Investigation of the effect of pressure on retention of small molecules using reversed-phase ultra-high-pressure liquid chromatography

Influence of pressure on the retention of sugars in hydrophilic interaction chromatography

“…(1) involves a change in the stationary phase volume as a function of pressure. The change was small for RP packings [11], and is completely negligible for the case studied here. The retention mechanism for sugars is the partitioning into a water layer on the surface of the packing [18], and the compressibility of water is very small.…”

“…(1) to describe the retention changes for the sugars: Figure 1 shows the chromatograms for the sugars as a function of pressure, and Table 1 presents the analysis of the retention data. In agreement with the data treatment in references [11] and [12], the pressure data reported in the caption of Fig. 1 were corrected for half the column backpressure, which was only 4.7 bar under the test conditions.…”

“…For the RP retention mechanism of this study, increases in retention were observed. This was also true for the more detailed and expanded studies in references [11,12]. Fallas, McCalley and coworkers [11,12] examined the retention changes of a wide range of analytes with variable mobile phase conditions in RP chromatography using Ultra-Performance Liquid Chromatography s (UPLC s ) instrumentation, which permitted the exploration of a wider pressure range.…”

“…This was also true for the more detailed and expanded studies in references [11,12]. Fallas, McCalley and coworkers [11,12] examined the retention changes of a wide range of analytes with variable mobile phase conditions in RP chromatography using Ultra-Performance Liquid Chromatography s (UPLC s ) instrumentation, which permitted the exploration of a wider pressure range. Also, Gritti and Guiochon recently further explored the mass transfer and retention behavior of insulin as a function of temperature and pressure on a RP packing, now at UPLC s pressures [13].…”

Maximizing the peak capacity using coupled columns packed with 2.6μm core–shell particles operated at 1200bar

“…3.1% decrease in k last when increasing F from 0.1 to 1.2 mL/min). These deviations can be attributed to the effect of the higher degree of viscous heating [18,26,27] accompanying the higher flow rate. Such effects are typical for any change to a higher pressure system and cannot be captured in a simple rule such as the constant 0 -and ˇ·t 0 -rule (see also discussion of Figs.…”