Investigation of the Effect of Magnetite Nanoparticles (MCS-B) on Human Platelet Aggregation

Abstract

Background: Currently, one of the main problems that happens in the treatment by extracorporeal methods of hemosorption is systemic shifts in homeostasis. Among these complications the most significant are blood cells traumatization and hemorrhage. Direct physical contact of blood with sorbent surface causes activation of a multistage reaction of thrombosis. Now, new methods of hemosorption using non-traumatic sorbents are being actively developed. Wide introduction of nanotechnological preparations (magnetite nanoparticles) in clinical medicine allows improving methods of hemocorrection, creating a new class magnetically sorbent. The aim of this study is to know the activity of magnet-controlled sorbent inhibiting the aggregation of platelets in an in vitro model. Materials and Methods: 0.9% NaCl, magnetite nanoparticles of magnet-controlled sorbent (MCS-B brand). Object of research: platelets in relatively healthy volunteers. Quantitative determination aggregation of platelets activity by using aggregometer A-1 was carried out by the Bornov's method in the modification of Zachary and Kinah. Results and discussions: The results of the study showed that the use of NaCl saline solution shifts the colloidal suspension equilibrium of platelets towards a significant (P<0.001) increase in the rate and index of their aggregation. For the first time, the effect of 0.9% NaCl on function of platelets makes us reconsider the concept of safety of infusion solutions in patients with initial signs of platelet disorders hemostasis. On the contrary, the use of MCS-B nanoparticles significantly revealed (P<0.05) an increase in the stability of colloidal suspension of platelets. This is an important pathogenetic factor which affects the occurrence of correction of hemostasis in conditions of blood clotting disorders.

Bacterial-Nanocellulose-Based Biointerfaces and Biomimetic Constructs for Blood-Contacting Medical Applications

“…Studies have shown that magnetic fields can affect blood flow through orientation of blood cells and can potentially limit thrombosis through promoting blood flow . Additionally, studies have shown that magnetic nanoparticles reduce platelet aggregation . Therefore, an important extension of this research would be testing magnetized BNC for thrombogenicity.…”

“… 163 Additionally, studies have shown that magnetic nanoparticles reduce platelet aggregation. 164 Therefore, an important extension of this research would be testing magnetized BNC for thrombogenicity.…”

A New Promising Method of Hepatitis Treatment at the Level of Ultrastructure of the Liver by Standardized Powder form of Magnetite Nanoparticles (Micromage-B)

“…The enumerated parameters for reliable have influence on haemorreology and state of microcirculation on the whole. The high local concentration of magnetite in vessels is caused by disturbances of blood circulation, microcirculation and hypoxia of tissues [10,11]. It is dangerous in main vital organs: brain, heart, lungs, liver and kidneys.…”

“…It was found in the choice of theme of the present investigation. The task was set in an experiment on animals to check possibility of the use of the before worked-out and studied methodology of using standardized form of magnetite nanoparticles [11][12][13][14][15][16][17][18][19][20]. The main purpose is to investigate the ultrastructural reconstructions in hepatic cells after using standardized powder form of magnetite nanoparticles Micromage-B [21][22][23].…”