Investigation of the Cytotoxicity of Electrospun Polysuccinimide-Based Fiber Mats

Abstract

This study investigated cell viability in the presence of allylamine-modified and plasma-treated electrospun polysuccinimide fiber mats (PSI-AAmp). Low pressure non-equilibrium plasma was used for crosslinking the PSI-AAm. Comparison of FTIR and XPS analyses demonstrated that crosslinking occurred on the surface of the samples. Cell viability was investigated using the MG-63 osteosarcoma cell line and WST-1 viability reagent. Since PSI hydrolyzes to poly(aspartic acid) (PASP), PASP was used in addition to the regular controls (cells only). Phase contrast showed normal morphology in all cases at 24 h; however, in the presence of PSI-AAmp at 72 h, some rounded, dead cells could also be seen, and proliferation was inhibited. Since proliferation in the presence of PASP alone was not inhibited, the cause of inhibition was not the final product of the hydrolysis. Further investigations will be carried out to pinpoint the cause.

Enhancing critical features of poly(amino acid) based meshes

“…According to several studies, cell adhesion, proliferation and differentiation was higher on scaffold composed of thinner fibers 24,41 . As PSI is a poly(amino acid) and a biocompatible and biodegradable polymer, 13,42 PSI fibrous systems with advantageous fiber sizes would greatly complement an already promising candidate for tissue engineering. On the other hand, a nanofibrous drug carrier system could also benefit as the total surface area of the system is dramatically increased 43 …”

“…Therefore, comprehensive studies including examinations of multiple parameters are indispensable as they can provide insights into how a specific polymer or polymer family behaves and can be exploited. 10,12 PSI is hydrolyzed in slightly alkaline media and water (albeit quite slowly in the latter) and recent studies have already demonstrated its cyto-and potential biocompatibility 13,14 therefore it could be considered as a candidate for environmental and biomedical applications. 15 The following work aimed to investigate which electrospinning parameters are crucial during the fabrication of a poly(amino acid) system, namely polysuccinimide, in regards to fiber quality and size.…”

“…PSI is hydrolyzed in slightly alkaline media and water (albeit quite slowly in the latter) and recent studies have already demonstrated its cyto‐ and potential biocompatibility 13,14 therefore it could be considered as a candidate for environmental and biomedical applications 15 …”

Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

“…In parallel research we work on the development of scaffolds that allow not only the survival and proliferation of the ectomesenchyme-derived cells, both in vitro and in vivo, but also elicit their differentiation [64][65][66][67][68]. By chemical modification of biocompatible and biodegradable poly(aspartamide) (PASP)-based hydrogels, and parallel studies on their biocompatibility, biodegradability and regenerative potential, we are attempting to develop novel scaffolds that are clinically applicable [64][65][66][67][68]. We expect that in the near future these separate lines of research, on microcarriers and scaffolds, will meet through the development of biomaterials that can serve both for cell expansion and tissue regeneration.…”

A Convenient and Universal Strategy toward Solvent‐Tolerant Microporous Structure for High‐Performance Wearable Electronics and Smart Textiles

“…[33,37,39] This makes plasma treatment a valuable strategy for achieving solvent-tolerant modification in a variety of applications. [40][41][42][43][44][45][46] However, rare studies have been conducted to assess the possibility of using plasma treatment to enhance the solvent-tolerant properties of existing micro/nanostructures on fiber and textile surfaces. Successful implementation of this approach for fabrication of solventtolerant micro/nanostructures on complex woven and fabrics, could offer a new avenue for the practical application of wearable electronics and smart textiles.…”