Investigation of the effect of biologically active threo-Ds-isocitric acid on oxidative stress in <i>Paramecium caudatum</i>

Morgunov, Igor G.; Карпухина, О.В.; Kamzolova, Svetlana V.; Samoilenko, Vladimir A.; Иноземцев, А. Н.

doi:10.1080/10826068.2017.1381622

Cited by 26 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown earlier [7,8], ICA displays antioxidant properties and prevents oxidative stress caused by heavy metals. In particular, ICA counteracts the negative effect of oxidative stress on the learning and memory of animals.…”

Section: Effect Of Ica On the Spatial Memory Of Ratsmentioning

confidence: 52%

“…The interest in isocitric acid (ICA) is associated with its potential efficiency in the treatment of Parkinson's disease, Fe-deficiency anemia, and metabolic myopathies associated with a shortage of succinate dehydrogenase and aconitate hydratase, as well as the mutation of the gene of NADP-dependent isocitrate dehydrogenase 1 (IDH1) as one possible reason for the emergence of neoplasms [1][2][3][4][5][6]. In experiments with oxidative stress induced by the action of hydrogen peroxide and ecotoxic heavy metals (Cu, Pb, Zn, Cd) on the Paramecium caudatum infusoria cells, the monopotassium salt of ICA was found to be a more efficient antioxidant than ascorbic acid [7]. ICA monopotassium salt also mitigates the neurotoxic effect of lead and molybdenum salts, as is evident from its beneficial effect on the learning and memory inhibition in rats intoxicated with these heavy metal salts [8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

Self Cite

View full text Add to dashboard Cite

Within this work, the microbial synthesis of (2R,3S)-isocitric acid (ICA), a metabolite of the nonconventional yeast Yarrowia lipolytica, from biodiesel waste, has been studied. The selected strain Y. lipolytica VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions. The maximal production of ICA with the minimal formation of the byproduct was provided by the use of a concentration of (NH4)2SO4 (6 g/L); the addition of biodiesel waste to cultivation medium in 20–60 g/L portions; maintaining the pH of the cultivation medium at 6, and degree of aeration between 25% and 60% of saturation. Itaconic acid at a concentration of 15 mM favorably influenced the production of ICA by the selected strain. The optimization of cultivation conditions allowed us to increase the concentration of ICA in the culture liquid from 58.32 to 90.2 g/L, the product yield (Y) by 40%, and the ICA/CA ratio from 1.1:1 to 3:1. Research on laboratory animals indicated that ICA counteracted the negative effect of ammonium molybdate (10−5 М) and lead diacetate (10−7 М) on the learning and spatial memory of rats, including those exposed to emotional stress.

show abstract

Section: Effect Of Ica On the Spatial Memory Of Ratsmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Utilization of respiratory chains for aerobic metabolism comes along with the generation of ROS [ 6 , 9 ]. To eliminate these toxic byproducts, aerobic organisms developed antioxidant defense mechanisms including enzymatic and non-enzymatic protection systems [ 12 , 13 , 14 , 15 , 16 , 17 ]. The abundant LMW thiol MSH functions to maintain the reduced state of the cytoplasm and represents the main non-enzymatic antioxidant in high-GC Gram-positive bacteria, such as the industrial platform organism C. glutamicum [ 19 , 27 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…An excess of ROS leads to oxidative stress, which subsequently damages essential cell components, such as lipids, proteins, and nucleic acids [ 11 ]. To avoid irreversible damages, aerobic living organisms possess a wide range of ROS-scavengers including enzymatic and non-enzymatic detoxification systems [ 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations

et al. 2020

View full text Add to dashboard Cite

In aerobic environments, bacteria are exposed to reactive oxygen species (ROS). To avoid an excess of ROS, microorganisms are equipped with powerful enzymatic and non-enzymatic antioxidants. Corynebacterium glutamicum, a widely used industrial platform organism, uses mycothiol (MSH) as major low molecular weight (LMW) thiol and non-enzymatic antioxidant. In aerobic bioreactor cultivations, C. glutamicum becomes exposed to oxygen concentrations surpassing the air saturation, which are supposed to constitute a challenge for the intracellular MSH redox balance. In this study, the role of MSH was investigated at different oxygen levels (pO2) in bioreactor cultivations in C. glutamicum. Despite the presence of other highly efficient antioxidant systems, such as catalase, the MSH deficient ΔmshC mutant was impaired in growth in bioreactor experiments performed at pO2 values of 30%. At a pO2 level of 20%, this growth defect was abolished, indicating a high susceptibility of the MSH-deficient mutant towards elevated oxygen concentrations. Bioreactor experiments with C. glutamicum expressing the Mrx1-roGFP2 redox biosensor revealed a strong oxidative shift in the MSH redox potential (EMSH) at pO2 values above 20%. This indicates that the LMW thiol MSH is an essential antioxidant to maintain the robustness and industrial performance of C. glutamicum during aerobic fermentation processes.

show abstract

“…There is an ever-growing interest for natural bioactive molecules which can be used in different industrial sectors. Other than microalgae [10,11], other microorganisms such as yeasts were found to be able to produce biologically active compounds useful for their antioxidant activity [15,16].…”

Section: Introductionmentioning

confidence: 99%

Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass

2019

View full text Add to dashboard Cite

Arthrospira platensis (spirulina) is considered a source of natural molecules with nutritional and health benefits. As the different storage forms can affect the quantity and quality of bioactive ingredients, the aim of the present work was to evaluate the effects of freezing, oven-drying and freeze-drying on chemical composition of spirulina biomass. Total proteins, photosynthetic pigments and antioxidants, were analyzed and compared to respective quantities in fresh biomass. The frozen sample exhibited the highest content of phycocyanin-C, phenols, and ascorbic acid, also respect to the fresh biomass. The highest total flavonoid amount was in the freeze-dried biomass. HPLC-DAD analysis of phenolic acids revealed the presence of the isoflavone genistein, known for its therapeutic role, in all the spirulina samples. The phosphomolybdenum method (TAC) and DPPH scavenging activity were applied to determine the antioxidant activity of different samples. The highest DPPH scavenging activity was detected in fresh and freeze-dried biomass and it was positively related to carotenoid content. A positive correlation indicated that carotenoids, chlorophyll, ascorbic acid and all phenolic compounds were the major contributors to the TAC activity in spirulina biomass. The results highlighted a different functional value of spirulina biomass, depending on the processing methods used for its storage.

show abstract

Investigation of the effect of biologically active threo-Ds-isocitric acid on oxidative stress in Paramecium caudatum

Cited by 26 publications

References 32 publications

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations

Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass

Contact Info

Product

Resources

About