in the lipid profiles in hairtail (Trichiurus haumela) muscle during frozen storage using chemical and LC/MS-based lipidomics analysis
“…Specific lipid fingerprint profiles were generated using MS, followed by real-time lipidomic analysis of the samples. REIMS has been shown to provide real-time, high-throughput, and in situ analyses without the need for any sample pretreatment and has demonstrated excellent competence in food safety, quality, and provenance widely. − It has been reported in our laboratory that the authentication of four tuna samples was carried out using the iKnife-REIMS method based on the differences in FAs and PLs . This proves the feasibility of the REIMS method for real-time and in situ lipidomic fingerprint and species differentiation.…”
Bigeye tuna (BET, Thunnus obesus) is one of the most nutritious and luxurious cosmopolitan fish. The cooked BET products are capturing the interests of consumers by enhancing flavor and ensuring microbiological safety; however, the lipidomic fingerprints during daily cooking processes have not been investigated. In this work, lipid phenotypic data variation in BET during air-frying, roasting, and boiling was studied comprehensively using iKnife rapid evaporative ionization mass spectrometry (REIMS). The outstanding lipid ions mainly including fatty acids (FAs) and phospholipids (PLs) were identified structurally. It was demonstrated that the rates of heat transfer and lipid oxidation in air-fried BET were slower than those in roasted and boiled BET by elucidating the lipid oxidation and PL hydrolysis mechanism. Furthermore, multivariate REIMS data analysis (e.g., discriminant analysis, support vector machine, neutral network, and machine learning models) was used to characterize the lipid profile change in different cooked BET samples, among which FA C22:6 , PL 18:3/22:6 , PL 18:1/22:6 , and others were the salient contributing features for determining the cooked BET samples. These results may provide a potential strategy for a healthy diet by controlling and improving functional food quality in daily cooking.
“…Specific lipid fingerprint profiles were generated using MS, followed by real-time lipidomic analysis of the samples. REIMS has been shown to provide real-time, high-throughput, and in situ analyses without the need for any sample pretreatment and has demonstrated excellent competence in food safety, quality, and provenance widely. − It has been reported in our laboratory that the authentication of four tuna samples was carried out using the iKnife-REIMS method based on the differences in FAs and PLs . This proves the feasibility of the REIMS method for real-time and in situ lipidomic fingerprint and species differentiation.…”
Bigeye tuna (BET, Thunnus obesus) is one of the most nutritious and luxurious cosmopolitan fish. The cooked BET products are capturing the interests of consumers by enhancing flavor and ensuring microbiological safety; however, the lipidomic fingerprints during daily cooking processes have not been investigated. In this work, lipid phenotypic data variation in BET during air-frying, roasting, and boiling was studied comprehensively using iKnife rapid evaporative ionization mass spectrometry (REIMS). The outstanding lipid ions mainly including fatty acids (FAs) and phospholipids (PLs) were identified structurally. It was demonstrated that the rates of heat transfer and lipid oxidation in air-fried BET were slower than those in roasted and boiled BET by elucidating the lipid oxidation and PL hydrolysis mechanism. Furthermore, multivariate REIMS data analysis (e.g., discriminant analysis, support vector machine, neutral network, and machine learning models) was used to characterize the lipid profile change in different cooked BET samples, among which FA C22:6 , PL 18:3/22:6 , PL 18:1/22:6 , and others were the salient contributing features for determining the cooked BET samples. These results may provide a potential strategy for a healthy diet by controlling and improving functional food quality in daily cooking.
“…The significant changes in the lipid profiles were attributed to lipid oxidation in the ADH muscle, most of which were significantly reduced (low abundance) after the air-drying process. The changes in lipid profiles could be attributed to lipid decomposition, side-chain modifications during lipid oxidation, or oxygen- and salt-promoted lipid oxidation ( Tu et al, 2022 , Fang et al, 2022 ). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…TGs gradually reduced as DGs increased during the air-drying process, which was attributed to the hydrolysis of TG to generate DG ( Yuan et al, 2018 ). The accumulation of DGs might caused by the decomposition of GPs ( Fang et al, 2022 , Wang et al, 2020 ). TG is broken down into glycerol and FAs by the action of a suite of lipases, increasing the UFAs.…”
Section: Resultsmentioning
confidence: 99%
“…Compared with FH, the accumulated abundance levels of several Cers significantly increased, and the accumulated abundance level of SM significantly declined in the ADH samples, indicating that the air-drying method can promote the conversion of SMs to Cers. The loss of the N -linked fatty acyl chain and one or two water molecules may have caused the sphingoid backbone to fragment, which may be the cause of the greater abundance of Cers in the ADH samples ( Fang et al, 2022 , Granafei et al, 2017 ). However, the increased Cers abundance was also attributed to the oxidation of glycosphingolipids induced by free radicals ( Couto et al, 2015 ).…”
“…Lipidomic can conduct a comprehensive study of molecular lipids in cells, tissues, and organisms, 24 and can be used to identify lipid metabolites in experimental subjects and analyze metabolic pathways related to lipid metabolism. Fang et al 25 used LC/MC-based lipidomic to explore mass spectrometry changes in frozen hairtail muscle over 120 days, and 153 high-level differential lipids were detected compared to fresh hairtail. However, in addition to identifying lipid metabolites during metabolism, we would like to further explore which genes are affected by lipid metabolism and reveal the link between differential metabolites and differentially expressed genes (DEGs).…”
Large yellow croaker roe phospholipids were found to be able to regulate the lipid metabolism of normal-diet mice mainly through influencing the gene expression in the liver closely related to the absorption and digestion of lipids.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
