Investigation of the cutaneous penetration behavior of dexamethasone loaded to nano-sized lipid particles by EPR spectroscopy, and confocal Raman and laser scanning microscopy

Lohan, Silke B.; Saeidpour, Siavash; Solik, Agnieszka; Schanzer, Sabine; Richter, Heike; Dong, Pin; Darvin, Maxim E.; Bodmeier, Roland; Patzelt, Alexa; Zoubari, Gaith; Unbehauen, Michael; Haag, Rainer; Lademann, Jürgen; Teutloff, Christian; Bittl, Robert; Meinke, Martina C.

doi:10.1016/j.ejpb.2016.12.018

Cited by 25 publications

(27 citation statements)

References 47 publications

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“…The loading efficacy and localization of DxPCA within the amide CMS nanocarriers was determined and published previously. 23,24 A glucocorticoid-containing cream, which is typically used as an anti-inflammatory therapeutic in the oral cavity is the commercially available Dontisolon ® D. Therefore, a similar control unguent was formulated that contained DxPCA and Macrogol 400 (92% m/m), 1500 (4% m/m) and 4000 (4% m/m) (Caesar & Loretz, Hilden, Germany). This control unguent was prepared with pestle and mortar performing the following steps: production of the base by homogenization of melted macrogols until room temperature was reached, homogeneous dispersion of drug into base.…”

Section: Dexamethasone Uptake and Release From Nanocarriers Into Ormentioning

confidence: 99%

“…For the MTT assay, 1 × 10 4 cells were seeded into 96 well plates and were grown to 60-70% confluence. Subsequently, the culture medium was removed and replaced with 100 μL/well of fresh medium containing different concentrations of the CMS nanocarrier for 24,48 and 72 hours. Then, 10 μL MTT solution per well were added and the plates were incubated for 4 hours at 37°C.…”

Section: Cell Viability Assaysmentioning

confidence: 99%

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Characterization of hyperbranched core‐multishell nanocarriers as an innovative drug delivery system for the application at the oral mucosa

Jäger

Obst

Lohan

et al. 2017

J of Periodontal Research

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Background and Objectives In the oral cavity, the mucosal tissues may develop a number of different pathological conditions, such as inflammatory diseases (gingivitis, periodontitis) and autoimmune disorders (eg, oral lichen planus) that require therapy. The application of topical drugs is one common therapeutic approach. However, their efficacy is limited. Dilution effects due to saliva hinder the adherence and the penetration of drug formulations. Therefore, the bioavailability of oral topical drugs is insufficient, and patients may suffer from disease over years, if not life‐long. Material and Methods In the present study, we characterized core‐multishell (CMS) nanocarriers for their potential use as drug delivery systems at oral mucosal tissues. For this purpose, we prepared porcine masticatory as well as buccal mucosa and performed Franz cell diffusion experiments. Penetration of fluorescently labeled CMS nanocarriers into the mucosal tissue was analyzed using confocal laser scanning microscopy. Upon exposure to CMS nanocarriers, the metabolic and proliferative activity of gingival epithelial cells was determined by MTT and sulforhodamine B assays, respectively. Results Here, we could show that the carriers penetrate into both mucosal tissues, while particles penetrate deeper into the masticatory mucosa. Electron paramagnetic resonance spectroscopy revealed that the 3‐carboxy‐2,2,5,5‐tetramethyl‐1‐pyrrolidinyloxy‐labeled glucocorticoid dexamethasone loaded on to the CMS nanocarriers was released from the carriers in both mucosal tissues but with a higher efficiency in the buccal mucosa. The release from the nanocarriers is in both cases superior compared to the release from a conventional cream, which is normally used for the treatment of inflammatory conditions in the oral cavity. The CMS nanocarriers exhibited neither cytotoxic nor proliferative effects in vitro. Conclusion These findings suggested that CMS nanocarriers might be an innovative approach for topical drug delivery in the treatment of oral inflammatory diseases.

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Section: Dexamethasone Uptake and Release From Nanocarriers Into Ormentioning

confidence: 99%

Section: Cell Viability Assaysmentioning

confidence: 99%

Characterization of hyperbranched core‐multishell nanocarriers as an innovative drug delivery system for the application at the oral mucosa

Jäger

Obst

Lohan

et al. 2017

J of Periodontal Research

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“…Eklouh-Molinier [211] combined CRM and cutometric data into a Partial Least-Squares (PLS) model in an attempt to profile spectral markers associated with ageing, and used this to create a "biological age" chart that mapped skin age based on its molecular state rather than its chronological age. Besides SC thickness and water content, measurements of ceramides/fatty acids and NMF content are possible with CRM, and can be used to assess barrier function [215,216], as well as the penetration of sunscreens [217,218], cosmetic ingredients [219][220][221], and other substances [220,[222][223][224][225][226][227][228].…”

Section: Confocal Raman Microscopymentioning

confidence: 99%

Review of Modern Techniques for the Assessment of Skin Hydration

Qassem

Kyriacou

2019

Cosmetics

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Skin hydration is a complex process that influences the physical and mechanical properties of skin. Various technologies have emerged over the years to assess this parameter, with the current standard being electrical probe-based instruments. Nevertheless, their inability to provide detailed information has prompted the use of sophisticated spectroscopic and imaging methodologies, which are capable of in-depth skin analysis that includes structural and composition details. Modern imaging and spectroscopic techniques have transformed skin research in the dermatological and cosmetics disciplines, and are now commonly employed in conjunction with traditional methods for comprehensive assessment of both healthy and pathological skin. This article reviews current techniques employed in measuring skin hydration, and gives an account on their principle of operation and applications in skin-related research.

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“…The number of analytical methods for measuring penetration properties of drugs are limited. Most commonly, the skin is incubated with the formulation to be tested, and segmented afterwards, either by removing the SC step by step with adhesive tape strips, or by cutting it using a cryo-microtome [ 7 , 8 , 9 ]. The amount of drugs in each layer can be determined by High-performance liquid chromatography (HPLC) subsequently.…”

Section: Introductionmentioning

confidence: 99%

A New Method for In-Situ Skin Penetration Analysis by Confocal Raman Microscopy

Krombholz

Lunter

2020

Molecules

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In the development of dermal drug formulations and cosmetics, understanding the penetration properties of the active ingredients is crucial. Given that widespread methods, including tape stripping, lack in spatial resolution, while being time- and labour-intensive, Confocal Raman Microscopy is a promising alternative. In optimizing topically applied formulations, or the development of generic formulations, comparative in-situ measurements have a huge potential of saving time and resources. In this work, we show our approach to in-situ skin penetration analysis by confocal Raman Microscopy. To analyse feasibility of the approach, we used caffeine solutions as model vehicles and tested the effectiveness of 1,2-pentanediol as a penetration enhancer for delivery to the skin.

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Investigation of the cutaneous penetration behavior of dexamethasone loaded to nano-sized lipid particles by EPR spectroscopy, and confocal Raman and laser scanning microscopy

Cited by 25 publications

References 47 publications

Characterization of hyperbranched core‐multishell nanocarriers as an innovative drug delivery system for the application at the oral mucosa

Characterization of hyperbranched core‐multishell nanocarriers as an innovative drug delivery system for the application at the oral mucosa

Review of Modern Techniques for the Assessment of Skin Hydration

A New Method for In-Situ Skin Penetration Analysis by Confocal Raman Microscopy

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